ID   AFSR_STRCO              Reviewed;         993 AA.
AC   P25941; P08467; Q9L000;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   18-OCT-2001, sequence version 2.
DT   03-AUG-2022, entry version 155.
DE   RecName: Full=Regulatory protein AfsR;
GN   Name=afsR; Synonyms=afsB; OrderedLocusNames=SCO4426; ORFNames=SCD6.04c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A3(2) / NRRL B-16638;
RX   PubMed=2253887; DOI=10.1016/0378-1119(90)90412-k;
RA   Horinouchi S., Kito M., Nishiyama M., Furuya K., Hong S.-K., Miyake K.,
RA   Beppu T.;
RT   "Primary structure of AfsR, a global regulatory protein for secondary
RT   metabolite formation in Streptomyces coelicolor A3(2).";
RL   Gene 95:49-56(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 751-993.
RC   STRAIN=A3(2) / NRRL B-16638;
RX   PubMed=2428809; DOI=10.1128/jb.168.1.257-269.1986;
RA   Horinouchi S., Suzuki H., Beppu T.;
RT   "Nucleotide sequence of afsB, a pleiotropic gene involved in secondary
RT   metabolism in Streptomyces coelicolor A3(2) and 'Streptomyces lividans'.";
RL   J. Bacteriol. 168:257-269(1986).
CC   -!- FUNCTION: Global regulatory protein for secondary metabolite formation.
CC   -!- DOMAIN: The N-terminal and the C-terminal regions are independently
CC       capable of directing actinorhodin production.
CC   -!- PTM: Phosphorylated by AfsK.
CC   -!- SIMILARITY: Belongs to the AfsR/DnrI/RedD regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; D90155; BAA14186.1; -; Genomic_DNA.
DR   EMBL; AL939120; CAB88433.1; -; Genomic_DNA.
DR   EMBL; M16011; AAA26694.1; -; Genomic_DNA.
DR   PIR; A25037; A25037.
DR   PIR; JQ0488; JQ0488.
DR   RefSeq; NP_628593.1; NC_003888.3.
DR   RefSeq; WP_011029645.1; NZ_VNID01000017.1.
DR   AlphaFoldDB; P25941; -.
DR   SMR; P25941; -.
DR   STRING; 100226.SCO4426; -.
DR   GeneID; 1099866; -.
DR   KEGG; sco:SCO4426; -.
DR   PATRIC; fig|100226.15.peg.4495; -.
DR   eggNOG; COG3629; Bacteria.
DR   eggNOG; COG3903; Bacteria.
DR   HOGENOM; CLU_004665_2_0_11; -.
DR   InParanoid; P25941; -.
DR   OMA; GCIGGDR; -.
DR   PhylomeDB; P25941; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0031409; F:pigment binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd15831; BTAD; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR005158; BTAD.
DR   InterPro; IPR002182; NB-ARC.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03704; BTAD; 1.
DR   Pfam; PF00931; NB-ARC; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM01043; BTAD; 1.
DR   SMART; SM00028; TPR; 4.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF48452; SSF48452; 2.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50005; TPR; 3.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   3: Inferred from homology;
KW   Activator; Antibiotic biosynthesis; ATP-binding; DNA-binding;
KW   Nucleotide-binding; Phosphoprotein; Pigment; Reference proteome; Repeat;
KW   TPR repeat; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..993
FT                   /note="Regulatory protein AfsR"
FT                   /id="PRO_0000110004"
FT   DOMAIN          280..579
FT                   /note="NB-ARC"
FT   REPEAT          823..856
FT                   /note="TPR 1"
FT   REPEAT          863..896
FT                   /note="TPR 2"
FT   REPEAT          942..975
FT                   /note="TPR 3"
FT   DNA_BIND        17..113
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   DNA_BIND        796..811
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   DNA_BIND        974..988
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         330..337
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        256
FT                   /note="Q -> R (in Ref. 1; BAA14186)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        318
FT                   /note="A -> P (in Ref. 1; BAA14186)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        751..766
FT                   /note="DVLLVSGRIEHAEEEA -> MCSWCPVASSMPRKRH (in Ref. 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   993 AA;  105716 MW;  8793CF27CEA7254C CRC64;
     MDGGPRVPEQ RRPGFPAEEE ESGALRFGVL GPVRAWRDGE TLATGSPQQR ALLAALLLRE
     GRTATAGELI DALWGEEPPS QALAAVRTYA SRLRKVLDPG VLVSESGGYA VRGLAEGALD
     LARAQDLASA AEKARSAGDL CHARDLLRRA LDLWDGEVLA GVPGPYAQTQ RVRLGEWRLQ
     LLETRLDMDL DQGCHAEAVS ELTALTAAHP LRERLRELLM LALYRSGRQA EALAVYADTR
     RLLADELGVD PRPGLQELQQ RILQADPALA ELSAATAETA TATLRPAQLP ATVSDFTGRA
     AFVRELSDVL SAASGESASG RVMAVSALAG IGGVGKTTLA VHVAHRARAA FPDGQLYVDL
     QGAGARPAEP ETVLGSFLRA LGTADSAIPD SLEERAALYR SVLDGRRVLV LLDNARDAAQ
     VRPLLPGTDG CAALVTARVR MVDLAGAHLV DLDVMAPEEA LALFTKIVGE ERVASERQAA
     LDVVGACGFL PLAIRIAASR LAARRTWTVS VLAAKLADER RRLDELQAGD QAVEATFELG
     YGQLEPAQAR AFRLLGLADG PDISLAAAAA VLDLPAQDTE DLLESLVDTS LLESAAPGRY
     RFHDLVRLYA RACAERTERD GNAPSERGAA LSRLLDFYLA TAAGVYAIER PGDRLVDGLE
     PTEYPGLTFT EGSAALDWLY TEAAPLLACV RQSAGTARLR RAVDLLWAAK DLTESGANSH
     QYEATARAMC DATGSAADTR AEGRARTVLS DVLLVSGRIE HAEEEARLAM RLAGSAEDSA
     AVSWVANNRG LVCLHQRRYA EGKGLFHQAI AGFRATDNRA GEASALSNLS RAQLGMGNVA
     EAVDIARQGL AVYAELGRTM RLANGHFALG VALTRAGRHE EALGEFAEAL DLFGDHRQRL
     WEGATNFRLA EVHLAAGRPS SAAQHAEQAL ALGCIGGDRM RGNVLALLGR ALSALGQADR
     ARACWREALS LYEQHDAQEV GEVRALLARS VAR