AFT1_YEAST
ID AFT1_YEAST Reviewed; 690 AA.
AC P22149; D6VU71; Q06993;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Iron-regulated transcriptional activator AFT1;
DE AltName: Full=Activator of iron transcription protein 1;
GN Name=AFT1; Synonyms=RCS1; OrderedLocusNames=YGL071W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=7720713; DOI=10.1002/j.1460-2075.1995.tb07106.x;
RA Yamaguchi-Iwai Y., Dancis A., Klausner R.D.;
RT "AFT1: a mediator of iron regulated transcriptional control in
RT Saccharomyces cerevisiae.";
RL EMBO J. 14:1231-1239(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9200812;
RX DOI=10.1002/(sici)1097-0061(19970615)13:7<621::aid-yea121>3.0.co;2-u;
RA Casas C., Aldea M., Espinet C., Gallego C., Gil R., Herrero E.;
RT "The AFT1 transcriptional factor is differentially required for expression
RT of high-affinity iron uptake genes in Saccharomyces cerevisiae.";
RL Yeast 13:621-637(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP NUCLEOTIDE SEQUENCE OF 283-690.
RC STRAIN=ATCC 26786 / X2180-1A;
RX PubMed=2021081; DOI=10.1002/yea.320070102;
RA Gil R., Zueco J., Sentandreu R., Herrero E.;
RT "RCS1, a gene involved in controlling cell size in Saccharomyces
RT cerevisiae.";
RL Yeast 7:1-14(1991).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26450372; DOI=10.1002/mbo3.303;
RA An X., Zhang C., Sclafani R.A., Seligman P., Huang M.;
RT "The late-annotated small ORF LSO1 is a target gene of the iron regulon of
RT Saccharomyces cerevisiae.";
RL MicrobiologyOpen 4:941-951(2015).
CC -!- FUNCTION: Probable transcription factor that activates the genes for
CC FRE1, FRE2 and FET3 in response to iron deprivation (PubMed:7720713,
CC PubMed:9200812). Also required for the expression of LSO1
CC (PubMed:26450372). Iron could interact directly with AFT1 and inhibits
CC its activity (PubMed:7720713). {ECO:0000269|PubMed:26450372,
CC ECO:0000269|PubMed:7720713, ECO:0000269|PubMed:9200812}.
CC -!- INTERACTION:
CC P22149; Q03835: GRX3; NbExp=4; IntAct=EBI-2332, EBI-22178;
CC P22149; P32642: GRX4; NbExp=2; IntAct=EBI-2332, EBI-22211;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Two-fold reduction in expression of LSO1.
CC {ECO:0000269|PubMed:26450372}.
CC -!- MISCELLANEOUS: Present with 2730 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA37215.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA54586.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Z48004; CAA88044.1; -; mRNA.
DR EMBL; X77413; CAA54586.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Z72593; CAA96775.1; -; Genomic_DNA.
DR EMBL; AY723805; AAU09722.1; -; Genomic_DNA.
DR EMBL; X53046; CAA37215.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006941; DAA08032.1; -; Genomic_DNA.
DR PIR; S54775; S54775.
DR RefSeq; NP_011444.1; NM_001180936.1.
DR AlphaFoldDB; P22149; -.
DR SMR; P22149; -.
DR BioGRID; 33177; 317.
DR DIP; DIP-1351N; -.
DR IntAct; P22149; 63.
DR MINT; P22149; -.
DR STRING; 4932.YGL071W; -.
DR iPTMnet; P22149; -.
DR MaxQB; P22149; -.
DR PaxDb; P22149; -.
DR PRIDE; P22149; -.
DR EnsemblFungi; YGL071W_mRNA; YGL071W; YGL071W.
DR GeneID; 852809; -.
DR KEGG; sce:YGL071W; -.
DR SGD; S000003039; AFT1.
DR VEuPathDB; FungiDB:YGL071W; -.
DR eggNOG; KOG4818; Eukaryota.
DR GeneTree; ENSGT00940000176694; -.
DR HOGENOM; CLU_025287_0_0_1; -.
DR InParanoid; P22149; -.
DR OMA; EHNEYIL; -.
DR BioCyc; YEAST:G3O-30574-MON; -.
DR PRO; PR:P22149; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P22149; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0043515; F:kinetochore binding; IDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032048; P:cardiolipin metabolic process; IMP:SGD.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR GO; GO:0034087; P:establishment of mitotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0034758; P:positive regulation of iron ion transport; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0036086; P:positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation; IMP:SGD.
DR GO; GO:1990641; P:response to iron ion starvation; IMP:SGD.
DR InterPro; IPR014842; AFT.
DR Pfam; PF08731; AFT; 1.
PE 1: Evidence at protein level;
KW Activator; Iron; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..690
FT /note="Iron-regulated transcriptional activator AFT1"
FT /id="PRO_0000064487"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 335..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 612..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..176
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 616..639
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..655
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 291
FT /note="C -> F (in allele AFT1-1UP; which is constitutively
FT activated)"
FT CONFLICT 8
FT /note="D -> H (in Ref. 2; CAA54586)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="I -> S (in Ref. 2; CAA54586)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="A -> T (in Ref. 2; CAA54586)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="D -> N (in Ref. 2; CAA54586)"
FT /evidence="ECO:0000305"
FT CONFLICT 329
FT /note="S -> L (in Ref. 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="S -> L (in Ref. 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 416
FT /note="D -> G (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 468
FT /note="N -> T (in Ref. 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 507
FT /note="N -> S (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 538
FT /note="S -> F (in Ref. 2; CAA54586)"
FT /evidence="ECO:0000305"
FT CONFLICT 538
FT /note="S -> L (in Ref. 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 568
FT /note="S -> P (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 579
FT /note="S -> T (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 644
FT /note="H -> I (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
FT CONFLICT 649
FT /note="H -> L (in Ref. 2; CAA54586 and 7; CAA37215)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 690 AA; 77683 MW; 38641F26B76FCBDD CRC64;
MEGFNPADIE HASPINSSDS HSSSFVYALP KSASEYVVNH NEGRASASGN PAAVPSPIMT
LNLKSTHSLN IDQHVHTSTS PTETIGHIHH VEKLNQNNLI HLDPVPNFED KSDIKPWLQK
IFYPQGIELV IERSDAFKVV FKCKAAKRGR NARRKRKDKP KGQDHEDEKS KINDDELEYA
SPSNATVTNG PQTSPDQTSS IKPKKKRCVS RFNNCPFRVR ATYSLKRKRW SIVVMDNNHS
HQLKFNPDSE EYKKFKEKLR KDNDVDAIKK FDELEYRTLA NLPIPTATIP CDCGLTNEIQ
SFNVVLPTNS NVTSSASSST VSSISLDSSN ASKRPCLPSV NNTGSINTNN VRKPKSQCKN
KDTLLKRTTM QNFLTTKSRL RKTGTPTSSQ HSSTAFSGYI DDPFNLNEIL PLPASDFKLN
TVTNLNEIDF TNIFTKSPHP HSGSTHPRQV FDQLDDCSSI LFSPLTTNTN NEFEGESDDF
VHSPYLNSEA DFSQILSSAP PVHHDPNETH QENQDIIDRF ANSSQEHNEY ILQYLTHSDA
ANHNNIGVPN NNSHSLNTQH NVSDLGNSLL RQEALVGSSS TKIFDELKFV QNGPHGSQHP
IDFQHVDHRH LSSNEPQVRS HQYGPQQQPP QQLQYHQNQP HDGHNHEQHQ TVQKDMQTHE
SLEIMGNTLL EEFKDIKMVN GELKYVKPED
