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EHD3_RAT
ID   EHD3_RAT                Reviewed;         535 AA.
AC   Q8R491;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=EH domain-containing protein 3 {ECO:0000305};
GN   Name=Ehd3 {ECO:0000312|RGD:621762}; Synonyms=Ehd2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Adipose tissue;
RA   Park S.Y., Lee W.;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   INTERACTION WITH PACSIN1.
RX   PubMed=15930129; DOI=10.1091/mbc.e05-01-0076;
RA   Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D.,
RA   Kessels M.M., Qualmann B.;
RT   "EHD proteins associate with syndapin I and II and such interactions play a
RT   crucial role in endosomal recycling.";
RL   Mol. Biol. Cell 16:3642-3658(2005).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349 AND SER-456, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: ATP- and membrane-binding protein that controls membrane
CC       reorganization/tubulation upon ATP hydrolysis. In vitro causes
CC       tubulation of endocytic membranes. Binding to phosphatidic acid induces
CC       its membrane tubulation activity. Plays a role in endocytic transport.
CC       Involved in early endosome to recycling endosome compartment (ERC),
CC       retrograde early endosome to Golgi, and endosome to plasma membrane
CC       (rapid recycling) protein transport. Involved in the regulation of
CC       Golgi maintenance and morphology. Involved in the recycling of
CC       internalized D1 dopamine receptor (By similarity). Plays a role in
CC       cardiac protein trafficking probably implicating ANK2. Involved in the
CC       ventricular membrane targeting of SLC8A1 and CACNA1C and probably the
CC       atrial membrane localization of CACNA1GG and CACNA1H implicated in the
CC       regulation of atrial myocyte excitability and cardiac conduction. In
CC       conjunction with EHD4 may be involved in endocytic trafficking of
CC       KDR/VEGFR2 implicated in control of glomerular function. Involved in
CC       the rapid recycling of integrin beta-3 implicated in cell adhesion
CC       maintenance. Involved in the unidirectional retrograde dendritic
CC       transport of endocytosed BACE1 and in efficient sorting of BACE1 to
CC       axons implicating a function in neuronal APP processing. Plays a role
CC       in the formation of the ciliary vesicle, an early step in cilium
CC       biogenesis; possibly sharing redundant functions with Ehd1.
CC       {ECO:0000250|UniProtKB:Q9NZN3, ECO:0000250|UniProtKB:Q9QXY6}.
CC   -!- SUBUNIT: Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4,
CC       ATP-binding is required for heterooligomerization (By similarity).
CC       Interacts with PACSIN1 (By similarity). Interacts with PACSIN2
CC       (PubMed:15930129). Interacts (via EH domain) with MICALL1. Interacts
CC       (via EH domain) with RAB11FIP2 (By similarity). Interacts with ANK2 (By
CC       similarity). Interacts with CACNA1GG and CACNA1H (By similarity).
CC       {ECO:0000250|UniProtKB:Q9NZN3, ECO:0000250|UniProtKB:Q9QXY6,
CC       ECO:0000269|PubMed:15930129}.
CC   -!- SUBCELLULAR LOCATION: Recycling endosome membrane
CC       {ECO:0000250|UniProtKB:Q9NZN3}; Peripheral membrane protein
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell membrane
CC       {ECO:0000250|UniProtKB:Q9NZN3}; Peripheral membrane protein
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell projection, cilium
CC       membrane {ECO:0000250|UniProtKB:Q9NZN3}; Peripheral membrane protein
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Localizes to the
CC       ciliary pocket from where the cilium protrudes. Colocalizes with RAB8A
CC       and MYO5B to a cytoplasmic tubular network devoid of RAB11A.
CC       Colocalizes with ANK2 in myocyte perinuclear region. Colocalizes with
CC       BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1
CC       and APP amyloid beta proteins in hippocampal mossy fiber terminals.
CC       {ECO:0000250|UniProtKB:Q9NZN3, ECO:0000250|UniProtKB:Q9QXY6}.
CC   -!- DOMAIN: The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target
CC       proteins. {ECO:0000250|UniProtKB:Q9WVK4}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC       superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU01055}.
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DR   EMBL; AF494093; AAM14604.2; -; mRNA.
DR   RefSeq; NP_620245.2; NM_138890.2.
DR   AlphaFoldDB; Q8R491; -.
DR   BMRB; Q8R491; -.
DR   SMR; Q8R491; -.
DR   BioGRID; 251376; 1.
DR   IntAct; Q8R491; 2.
DR   MINT; Q8R491; -.
DR   STRING; 10116.ENSRNOP00000010419; -.
DR   iPTMnet; Q8R491; -.
DR   PhosphoSitePlus; Q8R491; -.
DR   jPOST; Q8R491; -.
DR   PaxDb; Q8R491; -.
DR   PRIDE; Q8R491; -.
