AFR1_YEAST
ID AFR1_YEAST Reviewed; 620 AA.
AC P33304; D6VS72;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Protein AFR1;
GN Name=AFR1; OrderedLocusNames=YDR085C; ORFNames=D4471;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8413281; DOI=10.1128/mcb.13.11.6876-6888.1993;
RA Konopka J.B.;
RT "AFR1 acts in conjunction with the alpha-factor receptor to promote
RT morphogenesis and adaptation.";
RL Mol. Cell. Biol. 13:6876-6888(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472 AND SER-526, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Acts in conjunction with the alpha-factor receptor to promote
CC morphogenesis and adaptation.
CC -!- INDUCTION: By pheromone (alpha-factor).
CC -!- SIMILARITY: To yeast YER158C. {ECO:0000305}.
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DR EMBL; Z46796; CAA86807.1; -; Genomic_DNA.
DR EMBL; L21702; AAC37365.1; -; Unassigned_DNA.
DR EMBL; X82086; CAA57614.1; -; Genomic_DNA.
DR EMBL; Z74381; CAA98905.1; -; Genomic_DNA.
DR EMBL; AY723775; AAU09692.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11932.1; -; Genomic_DNA.
DR PIR; S48772; S48772.
DR RefSeq; NP_010370.1; NM_001180393.1.
DR AlphaFoldDB; P33304; -.
DR BioGRID; 32141; 46.
DR DIP; DIP-824N; -.
DR IntAct; P33304; 7.
DR MINT; P33304; -.
DR STRING; 4932.YDR085C; -.
DR iPTMnet; P33304; -.
DR PaxDb; P33304; -.
DR PRIDE; P33304; -.
DR EnsemblFungi; YDR085C_mRNA; YDR085C; YDR085C.
DR GeneID; 851658; -.
DR KEGG; sce:YDR085C; -.
DR SGD; S000002492; AFR1.
DR VEuPathDB; FungiDB:YDR085C; -.
DR eggNOG; ENOG502S3XF; Eukaryota.
DR HOGENOM; CLU_440893_0_0_1; -.
DR InParanoid; P33304; -.
DR OMA; ESARYFY; -.
DR BioCyc; YEAST:G3O-29690-MON; -.
DR PRO; PR:P33304; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P33304; protein.
DR GO; GO:0001400; C:mating projection base; IDA:SGD.
DR GO; GO:0003779; F:actin binding; IBA:GO_Central.
DR GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0000753; P:cell morphogenesis involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0097271; P:protein localization to bud neck; IMP:SGD.
DR GO; GO:0032185; P:septin cytoskeleton organization; IMP:SGD.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..620
FT /note="Protein AFR1"
FT /id="PRO_0000064485"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 472
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 526
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
SQ SEQUENCE 620 AA; 71364 MW; A9ABFBC9A26E62E5 CRC64;
MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN PNKKRHIYSG
EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY HEGFNNDQDY VSQYQKPQYT
FGVYKELTPY QLQRSKMKRS FQFPNGEIYK PKLDGKCTHS LKKPELNSRD SSLFKFSEKK
GRNLSKDFVG PHNGTSVIHI PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS
LPESQTDDDD GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK
TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID DMVTHSDGNS
EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND KYDLRRRQLE IRQKLHETSH
NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS KLGEYIIDTR NQPPPRSSKR PSLDDNESAR
YFYNISTDLR QSLSGPISLP MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK
PIKKDVRFAK EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS
YKKNEMVVHE NSKCFTHYLI
