EHD2_ORYSJ
ID EHD2_ORYSJ Reviewed; 475 AA.
AC B1B534; A3C4T1; Q7XEJ8;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein EARLY HEADING DATE 2 {ECO:0000303|PubMed:18790997};
DE Short=Ehd2 {ECO:0000303|PubMed:18790997};
DE AltName: Full=Protein RICE INDETERMINATE 1 {ECO:0000303|PubMed:18725639};
DE Short=OsID {ECO:0000303|PubMed:16784536};
DE Short=OsID1 {ECO:0000303|PubMed:18774969};
GN Name=EHD2 {ECO:0000303|PubMed:18790997};
GN Synonyms=GHD10 {ECO:0000303|PubMed:24280027},
GN ID {ECO:0000303|PubMed:16784536}, ID1 {ECO:0000303|PubMed:18774969},
GN RID1 {ECO:0000303|PubMed:18725639};
GN OrderedLocusNames=Os10g0419200 {ECO:0000312|EMBL:BAT10873.1},
GN LOC_Os10g28330 {ECO:0000305};
GN ORFNames=OSNPB_100419200 {ECO:0000312|EMBL:BAT10873.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Tohoku IL9;
RX PubMed=18790997; DOI=10.1104/pp.108.125542;
RA Matsubara K., Yamanouchi U., Wang Z.-X., Minobe Y., Izawa T., Yano M.;
RT "Ehd2, a rice ortholog of the maize INDETERMINATE1 gene, promotes flowering
RT by up-regulating Ehd1.";
RL Plant Physiol. 148:1425-1435(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE,
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Zhonghua 11;
RX PubMed=18725639; DOI=10.1073/pnas.0806019105;
RA Wu C., You C., Li C., Long T., Chen G., Byrne M.E., Zhang Q.;
RT "RID1, encoding a Cys2/His2-type zinc finger transcription factor, acts as
RT a master switch from vegetative to floral development in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:12915-12920(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16784536; DOI=10.1186/1471-2164-7-158;
RA Colasanti J., Tremblay R., Wong A.Y., Coneva V., Kozaki A., Mable B.K.;
RT "The maize INDETERMINATE1 flowering time regulator defines a highly
RT conserved zinc finger protein family in higher plants.";
RL BMC Genomics 7:158-158(2006).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=18774969; DOI=10.1111/j.1365-313x.2008.03667.x;
RA Park S.J., Kim S.L., Lee S., Je B.I., Piao H.L., Park S.H., Kim C.M.,
RA Ryu C.-H., Park S.H., Xuan Y.H., Colasanti J., An G., Han C.D.;
RT "Rice Indeterminate 1 (OsId1) is necessary for the expression of Ehd1
RT (Early heading date 1) regardless of photoperiod.";
RL Plant J. 56:1018-1029(2008).
RN [10]
RP REVIEW ON FLOWERING.
RX PubMed=19304997; DOI=10.1093/aob/mcp063;
RA Greenup A., Peacock W.J., Dennis E.S., Trevaskis B.;
RT "The molecular biology of seasonal flowering-responses in Arabidopsis and
RT the cereals.";
RL Ann. Bot. 103:1165-1172(2009).
RN [11]
RP FUNCTION, MUTAGENESIS OF PRO-158, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Wuyunjing 7;
RX PubMed=24280027; DOI=10.1186/1939-8433-6-24;
RA Hu S., Dong G., Xu J., Su Y., Shi Z., Ye W., Li Y., Li G., Zhang B., Hu J.,
RA Qian Q., Zeng D., Guo L.;
RT "A point mutation in the zinc finger motif of RID1/EHD2/OsID1 protein leads
RT to outstanding yield-related traits in japonica rice variety Wuyunjing 7.";
RL Rice 6:24-24(2013).
RN [12]
RP REPRESSION BY DLF1.
RX PubMed=25036785; DOI=10.1371/journal.pone.0102529;
RA Cai Y., Chen X., Xie K., Xing Q., Wu Y., Li J., Du C., Sun Z., Guo Z.;
RT "Dlf1, a WRKY transcription factor, is involved in the control of flowering
RT time and plant height in rice.";
RL PLoS ONE 9:E102529-E102529(2014).
