ID   AFSA_STRGG              Reviewed;         314 AA.
AC   B1VN93;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate synthase {ECO:0000305};
DE            EC=2.3.1.277 {ECO:0000269|PubMed:17277085};
DE   AltName: Full=A-factor biosynthesis enzyme {ECO:0000305};
GN   Name=afsA; OrderedLocusNames=SGR_6889 {ECO:0000312|EMBL:BAG23718.1};
OS   Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=455632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=18375553; DOI=10.1128/jb.00204-08;
RA   Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H.,
RA   Yamashita A., Hattori M., Horinouchi S.;
RT   "Genome sequence of the streptomycin-producing microorganism Streptomyces
RT   griseus IFO 13350.";
RL   J. Bacteriol. 190:4050-4060(2008).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=17277085; DOI=10.1073/pnas.0607472104;
RA   Kato J.Y., Funa N., Watanabe H., Ohnishi Y., Horinouchi S.;
RT   "Biosynthesis of gamma-butyrolactone autoregulators that switch on
RT   secondary metabolism and morphological development in Streptomyces.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2378-2383(2007).
CC   -!- FUNCTION: Involved in the biosynthesis of A factor (2-isocapryloyl-3R-
CC       hydroxymethyl-gamma-butyrolactone), a gamma-butyrolactone autoregulator
CC       that triggers secondary metabolism and morphogenesis in Streptomyces
CC       (PubMed:17277085). Catalyzes beta-ketoacyl transfer from 8-methyl-3-
CC       oxononanoyl-acyl carrier protein (ACP) to the hydroxyl group of
CC       dihydroxyacetone phosphate (DHAP), thus producing an 8-methyl-3-
CC       oxononanoyl-DHAP ester (PubMed:17277085).
CC       {ECO:0000269|PubMed:17277085}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a medium-chain 3-oxoacyl-[ACP] + dihydroxyacetone phosphate =
CC         a 2-oxo-3-(phosphooxy)propyl medium-chain 3-oxoalkanoate + holo-
CC         [ACP]; Xref=Rhea:RHEA:56860, Rhea:RHEA-COMP:9685, Rhea:RHEA-
CC         COMP:14764, ChEBI:CHEBI:57642, ChEBI:CHEBI:64479, ChEBI:CHEBI:141052,
CC         ChEBI:CHEBI:141053; EC=2.3.1.277;
CC         Evidence={ECO:0000269|PubMed:17277085};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=204.6 uM for DHAP {ECO:0000269|PubMed:17277085};
CC         Note=kcat is 2.0 min(-1). {ECO:0000269|PubMed:17277085};
CC       pH dependence:
CC         Optimum pH is 6.5-7.5. {ECO:0000269|PubMed:17277085};
CC       Temperature dependence:
CC         Optimum temperature is 15 degrees Celsius.
CC         {ECO:0000269|PubMed:17277085};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:17277085}.
CC   -!- SIMILARITY: Belongs to the AfsA family. {ECO:0000305}.
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DR   EMBL; AP009493; BAG23718.1; -; Genomic_DNA.
DR   RefSeq; WP_003971211.1; NC_010572.1.
DR   AlphaFoldDB; B1VN93; -.
DR   SMR; B1VN93; -.
DR   STRING; 455632.SGR_6889; -.
DR   EnsemblBacteria; BAG23718; BAG23718; SGR_6889.
DR   GeneID; 6214846; -.
DR   KEGG; sgr:SGR_6889; -.
DR   PATRIC; fig|455632.4.peg.7071; -.
DR   eggNOG; ENOG50342P2; Bacteria.
DR   HOGENOM; CLU_061800_0_0_11; -.
DR   OMA; NDHVPGM; -.
DR   OrthoDB; 1418394at2; -.
DR   Proteomes; UP000001685; Chromosome.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR005509; AfsA_hotdog_dom.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   Pfam; PF03756; AfsA; 2.
DR   SUPFAM; SSF54637; SSF54637; 1.
PE   1: Evidence at protein level;
KW   Transferase.
FT   CHAIN           1..314
FT                   /note="2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate synthase"
FT                   /id="PRO_0000450066"
SQ   SEQUENCE   314 AA;  34041 MW;  B034B9075A91A839 CRC64;
     MPEAAVLIDP VPTMDAEAEV VHPVGIEMVH RTRPEDAFPR NWVRLGRDRF AVEAVLPHDH
     PFFAPVGDDL HDPLLVAEAM RQAAMLAFHA GYGIPLGYHF LLTELDYVCH PEHLGVGGEP
     TEIGLEVFCS DLKWRAGLPA QGRVGWAVHR GDRLAATGVA ATRFSTPKAY RRMRGDVPVE
     GISLPETAPV PASPAGRARV EDVVLSGTGR EGVWELRVDT RHPTLFQRPN DHVPGMLLLE
     AARQAACLVA GPAGIVPVEA RTRFHRYSEF GSPCWIGAVV QPGADEDTVT VRVTGHQDGE
     TVFSTVLSGP RAHG