ID   EI2BL_PYRHO             Reviewed;         276 AA.
AC   O58185;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Putative translation initiation factor eIF-2B subunit 2-like;
DE   AltName: Full=eIF-2B GDP-GTP exchange factor;
GN   OrderedLocusNames=PH0440;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Catalyzes the exchange of initiation factor 2-bound GDP for
CC       GTP. {ECO:0000250}.
CC   -!- SUBUNIT: Complex of two different subunits. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family.
CC       {ECO:0000305}.
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DR   EMBL; BA000001; BAA29526.1; -; Genomic_DNA.
DR   PIR; A71155; A71155.
DR   RefSeq; WP_010884550.1; NC_000961.1.
DR   PDB; 1VB5; X-ray; 2.20 A; A/B=1-276.
DR   PDBsum; 1VB5; -.
DR   AlphaFoldDB; O58185; -.
DR   SMR; O58185; -.
DR   STRING; 70601.3256843; -.
DR   EnsemblBacteria; BAA29526; BAA29526; BAA29526.
DR   GeneID; 1444337; -.
DR   KEGG; pho:PH0440; -.
DR   eggNOG; arCOG01125; Archaea.
DR   OMA; WKYIRGI; -.
DR   OrthoDB; 36100at2157; -.
DR   EvolutionaryTrace; O58185; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.420; -; 1.
DR   Gene3D; 3.40.50.10470; -; 1.
DR   InterPro; IPR000649; IF-2B-related.
DR   InterPro; IPR042529; IF_2B-like_C.
DR   InterPro; IPR027363; M1Pi_N.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   Pfam; PF01008; IF-2B; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Initiation factor; Protein biosynthesis.
FT   CHAIN           1..276
FT                   /note="Putative translation initiation factor eIF-2B
FT                   subunit 2-like"
FT                   /id="PRO_0000156109"
FT   HELIX           4..15
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           21..38
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   TURN            41..43
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           44..58
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           63..71
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           78..106
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          111..114
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           120..131
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          136..141
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   TURN            144..147
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           148..158
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          163..166
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           168..170
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           171..175
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          179..183
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          186..188
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          194..197
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           200..209
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          214..217
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           220..222
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           229..231
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          251..254
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   HELIX           256..258
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          260..264
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   STRAND          267..269
FT                   /evidence="ECO:0007829|PDB:1VB5"
FT   TURN            271..274
FT                   /evidence="ECO:0007829|PDB:1VB5"
SQ   SEQUENCE   276 AA;  31402 MW;  EBFA59BFDAE347A7 CRC64;
     MLPERVLEIL REMKRERIKG ASWLAKKGAE AFLTLAEELD ESLLEDAIME LREEVVKVNP
     SMASLYNLAR FIPVTNRRDI LKSRALEFLR RMEEAKRELA SIGAQLIDDG DVIITHSFSS
     TVLEIIRTAK ERKKRFKVIL TESSPDYEGL HLARELEFSG IEFEVITDAQ MGLFCREASI
     AIVGADMITK DGYVVNKAGT YLLALACHEN AIPFYVAAET YKFHPTLKSG DVMLMERDLI
     RGNVRIRNVL FDVTPWKYVR GIITELGIVI PPRDIQ