DR   Ensembl; ENSRNOT00000010419; ENSRNOP00000010419; ENSRNOG00000007744.
DR   GeneID; 192249; -.
DR   KEGG; rno:192249; -.
DR   UCSC; RGD:621762; rat.
DR   CTD; 30845; -.
DR   RGD; 621762; Ehd3.
DR   eggNOG; KOG1954; Eukaryota.
DR   GeneTree; ENSGT00940000159274; -.
DR   HOGENOM; CLU_017595_1_1_1; -.
DR   InParanoid; Q8R491; -.
DR   PhylomeDB; Q8R491; -.
DR   TreeFam; TF314429; -.
DR   Reactome; R-RNO-983231; Factors involved in megakaryocyte development and platelet production.
DR   PRO; PR:Q8R491; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000007744; Expressed in frontal cortex and 19 other tissues.
DR   ExpressionAtlas; Q8R491; baseline and differential.
DR   Genevisible; Q8R491; RN.
DR   GO; GO:0020018; C:ciliary pocket membrane; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR   GO; GO:0030139; C:endocytic vesicle; ISO:RGD.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:RGD.
DR   GO; GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0034498; P:early endosome to Golgi transport; ISO:RGD.
DR   GO; GO:0032456; P:endocytic recycling; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR   GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR   GO; GO:0090160; P:Golgi to lysosome transport; ISO:RGD.
DR   GO; GO:1901387; P:positive regulation of voltage-gated calcium channel activity; ISO:RGD.
DR   GO; GO:0051260; P:protein homooligomerization; ISS:UniProtKB.
DR   GO; GO:0072659; P:protein localization to plasma membrane; ISO:RGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0001881; P:receptor recycling; ISO:RGD.
DR   GO; GO:1903779; P:regulation of cardiac conduction; ISO:RGD.
DR   GO; GO:0086036; P:regulation of cardiac muscle cell membrane potential; ISO:RGD.
DR   GO; GO:0055117; P:regulation of cardiac muscle contraction; ISO:RGD.
DR   GO; GO:1903358; P:regulation of Golgi organization; ISO:RGD.
DR   CDD; cd00052; EH; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR040990; DUF5600.
DR   InterPro; IPR045063; Dynamin_N.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR000261; EH_dom.
DR   InterPro; IPR029951; EHD1/EHD3.
DR   InterPro; IPR031692; EHD_N.
DR   InterPro; IPR030381; G_DYNAMIN_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11216:SF67; PTHR11216:SF67; 1.
DR   Pfam; PF18150; DUF5600; 1.
DR   Pfam; PF00350; Dynamin_N; 1.
DR   Pfam; PF12763; EF-hand_4; 1.
DR   Pfam; PF16880; EHD_N; 1.
DR   SMART; SM00027; EH; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
DR   PROSITE; PS50031; EH; 1.
DR   PROSITE; PS51718; G_DYNAMIN_2; 1.
PE   1: Evidence at protein level;
KW   Acetylation; ATP-binding; Calcium; Cell membrane; Cell projection; Cilium;
KW   Cilium biogenesis/degradation; Coiled coil; Endosome; Isopeptide bond;
KW   Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW   Protein transport; Reference proteome; Transport; Ubl conjugation.
FT   CHAIN           1..535
FT                   /note="EH domain-containing protein 3"
FT                   /id="PRO_0000322645"
FT   DOMAIN          55..286
FT                   /note="Dynamin-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   DOMAIN          444..532
FT                   /note="EH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00077"
FT   DOMAIN          476..511
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          65..72
FT                   /note="G1 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          91..92
FT                   /note="G2 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          153..156
FT                   /note="G3 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          219..222
FT                   /note="G4 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          243
FT                   /note="G5 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   COILED          198..227
FT                   /evidence="ECO:0000255"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZN3"
FT   BINDING         220
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q8BH64"
FT   BINDING         258
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q8BH64"
FT   BINDING         489
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         491
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         493
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         495
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         500
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZN3"
FT   MOD_RES         349
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         456
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   CROSSLNK        315
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZN3"
FT   CROSSLNK        511
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NZN3"
SQ   SEQUENCE   535 AA;  60791 MW;  A719748348CB87D9 CRC64;
     MFSWLGNDDR RKKDPEVFQT VSEGLKKLYK TKLLPLEEYY RFHEFHSPAL EDADFDNKPM
     VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDVEGIIP GNALVVDPKK
     PFRKLNAFGN AFLNRFVCAQ LPNAVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE
     RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL
     GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
     ARLAKVHAYI ISSLKKEMPS VFGKDTKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ
     DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ETQRPVQMVK GGAFEGTLQG
     PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV
     LGKIWKLADI DKDGMLDDEE FALANHLIKV KLEGHELPSE LPAHLLPPSK RKVAE
 
 
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