RN [13]
RP INDUCTION BY FKF1.
RX PubMed=25850808; DOI=10.1111/pce.12549;
RA Han S.-H., Yoo S.-C., Lee B.-D., An G., Paek N.-C.;
RT "Rice FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (OsFKF1) promotes flowering
RT independent of photoperiod.";
RL Plant Cell Environ. 38:2527-2540(2015).
RN [14]
RP REGULATION BY PHYTOCHROME.
RX PubMed=25573482; DOI=10.1038/srep07709;
RA Yoshitake Y., Yokoo T., Saito H., Tsukiyama T., Quan X., Zikihara K.,
RA Katsura H., Tokutomi S., Aboshi T., Mori N., Inoue H., Nishida H.,
RA Kohchi T., Teraishi M., Okumoto Y., Tanisaka T.;
RT "The effects of phytochrome-mediated light signals on the developmental
RT acquisition of photoperiod sensitivity in rice.";
RL Sci. Rep. 5:7709-7709(2015).
RN [15]
RP REVIEW ON FLOWERING.
RX PubMed=28491078; DOI=10.3389/fpls.2017.00665;
RA Brambilla V., Gomez-Ariza J., Cerise M., Fornara F.;
RT "The importance of being on time: Regulatory networks controlling
RT photoperiodic flowering in cereals.";
RL Front. Plant Sci. 8:665-665(2017).
CC -!- FUNCTION: Transcription activator that acts as a flowering master
CC switch in both long and short days, independently of the circadian
CC clock (PubMed:18790997, PubMed:18725639, PubMed:18774969,
CC PubMed:19304997, PubMed:24280027). Promotes flowering upstream of HD1
CC by up-regulating FTL1, FTL4, FTL5, FTL6, EHD1, HD3A and RFT1
CC (PubMed:18790997, PubMed:18725639, PubMed:18774969). Seems to repress
CC FTL11 expression (PubMed:18725639). May recognize the consensus motif
CC 5'-TTTGTCGTAAT-3' in target gene promoters (PubMed:18725639).
CC {ECO:0000269|PubMed:18725639, ECO:0000269|PubMed:18774969,
CC ECO:0000269|PubMed:18790997, ECO:0000269|PubMed:24280027,
CC ECO:0000303|PubMed:19304997}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:18725639, ECO:0000269|PubMed:18774969,
CC ECO:0000269|PubMed:24280027}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in developing leaves (more in
CC sheaths than in blades, especially in the outer epidermal cell of
CC immature leaves and in the region immediately beneath the meristem
CC where internodes are visible) and panicles, and, at very low levels,
CC around the shoot apex and in roots. {ECO:0000269|PubMed:18725639,
CC ECO:0000269|PubMed:18774969, ECO:0000269|PubMed:18790997,
CC ECO:0000269|PubMed:24280027}.
CC -!- DEVELOPMENTAL STAGE: Detected in young expanded leaves emerging from
CC the culms (PubMed:18774969). In young panicles, accumulates in
CC spikelets and panicle branches (PubMed:18774969). Under short days
CC (SD), observed in leaves by 1 week after germination and reaches a peak
CC by 2 weeks. Subsequently, the level gradually decreases, but maintains
CC at low levels even after flowering (about 9 weeks) (PubMed:18790997).
CC Under long days (LD), present in leaves at low levels during all
CC developmental stages from at least 1 week after germination
CC (PubMed:18790997). {ECO:0000269|PubMed:18774969,
CC ECO:0000269|PubMed:18790997}.
CC -!- INDUCTION: Induced by FKF1 (PubMed:25850808). Down-regulated by DLF1
CC (PubMed:25036785). During the basic vegetative growth phase (BVP,
CC photoperiod-insensitive phase), suppressed via phytochrome-mediated
CC light signals involving the phytochromobilin synthase HY2
CC (PubMed:25573482). {ECO:0000269|PubMed:25036785,
CC ECO:0000269|PubMed:25573482, ECO:0000269|PubMed:25850808}.
