EI2BE_DICDI
ID EI2BE_DICDI Reviewed; 707 AA.
AC Q54RF3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Translation initiation factor eIF-2B subunit epsilon;
DE AltName: Full=eIF-2B GDP-GTP exchange factor subunit epsilon;
GN Name=eif2b5; ORFNames=DDB_G0283163;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Catalyzes the exchange of eukaryotic initiation factor 2-
CC bound GDP for GTP.
CC -!- SUBUNIT: Complex of five different subunits; alpha, beta, gamma, delta
CC and epsilon.
CC -!- SIMILARITY: Belongs to the eIF-2B gamma/epsilon subunits family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000051; EAL65820.1; -; Genomic_DNA.
DR RefSeq; XP_639192.1; XM_634100.1.
DR AlphaFoldDB; Q54RF3; -.
DR SMR; Q54RF3; -.
DR STRING; 44689.DDB0234243; -.
DR PaxDb; Q54RF3; -.
DR PRIDE; Q54RF3; -.
DR EnsemblProtists; EAL65820; EAL65820; DDB_G0283163.
DR GeneID; 8623968; -.
DR KEGG; ddi:DDB_G0283163; -.
DR dictyBase; DDB_G0283163; eif2b5.
DR eggNOG; KOG1461; Eukaryota.
DR HOGENOM; CLU_012507_1_0_1; -.
DR InParanoid; Q54RF3; -.
DR OMA; HYLYDKD; -.
DR PhylomeDB; Q54RF3; -.
DR Reactome; R-DDI-72731; Recycling of eIF2:GDP.
DR PRO; PR:Q54RF3; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005851; C:eukaryotic translation initiation factor 2B complex; ISS:dictyBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:dictyBase.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR CDD; cd04197; eIF-2B_epsilon_N; 1.
DR CDD; cd11558; W2_eIF2B_epsilon; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR035543; eIF-2B_epsilon_N.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR005835; NTP_transferase_dom.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR003307; W2_domain.
DR InterPro; IPR044123; W2_eIF2B_epsilon.
DR Pfam; PF00132; Hexapep; 1.
DR Pfam; PF00483; NTP_transferase; 1.
DR Pfam; PF02020; W2; 1.
DR SMART; SM00515; eIF5C; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR PROSITE; PS51363; W2; 1.
PE 3: Inferred from homology;
KW Reference proteome; Transferase.
FT CHAIN 1..707
FT /note="Translation initiation factor eIF-2B subunit
FT epsilon"
FT /id="PRO_0000328350"
FT DOMAIN 516..693
FT /note="W2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00695"
FT REGION 489..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 686..707
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 499..526
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 687..707
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 707 AA; 80253 MW; 3CFF8227A5370760 CRC64;
MSKNDKKNKQ SGSSNMDQLK SEDILQAVVL GDSFDRKFAP ITLEKPRTLL PLVNIPLLDY
TLEFLAASGV QQIFVFCCAH ASQIKEYIQS SRWHDLPGVQ VICMTGSNCR TTGDALRGVY
DAQVIQSDFI LISGDVVSNM NLQKALQIHK DRRELDKNNI MTMVYKQASS THRTRSKQDD
TVIWCNRDTM QVVCYDNSPS KKKSSISVEL FQKHPSIQMR YDLIDCHIDI CSPEVLALFN
DNFDFADIRK DFIHDILTSD LLDYKLSAYV LQGEYAARVK DLRTYHSVSK DIIHRWTFPM
VPDNNFMCNS SYSLSRQMIY KEKNVKLLGD CLISDETVIG TQTEIGAGSI VSHSTIGRNC
IIGKNVKING SYIWDDVTIQ DNAIIDHSII CNGSIIKSSS IIGRGSIIGF NVYIGQSKTL
EPFSKITMAQ YNEDEDDEEL LEEYFKEINL NDDNNNNNNN NNENNKTNRW LMENELYNEL
VPRINDSIHD DIESDESGDE GDKSGGKIKN NKNNDDNPIE PDSVKFHREV GDTIRRGIIE
KLPLENIQLE INGLKFAYDR DGLDCLTSIL PVLLESSSSS SSTTDSVTPK ELQQFIAGRI
SAFSPLLVKF SSEDSMVDLI FKIQDFCDEN EKFKVVFQPI LHQLYENDVI SEEAIFEWAE
EIEGDEEDDG FYLKKCKGFI DWLKSAEEES DDSDDSDDDD DDSDESD
