ID   EI24_CAEEL              Reviewed;         315 AA.
AC   Q20123;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Ectopic P granules protein 4;
GN   Name=epg-4 {ECO:0000312|WormBase:F37C12.2};
GN   Synonyms=eip-24 {ECO:0000312|WormBase:F37C12.2}; ORFNames=F37C12.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=20550938; DOI=10.1016/j.cell.2010.04.034;
RA   Tian Y., Li Z., Hu W., Ren H., Tian E., Zhao Y., Lu Q., Huang X., Yang P.,
RA   Li X., Wang X., Kovacs A.L., Yu L., Zhang H.;
RT   "C. elegans screen identifies autophagy genes specific to multicellular
RT   organisms.";
RL   Cell 141:1042-1055(2010).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24374177; DOI=10.1016/j.devcel.2013.11.022;
RA   Manil-Segalen M., Lefebvre C., Jenzer C., Trichet M., Boulogne C.,
RA   Satiat-Jeunemaitre B., Legouis R.;
RT   "The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by
RT   interacting with the HOPS subunit VPS39.";
RL   Dev. Cell 28:43-55(2014).
CC   -!- FUNCTION: Involved in autophagy (PubMed:20550938, PubMed:24374177).
CC       Thought to act in autophagasome and omegasome formation
CC       (PubMed:20550938). {ECO:0000269|PubMed:20550938,
CC       ECO:0000269|PubMed:24374177}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20550938}. Membrane
CC       {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Note=Forms a
CC       reticular pattern throughout the cytoplasm.
CC   -!- TISSUE SPECIFICITY: Expressed in pharyngeal and body wall muscles and
CC       intestine cells. {ECO:0000269|PubMed:20550938}.
CC   -!- DEVELOPMENTAL STAGE: Ubiquitously expressed during embrygogenesis.
CC       {ECO:0000269|PubMed:20550938}.
CC   -!- DISRUPTION PHENOTYPE: Early autophagic structures and lgg-1 puncta form
CC       an irregular shape and clump together (PubMed:20550938). RNAi-mediated
CC       knockdown results in increased lgg-2-positive autophagosomes following
CC       fertilization and at later embryonic stages (PubMed:24374177).
CC       {ECO:0000269|PubMed:20550938, ECO:0000269|PubMed:24374177}.
CC   -!- SIMILARITY: Belongs to the EI24 family. {ECO:0000305}.
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DR   EMBL; FO081316; CCD70735.1; -; Genomic_DNA.
DR   PIR; T28836; T28836.
DR   RefSeq; NP_498575.1; NM_066174.4.
DR   AlphaFoldDB; Q20123; -.
DR   STRING; 6239.F37C12.2.3; -.
DR   EPD; Q20123; -.
DR   PaxDb; Q20123; -.
DR   PeptideAtlas; Q20123; -.
DR   EnsemblMetazoa; F37C12.2.1; F37C12.2.1; WBGene00018150.
DR   EnsemblMetazoa; F37C12.2.2; F37C12.2.2; WBGene00018150.
DR   EnsemblMetazoa; F37C12.2.3; F37C12.2.3; WBGene00018150.
DR   GeneID; 176009; -.
DR   UCSC; F37C12.2; c. elegans.
DR   CTD; 176009; -.
DR   WormBase; F37C12.2; CE00741; WBGene00018150; epg-4.
DR   eggNOG; KOG3966; Eukaryota.
DR   GeneTree; ENSGT00390000018633; -.
DR   HOGENOM; CLU_883479_0_0_1; -.
DR   InParanoid; Q20123; -.
DR   OMA; RPAERIY; -.
DR   OrthoDB; 889537at2759; -.
DR   PhylomeDB; Q20123; -.
DR   PRO; PR:Q20123; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00018150; Expressed in adult organism and 4 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:WormBase.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016236; P:macroautophagy; IMP:WormBase.
DR   GO; GO:1902902; P:negative regulation of autophagosome assembly; IMP:UniProtKB.
DR   InterPro; IPR009890; EI24.
DR   PANTHER; PTHR21389; PTHR21389; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Cytoplasm; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..315
FT                   /note="Ectopic P granules protein 4"
FT                   /id="PRO_0000404705"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   315 AA;  36559 MW;  1F8FCBB7CCACBE4A CRC64;
     MVKFQIIARD FYHGFIDSFK GITFVRRIRE EEAKEVKVEP PKPVERTVLM MRREKQGIFK
     RPPEPPKKKD SFLKKLWQIY AMNIGFLVLW QVCILILGLF FSFFDRTDLG HNIGYILIIP
     IFFASRIIQA LWFSDISGAC MRALKLPPPP VVPFSSMLAG TLISALHQIF FLIQGMLSQY
     LPIPLITPVI VYLHMALLNS MYCFDYFFDG YNLSFLRRKD IFESHWPYFL GFGTPLALAC
     SISSNMFVNS VIFALLFPFF IITSYPANWN RKYEEEIPKI AFCRISYMFT ELVGKFVKSI
     TPTNNPTAAR NNAQN