EI3EA_DANRE
ID EI3EA_DANRE Reviewed; 446 AA.
AC Q6DRI1; Q6P7X8; Q6TH16;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Eukaryotic translation initiation factor 3 subunit E-A {ECO:0000255|HAMAP-Rule:MF_03004};
DE Short=eIF3e-A {ECO:0000255|HAMAP-Rule:MF_03004};
DE AltName: Full=Eukaryotic translation initiation factor 3 subunit 6-A {ECO:0000255|HAMAP-Rule:MF_03004};
GN Name=eif3ea; Synonyms=eif3s6a, int6;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RX PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA Hopkins N.;
RT "Identification of 315 genes essential for early zebrafish development.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17895999; DOI=10.1371/journal.pone.0000959;
RA Grzmil M., Whiting D., Maule J., Anastasaki C., Amatruda J.F., Kelsh R.N.,
RA Norbury C.J., Patton E.E.;
RT "The INT6 cancer gene and MEK signaling pathways converge during zebrafish
RT development.";
RL PLoS ONE 2:E959-E959(2007).
CC -!- FUNCTION: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex, which is involved in protein synthesis of a
CC specialized repertoire of mRNAs and, together with other initiation
CC factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S
CC ribosome. The eIF-3 complex specifically targets and initiates
CC translation of a subset of mRNAs involved in cell proliferation.
CC {ECO:0000255|HAMAP-Rule:MF_03004}.
CC -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex, which is composed of 13 subunits: eif3a, eif3b, eif3c,
CC eif3d, eif3e, eif3f, eif3g, eif3h, eif3i, eif3j, eif3k, eif3l and
CC eif3m. {ECO:0000255|HAMAP-Rule:MF_03004}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03004}.
CC Nucleus {ECO:0000255|HAMAP-Rule:MF_03004}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing craniofacial tissues,
CC intestine and lens. {ECO:0000269|PubMed:17895999}.
CC -!- DISRUPTION PHENOTYPE: Reduced melanisation 2 in embryos 2 days post-
CC fertilization (dpf), misplaced pigment cells in the tail at 3 dpf, and
CC abnormal jaw morphogenesis at 4 dpf and 5 dpf.
CC {ECO:0000269|PubMed:17895999}.
CC -!- SIMILARITY: Belongs to the eIF-3 subunit E family. {ECO:0000255|HAMAP-
CC Rule:MF_03004}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ97773.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY648778; AAT68096.1; -; mRNA.
DR EMBL; AY398340; AAQ97773.1; ALT_FRAME; mRNA.
DR EMBL; BC061454; AAH61454.1; -; mRNA.
DR RefSeq; NP_957133.1; NM_200839.1.
DR AlphaFoldDB; Q6DRI1; -.
DR SMR; Q6DRI1; -.
DR STRING; 7955.ENSDARP00000106853; -.
DR PaxDb; Q6DRI1; -.
DR Ensembl; ENSDART00000064209; ENSDARP00000064208; ENSDARG00000113466.
DR Ensembl; ENSDART00000126705; ENSDARP00000106853; ENSDARG00000090697.
DR GeneID; 325102; -.
DR KEGG; dre:325102; -.
DR CTD; 325102; -.
DR ZFIN; ZDB-GENE-030131-3827; eif3ea.
DR eggNOG; KOG2758; Eukaryota.
DR GeneTree; ENSGT00390000002661; -.
DR HOGENOM; CLU_031132_0_1_1; -.
DR InParanoid; Q6DRI1; -.
DR OMA; VIMEPNR; -.
DR OrthoDB; 1151808at2759; -.
DR PhylomeDB; Q6DRI1; -.
DR TreeFam; TF101518; -.
DR Reactome; R-DRE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-DRE-72649; Translation initiation complex formation.
DR Reactome; R-DRE-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-DRE-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-DRE-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-DRE-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR PRO; PR:Q6DRI1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000090697; Expressed in pharyngeal gill and 23 other tissues.
DR ExpressionAtlas; Q6DRI1; baseline.
DR GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
DR GO; GO:0071540; C:eukaryotic translation initiation factor 3 complex, eIF3e; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0048856; P:anatomical structure development; IEA:UniProt.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0006413; P:translational initiation; ISS:UniProtKB.
DR HAMAP; MF_03004; eIF3e; 1.
DR InterPro; IPR016650; eIF3e.
DR InterPro; IPR019010; eIF3e_N.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10317; PTHR10317; 1.
DR Pfam; PF09440; eIF3_N; 1.
DR Pfam; PF01399; PCI; 1.
DR PIRSF; PIRSF016255; eIF3e_su6; 1.
DR SMART; SM01186; eIF3_N; 1.
DR SMART; SM00088; PINT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50250; PCI; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Initiation factor; Nucleus; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..446
FT /note="Eukaryotic translation initiation factor 3 subunit
FT E-A"
FT /id="PRO_0000365954"
FT DOMAIN 222..399
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT REGION 426..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 284
FT /note="Q -> H (in Ref. 1; AAT68096)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 446 AA; 52389 MW; 32F9D000E69F4F87 CRC64;
MAEYDLTTKI AHFLDRHLVF PLLEFLSVKE IYNEHELLHG KLDLLSDTNM VDFAMDVYRN
LFPDKEIPNS LREKRTTVVA QLKQLQSETE PIVKVFEDPE TTRQMQSTRD GRMLFDYLAD
KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP ATDRNALNSL WGKLASEILM
QNWEAAMEDL TRLRETIDNN TVSSPLQSLQ QRTWLIHWSL FVFFNHPKGR DNIIELFLYQ
PQYLNAIQTM CPHILRYLTT AVITNKDVRK RRQVLKDLVK VIQQESYTYK DPITEFVECL
YVNFDFDSAQ KKLRECEAVL VNDFFLVACL EDFIENARLF IFETFCRIHQ CISIGMLADK
LNMTPEEAER WIVNLIRNAR LDAKIDSKLG HVVMGNNAIS PYQQVIEKTK SLSFRSQMLA
MNIEKKQSNA NRNETPNWAA QDAGFY