CC -!- DISRUPTION PHENOTYPE: Never-flowering phenotype in the rid1 mutant
CC (PubMed:18725639). Extremely delayed flowering under both short- (SD)
CC and long-day (LD) conditions, associated with reduced EHD1, HD3A and
CC RFT1 levels, but increased leaves and nodes production
CC (PubMed:18790997, PubMed:18725639, PubMed:18774969). Levels of FTL1 and
CC FTL4 are slightly reduced under both LD and SD, expression of FTL5 is
CC down-regulated under LD, whereas FTL6 is down-regulated only under SD
CC (PubMed:18725639). In contrast, the accumulation of FTL11 is slightly
CC higher under both SD and LD (PubMed:18725639). Reduced levels of MADS1,
CC MADS14 and MADS15, downstream genes of EHD1 (PubMed:18774969).
CC {ECO:0000269|PubMed:18725639, ECO:0000269|PubMed:18774969,
CC ECO:0000269|PubMed:18790997}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP53791.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAF26530.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAZ16094.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ16094.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB359195; BAG12102.1; -; Genomic_DNA.
DR EMBL; AB359196; BAG12103.1; -; mRNA.
DR EMBL; FJ009578; ACH87394.1; -; mRNA.
DR EMBL; FJ009579; ACH87395.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP53791.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008216; BAF26530.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014966; BAT10873.1; -; Genomic_DNA.
DR EMBL; CM000147; EAZ16094.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015614407.1; XM_015758921.1.
DR AlphaFoldDB; B1B534; -.
DR STRING; 4530.OS10T0419200-00; -.
DR PaxDb; B1B534; -.
DR PRIDE; B1B534; -.
DR EnsemblPlants; Os10t0419200-01; Os10t0419200-01; Os10g0419200.
DR GeneID; 4348644; -.
DR Gramene; Os10t0419200-01; Os10t0419200-01; Os10g0419200.
DR KEGG; osa:4348644; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_014578_4_2_1; -.
DR OMA; ATTVACC; -.
DR OrthoDB; 1318335at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0048574; P:long-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0048575; P:short-day photoperiodism, flowering; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Activator; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..475
FT /note="Protein EARLY HEADING DATE 2"
FT /id="PRO_0000447325"
FT ZN_FING 105..127
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 155..185
FT /note="C2H2-type 2"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT ZN_FING 190..213
FT /note="C2H2-type 2; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 217..240
FT /note="CCHC-type 2; atypical"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..239
FT /note="SHR-binding"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT MOTIF 123..130
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 177..184
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 192
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 195
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 208
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 212
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 221
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 234
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT BINDING 238
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q700D2"
FT MUTAGEN 158
FT /note="P->L: In ghd10; delayed flowering time, tall stalks
FT and increased panicle length and primary branch number,
FT thus leading to increased grains yield. Reduced expression
FT of EHD1, HD1, RFT1, HD3A and MADS15 under both short-days
FT (SD) and long-days (LD) conditions."
FT /evidence="ECO:0000269|PubMed:24280027"
SQ SEQUENCE 475 AA; 50672 MW; 107CE5B7004BF4A1 CRC64;
MLLSDLSSDQ EATGSNSHGG GGGDRMVVGS HGAAHVVLSN LFLPPAAAAA ATMLLPAAPV
MVRPAAMAAA QEPRAKKKRS LPGNPDPEAE VIALSPRALV ATNRFVCEVC NKGFQRDQNL
QLHRRGHNLP WKLRHRAAAV SAVTTAAPAP RKRVYVCPEP TCVHHDPARA LGDLTGIKKH
FSRKHGEKRW RCERCGKRYA VHSDWKAHVK NCGTREYRCD CGILFSRKDS LLTHRAFCDA
LAEESARLLA AANNSSSITT TTCNNSNISS NNNNNNINSI SNSNNLLITS SSSSPPLFLP
FSTTPAENPN PNQLLFLQQH QAAHHQLLLP QFQQPPSSPP AYFDHLAFGG GGGVITGSSC
NDDNSSIAGD VMVAAGGDSV SFGLTSEGSV TMHAGDVGRR RLTRDFLGVD HDAGEVDELE
LDELPADLST TAAACQGCNF AAATTAACCA TDFTTGSRQY LGRLPPVNET WSHNF
