EI2BE_SCHPO
ID EI2BE_SCHPO Reviewed; 678 AA.
AC P56287;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable translation initiation factor eIF-2B subunit epsilon;
DE AltName: Full=eIF-2B GDP-GTP exchange factor subunit epsilon;
GN Name=tif225; ORFNames=SPAC8C9.15c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-172; SER-500; THR-503 AND
RP SER-506, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Subunit of the guanine nucleotide exchange factor for eIF-2.
CC {ECO:0000250}.
CC -!- SUBUNIT: Complex of five different subunits; alpha, beta, gamma, delta
CC and epsilon. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eIF-2B gamma/epsilon subunits family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB16302.1; -; Genomic_DNA.
DR PIR; T39151; T39151.
DR RefSeq; NP_594285.1; NM_001019708.2.
DR PDB; 5B04; X-ray; 2.99 A; I/J=1-678.
DR PDB; 6JLY; X-ray; 3.50 A; I/J=1-678.
DR PDB; 6JLZ; X-ray; 3.35 A; I/J=1-678.
DR PDBsum; 5B04; -.
DR PDBsum; 6JLY; -.
DR PDBsum; 6JLZ; -.
DR AlphaFoldDB; P56287; -.
DR SMR; P56287; -.
DR BioGRID; 278500; 4.
DR DIP; DIP-61962N; -.
DR IntAct; P56287; 3.
DR STRING; 4896.SPAC8C9.15c.1; -.
DR iPTMnet; P56287; -.
DR MaxQB; P56287; -.
DR PaxDb; P56287; -.
DR PRIDE; P56287; -.
DR EnsemblFungi; SPAC8C9.15c.1; SPAC8C9.15c.1:pep; SPAC8C9.15c.
DR GeneID; 2542017; -.
DR KEGG; spo:SPAC8C9.15c; -.
DR PomBase; SPAC8C9.15c; tif225.
DR VEuPathDB; FungiDB:SPAC8C9.15c; -.
DR eggNOG; KOG1461; Eukaryota.
DR HOGENOM; CLU_012507_1_0_1; -.
DR InParanoid; P56287; -.
DR OMA; HYLYDKD; -.
DR PhylomeDB; P56287; -.
DR Reactome; R-SPO-72731; Recycling of eIF2:GDP.
DR PRO; PR:P56287; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:PomBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:PomBase.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IDA:PomBase.
DR GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IDA:PomBase.
DR CDD; cd04197; eIF-2B_epsilon_N; 1.
DR CDD; cd11558; W2_eIF2B_epsilon; 1.
DR DisProt; DP02516; -.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR035543; eIF-2B_epsilon_N.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR005835; NTP_transferase_dom.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR011004; Trimer_LpxA-like_sf.
DR InterPro; IPR003307; W2_domain.
DR InterPro; IPR044123; W2_eIF2B_epsilon.
DR Pfam; PF00132; Hexapep; 2.
DR Pfam; PF00483; NTP_transferase; 1.
DR Pfam; PF02020; W2; 1.
DR SMART; SM00515; eIF5C; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF51161; SSF51161; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR PROSITE; PS51363; W2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Initiation factor; Phosphoprotein; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..678
FT /note="Probable translation initiation factor eIF-2B
FT subunit epsilon"
FT /id="PRO_0000156077"
FT DOMAIN 508..674
FT /note="W2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00695"
FT REGION 467..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 172
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 500
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 503
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 506
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:6JLZ"
FT STRAND 19..23
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:6JLZ"
FT TURN 32..35
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 40..42
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 43..49
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 50..61
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 74..83
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 108..115
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 137..147
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 153..161
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 171..173
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 174..179
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 181..183
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 185..191
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 204..209
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 212..226
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 229..236
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 237..239
FT /evidence="ECO:0007829|PDB:6JLZ"
FT TURN 243..246
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 247..252
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 260..266
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 277..288
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 290..292
FT /evidence="ECO:0007829|PDB:5B04"
FT HELIX 297..299
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 307..309
FT /evidence="ECO:0007829|PDB:5B04"
FT TURN 310..312
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 329..333
FT /evidence="ECO:0007829|PDB:6JLY"
FT STRAND 344..347
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 361..364
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 378..381
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 422..428
FT /evidence="ECO:0007829|PDB:5B04"
FT STRAND 432..434
FT /evidence="ECO:0007829|PDB:6JLZ"
FT STRAND 436..438
FT /evidence="ECO:0007829|PDB:5B04"
SQ SEQUENCE 678 AA; 76326 MW; D399547CBD82C4BC CRC64;
MPPSKGLNGK LEKPKHALQA IVLSDSYNYR FRPLTLDKPR CLLPLANTPL IEYTFEFLAL
AGVQEVYVFC CAHAGQIREY IEKSKWNLPS SPFSVNTIVS RESLSVGDAL RELDSKQLIT
SDFILVSGDV VSNVPLNEVL KEHRKRREDD KNAIMTMVVR EASPFHRTRA RTESSVFVID
KKTSQCVHYQ ANERGKHYVS MDPEIFNEHE ELEVRNDLID CQIDICSNDV PALFTENFDY
QDIRKDFVYG VLTSDLLGKK IHCHVAKENY AARVRSLQTY DAISKDVLSR WVYPFVPDSN
LLNQTFSYQR HQIYKEEDVV LARSCIIKAR TLIGAYTKVG DASVVANTII GRNCTIGSNC
SIDSAFLWED VVIGDNCRIG KAILANSVKI GNNCSIEDGA IVAAGVVIGD NTIIEKNKRL
TTFESHSQGT LNDPSLVGIG GRGQEYHAEE DSDDEGEFME ASGLIESTNE LHLSDSESSE
TSSSSEEDME FIPFSARRDS ANTINSEDFD EGDFNKEAQQ SLERAFEENH QIDIAALELN
TLRMAMNANY HEVRSAIVLA LLRRIMHLDV SPKEALAKVM TRWGPLLAKL TFSHEEQVDN
VLTLQKYCVR LSMTRHFLQL LGYFYQLEIA EENAIQEWYS DPRSSEGELA ALRDAGGKQF
VDWLNTAESE SESEEGSE
