EHN3_CAEEL
ID EHN3_CAEEL Reviewed; 262 AA.
AC Q65XX7; H2L026; Q9N4M6;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Zinc finger protein ehn-3 {ECO:0000305};
DE AltName: Full=Enhancer of hand mutation protein 3 {ECO:0000312|WormBase:ZK616.10a};
GN Name=ehn-3 {ECO:0000312|WormBase:ZK616.10a};
GN ORFNames=ZK616.10 {ECO:0000312|WormBase:ZK616.10a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF ARG-41.
RX PubMed=12756172; DOI=10.1242/dev.00483;
RA Mathies L.D., Henderson S.T., Kimble J.;
RT "The C. elegans Hand gene controls embryogenesis and early gonadogenesis.";
RL Development 130:2881-2892(2003).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15294864; DOI=10.1242/dev.01288;
RA Mathies L.D., Schvarzstein M., Morphy K.M., Blelloch R., Spence A.M.,
RA Kimble J.;
RT "TRA-1/GLI controls development of somatic gonadal precursors in C.
RT elegans.";
RL Development 131:4333-4343(2004).
RN [4] {ECO:0000305}
RP FUNCTION (ISOFORMS A AND B), SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=20026024; DOI=10.1016/j.ydbio.2009.12.013;
RA Large E.E., Mathies L.D.;
RT "hunchback and Ikaros-like zinc finger genes control reproductive system
RT development in Caenorhabditis elegans.";
RL Dev. Biol. 339:51-64(2010).
RN [5] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=24402584; DOI=10.1534/g3.113.009852;
RA Large E.E., Mathies L.D.;
RT "Caenorhabditis elegans SWI/SNF subunits control sequential developmental
RT stages in the somatic gonad.";
RL G3 (Bethesda) 4:471-483(2014).
CC -!- FUNCTION: Together with the zinc finger protein ztf-16, plays a role in
CC gonadogenesis, specifically in somatic gonad precursor cell development
CC (PubMed:12756172, PubMed:15294864, PubMed:20026024, PubMed:24402584).
CC This is possibly by regulating tra-1 gene expression (PubMed:15294864).
CC {ECO:0000269|PubMed:12756172, ECO:0000269|PubMed:15294864,
CC ECO:0000269|PubMed:20026024, ECO:0000269|PubMed:24402584}.
CC -!- FUNCTION: [Isoform a]: Required for proper gonadal primordium assembly
CC and somatic gonad precursor cell morphology.
CC {ECO:0000269|PubMed:20026024}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15294864,
CC ECO:0000269|PubMed:20026024}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:ZK616.10a};
CC IsoId=Q65XX7-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:ZK616.10b};
CC IsoId=Q65XX7-2; Sequence=VSP_060360;
CC Name=c {ECO:0000312|WormBase:ZK616.10c};
CC IsoId=Q65XX7-3; Sequence=VSP_060360, VSP_060361;
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis (at protein level)
CC (PubMed:20026024). First expressed shortly after the assembly of the
CC gonad primordium (PubMed:15294864). Expressed in somatic gonadal
CC precursor (SGP) cells in embryos and L1 stage larvae (PubMed:20026024,
CC PubMed:24402584). Expressed in daughter cells of SGP cells throughout
CC the L1 larval stage (PubMed:20026024). {ECO:0000269|PubMed:15294864,
CC ECO:0000269|PubMed:20026024, ECO:0000269|PubMed:24402584}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in abnormal
CC gonadal development in 10% of animals whereby gonads fail to divide or
CC grow in size (PubMed:15294864). This abnormality is enhanced in a tra-1
CC (e1099) mutant background and is accompanied by masculinization of
CC gonadal tissues (PubMed:15294864). {ECO:0000269|PubMed:15294864}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; BX284604; CCD71116.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD71117.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD71118.1; -; Genomic_DNA.
DR RefSeq; NP_001023615.1; NM_001028444.1. [Q65XX7-1]
DR RefSeq; NP_001023616.1; NM_001028445.1. [Q65XX7-2]
DR RefSeq; NP_001023617.1; NM_001028446.1. [Q65XX7-3]
DR AlphaFoldDB; Q65XX7; -.
DR IntAct; Q65XX7; 44.
DR STRING; 6239.ZK616.10a; -.
DR PaxDb; Q65XX7; -.
DR EnsemblMetazoa; ZK616.10a.1; ZK616.10a.1; WBGene00001223. [Q65XX7-1]
DR EnsemblMetazoa; ZK616.10b.1; ZK616.10b.1; WBGene00001223. [Q65XX7-2]
DR EnsemblMetazoa; ZK616.10c.1; ZK616.10c.1; WBGene00001223. [Q65XX7-3]
DR GeneID; 191363; -.
DR KEGG; cel:CELE_ZK616.10; -.
DR UCSC; ZK616.10a; c. elegans.
DR CTD; 191363; -.
DR WormBase; ZK616.10a; CE37599; WBGene00001223; ehn-3. [Q65XX7-1]
DR WormBase; ZK616.10b; CE26343; WBGene00001223; ehn-3. [Q65XX7-2]
DR WormBase; ZK616.10c; CE37600; WBGene00001223; ehn-3. [Q65XX7-3]
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00720000109290; -.
DR HOGENOM; CLU_016943_1_0_1; -.
DR InParanoid; Q65XX7; -.
DR OMA; SAHLICH; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q65XX7; -.
DR PRO; PR:Q65XX7; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00001223; Expressed in embryo and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008406; P:gonad development; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..262
FT /note="Zinc finger protein ehn-3"
FT /id="PRO_0000448222"
FT ZN_FING 2..24
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 30..52
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 59..84
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 92..115
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 208..230
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 236..260
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 179..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..193
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform b and isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060360"
FT VAR_SEQ 120..162
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060361"
FT MUTAGEN 41
FT /note="R->W: In q689; some adult mutants show gonadal
FT defects, which become more prevalent on an hnd-1 mutant
FT background. Absent or misplaced somatic gonadal precursor
FT cells (SGPs) in the gonadal primordium during
FT embryogenesis."
FT /evidence="ECO:0000269|PubMed:12756172"
SQ SEQUENCE 262 AA; 30422 MW; E2913984465F0C1D CRC64;
MEKCDICHQK FSNKTNLNRH KVMHSGKKKF ECQFCRRPFF RNDRMKEHMM THIKKGNTFE
CPITACNSKF NSFTSLQFHV DSEHIIRGSS PAKCKSCIKW FNSSHRLLLH FHTAHLDHTK
FFSFKPAPIL PKSTTSILNP IKNPDDFDRI FDFFKTEIAP LFPLHSTVSQ APLPQCSRSV
KSAKELSPTP STEIETPEEE ELDGPESWYC DYCKIRFDDK VMWYLHSGLH SDDIPFKCSL
CGSLCDGKYD FAAHLVYANH NF
