EI2BA_SCHPO
ID EI2BA_SCHPO Reviewed; 341 AA.
AC Q9USP0;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Translation initiation factor eIF-2B subunit alpha;
DE AltName: Full=eIF-2B GDP-GTP exchange factor subunit alpha;
GN Name=tif221; ORFNames=SPCC11E10.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Catalyzes the exchange of eukaryotic initiation factor 2-
CC bound GDP for GTP. {ECO:0000250}.
CC -!- SUBUNIT: Complex of five different subunits; alpha, beta, gamma, delta
CC and epsilon. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9USP0; P56286: tif211; NbExp=2; IntAct=EBI-1793476, EBI-16198594;
CC -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family.
CC {ECO:0000305}.
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DR EMBL; CU329672; CAB57849.1; -; Genomic_DNA.
DR PIR; T40858; T40858.
DR RefSeq; NP_588203.1; NM_001023193.2.
DR PDB; 5B04; X-ray; 2.99 A; A/B=1-341.
DR PDB; 6JLY; X-ray; 3.50 A; A/B=1-341.
DR PDB; 6JLZ; X-ray; 3.35 A; A/B=1-341.
DR PDBsum; 5B04; -.
DR PDBsum; 6JLY; -.
DR PDBsum; 6JLZ; -.
DR AlphaFoldDB; Q9USP0; -.
DR SMR; Q9USP0; -.
DR BioGRID; 275726; 20.
DR DIP; DIP-61958N; -.
DR IntAct; Q9USP0; 8.
DR MINT; Q9USP0; -.
DR STRING; 4896.SPCC11E10.07c.1; -.
DR iPTMnet; Q9USP0; -.
DR MaxQB; Q9USP0; -.
DR PaxDb; Q9USP0; -.
DR PRIDE; Q9USP0; -.
DR EnsemblFungi; SPCC11E10.07c.1; SPCC11E10.07c.1:pep; SPCC11E10.07c.
DR GeneID; 2539154; -.
DR KEGG; spo:SPCC11E10.07c; -.
DR PomBase; SPCC11E10.07c; tif221.
DR VEuPathDB; FungiDB:SPCC11E10.07c; -.
DR eggNOG; KOG1466; Eukaryota.
DR HOGENOM; CLU_016218_0_2_1; -.
DR InParanoid; Q9USP0; -.
DR OMA; LYPLGQY; -.
DR PhylomeDB; Q9USP0; -.
DR Reactome; R-SPO-72731; Recycling of eIF2:GDP.
DR PRO; PR:Q9USP0; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:PomBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:PomBase.
DR GO; GO:0003743; F:translation initiation factor activity; IDA:PomBase.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IDA:PomBase.
DR GO; GO:0010508; P:positive regulation of autophagy; IMP:PomBase.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR Gene3D; 1.20.120.1070; -; 1.
DR Gene3D; 3.40.50.10470; -; 1.
DR InterPro; IPR042528; elF-2B_alpha_N.
DR InterPro; IPR000649; IF-2B-related.
DR InterPro; IPR042529; IF_2B-like_C.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR Pfam; PF01008; IF-2B; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Initiation factor; Protein biosynthesis; Reference proteome.
FT CHAIN 1..341
FT /note="Translation initiation factor eIF-2B subunit alpha"
FT /id="PRO_0000156059"
FT HELIX 19..29
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:6JLZ"
FT HELIX 35..46
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 55..58
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 63..72
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 77..94
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 95..97
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 98..100
FT /evidence="ECO:0007829|PDB:6JLZ"
FT HELIX 101..109
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 110..114
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 115..129
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 130..132
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 137..143
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 146..157
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 162..167
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 170..173
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 174..184
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 189..192
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 194..196
FT /evidence="ECO:0007829|PDB:6JLZ"
FT HELIX 198..203
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 205..210
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 212..214
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 220..223
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 226..235
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 240..243
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 246..248
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 255..258
FT /evidence="ECO:0007829|PDB:6JLZ"
FT HELIX 299..302
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 307..311
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 313..315
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 317..321
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 324..326
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 328..330
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 331..339
FT /evidence="ECO:0007829|PDB:5B04"
SQ SEQUENCE 341 AA; 37773 MW; 6FAACEBEBD50995A CRC64;
MVESDSSGIV RHSQGEFDIV QVYKKFLQDD PEITMPVAAI EALVQLLSRS QAKTISEFMD
ILQNGSNTLK EGVQNNISLS AGCDIFQRFV TRSLHDVGDF EQCKRHLVEN GKLFIQRARA
CRQRIAHLGY PLIRDGSVIL THGFSRGVAA VLLAAAKRHV RFKVFVTESR PSGSGCLMTR
TLKNACIPTC MVLDSAVSFT MNRVDLVLVG AEGVVENGGL INQIGTFQLA VFAKHAHKPF
YAVAESHKFV RMFPLSQYDI PFSRPILEFD DPSPETVHPE PEPIPTPSDA IHNELIMNEE
QIRNNPTLDV TPPEFVSGLI TDLGIIDSKS GVSEELIKLY L
