EID1_ARATH
ID EID1_ARATH Reviewed; 336 AA.
AC Q8LEA8; O81289; Q0WMI9;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Phytochrome A-associated F-box protein;
DE AltName: Full=Empfindlicher im dunkelroten Licht protein 1;
GN Name=EID1; OrderedLocusNames=At4g02440; ORFNames=T14P8.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=10760243; DOI=10.2307/3871068;
RA Bueche C., Poppe C., Schaefer E., Kretsch T.;
RT "eid1: a new Arabidopsis mutant hypersensitive in phytochrome A-dependent
RT high-irradiance responses.";
RL Plant Cell 12:547-558(2000).
RN [7]
RP SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, INTERACTION WITH SKP1A
RP AND SKP1B, DOMAIN, AND MUTAGENESIS OF PRO-10; GLY-101 AND 197-ARG-LYS-198.
RX PubMed=11316788; DOI=10.1101/gad.197201;
RA Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T.;
RT "EID1, an F-box protein involved in phytochrome A-specific light
RT signaling.";
RL Genes Dev. 15:939-944(2001).
RN [8]
RP INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. Negative regulator of the
CC phyA signaling pathway that shifts the responsiveness of the phyA
CC signaling system associated with hypocotyl elongation from red to far-
CC red wavelength. {ECO:0000269|PubMed:10760243}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Probable component of an E3 ubiquitin ligase SCF complex.
CC Interacts with SKP1A/ASK1 and SKP1B/ASK2. {ECO:0000269|PubMed:11316788,
CC ECO:0000269|PubMed:12795696}.
CC -!- INTERACTION:
CC Q8LEA8; O49484: ASK11; NbExp=3; IntAct=EBI-687388, EBI-401185;
CC Q8LEA8; O81057: ASK14; NbExp=3; IntAct=EBI-687388, EBI-401221;
CC Q8LEA8; Q9LNT9: ASK4; NbExp=4; IntAct=EBI-687388, EBI-604085;
CC Q8LEA8; Q39255: SKP1A; NbExp=4; IntAct=EBI-687388, EBI-532357;
CC Q8LEA8; Q9FHW7: SKP1B; NbExp=4; IntAct=EBI-687388, EBI-604076;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11316788}.
CC -!- DOMAIN: The F-box domain is required for the interaction with SKP1A and
CC SKP1B. {ECO:0000269|PubMed:11316788}.
CC -!- MISCELLANEOUS: 'Empfindlicher im dunkelroten licht' means sensitive to
CC far-red light in German.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF069298; AAC19270.1; -; Genomic_DNA.
DR EMBL; AL161494; CAB80737.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82172.1; -; Genomic_DNA.
DR EMBL; BT002038; AAN72049.1; -; mRNA.
DR EMBL; BT008731; AAP42744.1; -; mRNA.
DR EMBL; AK229833; BAF01662.1; -; mRNA.
DR EMBL; AY085530; AAM62754.1; -; mRNA.
DR PIR; T01306; T01306.
DR RefSeq; NP_192153.1; NM_116477.1.
DR AlphaFoldDB; Q8LEA8; -.
DR BioGRID; 13299; 11.
DR IntAct; Q8LEA8; 9.
DR STRING; 3702.AT4G02440.1; -.
DR PaxDb; Q8LEA8; -.
DR PRIDE; Q8LEA8; -.
DR ProteomicsDB; 220758; -.
DR EnsemblPlants; AT4G02440.1; AT4G02440.1; AT4G02440.
DR GeneID; 828008; -.
DR Gramene; AT4G02440.1; AT4G02440.1; AT4G02440.
DR KEGG; ath:AT4G02440; -.
DR Araport; AT4G02440; -.
DR TAIR; locus:2133259; AT4G02440.
DR eggNOG; ENOG502QQ62; Eukaryota.
DR HOGENOM; CLU_049819_0_0_1; -.
DR InParanoid; Q8LEA8; -.
DR OMA; GCIHVEE; -.
DR OrthoDB; 961109at2759; -.
DR PhylomeDB; Q8LEA8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8LEA8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LEA8; baseline and differential.
DR Genevisible; Q8LEA8; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:TAIR.
DR GO; GO:0009585; P:red, far-red light phototransduction; IMP:TAIR.
DR GO; GO:0010099; P:regulation of photomorphogenesis; IMP:TAIR.
DR InterPro; IPR040267; EID1-like.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR PANTHER; PTHR31348; PTHR31348; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phytochrome signaling pathway; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..336
FT /note="Phytochrome A-associated F-box protein"
FT /id="PRO_0000119961"
FT DOMAIN 3..55
FT /note="F-box"
FT MOTIF 197..201
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:11316788"
FT MUTAGEN 10
FT /note="P->A: Disrupts interaction with SKP1A and SKP1B."
FT /evidence="ECO:0000269|PubMed:11316788"
FT MUTAGEN 101
FT /note="G->C: In eid1-2; enhances sensitivity to red and
FT far-red light."
FT /evidence="ECO:0000269|PubMed:11316788"
FT MUTAGEN 197..198
FT /note="RK->SE: Cytoplasmic localization instead of
FT nuclear."
FT /evidence="ECO:0000269|PubMed:11316788"
FT CONFLICT 16
FT /note="N -> K (in Ref. 5; AAM62754)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="A -> AA (in Ref. 5; AAM62754)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 336 AA; 37398 MW; 0E4231CCF243487C CRC64;
MAESVFSCIP EDVVFNIFFK LQDDPRNWAR LACVCTKFSS IVRNVCCKTQ CYSAIPTVIS
DLLPLPPSAA ASASSSTAAD SSLTPPGGWA SLYKLAVCCP GLFHAGILLE NSDFGLEREL
GPDQNLDPKP TTTDLALNDE EVSKPVGSGL ETTSFWSLYD DLYTDTIPAP PPEDSIDDQE
EEIETSEIRP GRDLPVRKRR KICRSLGSHL ASGGWNLSRE QGNKLLASRF RGDCLYICNW
PGCIHVEEKR NYMLFRGVFK DFKRSRVWRT INDGNRSKTS GLKCAFCLCD ETWDLHSSFC
LRRVFGFHDD GEPVVRAYVC ENGHVSGAWT ALPLYT
