AFR_RHIME
ID AFR_RHIME Reviewed; 333 AA.
AC Q92KZ3;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=1,5-anhydro-D-fructose reductase;
DE Short=Anhydrofructose reductase;
DE EC=1.1.1.292;
DE AltName: Full=1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming);
GN Name=afr; OrderedLocusNames=R03305; ORFNames=SMc04400;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D-
CC fructose to 1,5-anhydro-D-mannitol. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1,5-anhydro-D-mannitol + NADP(+) = 1,5-anhydro-D-fructose +
CC H(+) + NADPH; Xref=Rhea:RHEA:24208, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16715, ChEBI:CHEBI:49182, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.1.1.292;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Gfo/Idh/MocA family. {ECO:0000305}.
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DR EMBL; AL591688; CAC47884.1; -; Genomic_DNA.
DR RefSeq; NP_387411.1; NC_003047.1.
DR RefSeq; WP_003535887.1; NC_003047.1.
DR PDB; 4KOA; X-ray; 1.93 A; A=1-333.
DR PDBsum; 4KOA; -.
DR AlphaFoldDB; Q92KZ3; -.
DR SMR; Q92KZ3; -.
DR STRING; 266834.SMc04400; -.
DR EnsemblBacteria; CAC47884; CAC47884; SMc04400.
DR GeneID; 61604764; -.
DR KEGG; sme:SMc04400; -.
DR PATRIC; fig|266834.11.peg.4865; -.
DR eggNOG; COG0673; Bacteria.
DR HOGENOM; CLU_023194_1_3_5; -.
DR OMA; AWTIFLE; -.
DR BRENDA; 1.1.1.263; 5347.
DR BRENDA; 1.1.1.292; 5347.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0033712; F:1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) activity; IEA:UniProtKB-EC.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR InterPro; IPR004104; Gfo/Idh/MocA-like_OxRdtase_C.
DR InterPro; IPR000683; Gfo/Idh/MocA-like_OxRdtase_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01408; GFO_IDH_MocA; 1.
DR Pfam; PF02894; GFO_IDH_MocA_C; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW 3D-structure; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..333
FT /note="1,5-anhydro-D-fructose reductase"
FT /id="PRO_0000382697"
FT BINDING 9..12
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 33..34
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 38
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 71..76
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 93..94
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 120
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 162..163
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 283
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 11..15
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 17..23
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 27..32
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 36..45
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 49..53
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 55..59
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 66..69
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 76..85
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 100..113
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 127..137
FT /evidence="ECO:0007829|PDB:4KOA"
FT TURN 138..141
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 142..152
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 160..164
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 173..176
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 178..189
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 193..208
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 211..220
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 225..233
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 240..247
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 249..254
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 256..260
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 263..268
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 271..274
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 282..294
FT /evidence="ECO:0007829|PDB:4KOA"
FT STRAND 301..303
FT /evidence="ECO:0007829|PDB:4KOA"
FT HELIX 304..323
FT /evidence="ECO:0007829|PDB:4KOA"
SQ SEQUENCE 333 AA; 34852 MW; AB02C516327726F5 CRC64;
MIRWGLIGAS TIAREWVIGA IRAAGGEVVS VMSSSAERGE AYAAENGIAK AVTSVDDLVG
DPDVDAVYIS TTNELHHGQA LAAIRAGKHV LCEKPLAMNL NDGCEMVLKA CEAGVVLGTN
HHLRNAATHR AMREAIAAGR IGRPIAARVF HAVYLPPHLQ GWRLDKPEAG GGVILDITVH
DADTLRFVLN DDPIEAVAIS HSAGMGKEGL EDGVMGVLRF RSGVIAQFHD AFTTKFAETG
LEVHGTAGSL IGRNVMTQRP VGTVVLRNEE GESELPLDHR NLYETAIAAF HSAIGGNGRP
SASGEDGVWS LATGLAVVKA AATGGAVEIE TGL
