EIF1A_MOUSE
ID EIF1A_MOUSE Reviewed; 170 AA.
AC Q3THJ3; Q8K1C2; Q9D8F9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable RNA-binding protein EIF1AD;
DE AltName: Full=Eukaryotic translation initiation factor 1A domain-containing protein;
GN Name=Eif1ad;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=BALB/cJ, and C57BL/6J;
RC TISSUE=Bone marrow, Kidney, Small intestine, and Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131; SER-136 AND SER-159, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role into cellular response to oxidative stress.
CC Decreases cell proliferation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GAPDH and STAT1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EIF1AD family. {ECO:0000305}.
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DR EMBL; AK008070; BAB25440.1; -; mRNA.
DR EMBL; AK153194; BAE31794.1; -; mRNA.
DR EMBL; AK158977; BAE34752.1; -; mRNA.
DR EMBL; AK168598; BAE40465.1; -; mRNA.
DR EMBL; AK168102; BAE40073.1; -; mRNA.
DR EMBL; AK168253; BAE40203.1; -; mRNA.
DR EMBL; BC024640; AAH24640.1; -; mRNA.
DR CCDS; CCDS29459.1; -.
DR RefSeq; NP_081512.1; NM_027236.2.
DR AlphaFoldDB; Q3THJ3; -.
DR SMR; Q3THJ3; -.
DR IntAct; Q3THJ3; 2.
DR MINT; Q3THJ3; -.
DR STRING; 10090.ENSMUSP00000025759; -.
DR iPTMnet; Q3THJ3; -.
DR PhosphoSitePlus; Q3THJ3; -.
DR EPD; Q3THJ3; -.
DR jPOST; Q3THJ3; -.
DR MaxQB; Q3THJ3; -.
DR PaxDb; Q3THJ3; -.
DR PeptideAtlas; Q3THJ3; -.
DR PRIDE; Q3THJ3; -.
DR ProteomicsDB; 277845; -.
DR Antibodypedia; 44351; 77 antibodies from 13 providers.
DR DNASU; 69860; -.
DR Ensembl; ENSMUST00000025759; ENSMUSP00000025759; ENSMUSG00000024841.
DR GeneID; 69860; -.
DR KEGG; mmu:69860; -.
DR UCSC; uc008gct.1; mouse.
DR CTD; 84285; -.
DR MGI; MGI:1917110; Eif1ad.
DR VEuPathDB; HostDB:ENSMUSG00000024841; -.
DR eggNOG; KOG2925; Eukaryota.
DR GeneTree; ENSGT00390000011180; -.
DR HOGENOM; CLU_106477_2_1_1; -.
DR InParanoid; Q3THJ3; -.
DR OMA; YLVSMPR; -.
DR OrthoDB; 1431625at2759; -.
DR PhylomeDB; Q3THJ3; -.
DR TreeFam; TF314439; -.
DR BioGRID-ORCS; 69860; 27 hits in 71 CRISPR screens.
DR ChiTaRS; Eif1ad; mouse.
DR PRO; PR:Q3THJ3; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q3THJ3; protein.
DR Bgee; ENSMUSG00000024841; Expressed in ear vesicle and 225 other tissues.
DR Genevisible; Q3THJ3; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:InterPro.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR039294; EIF1AD.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006196; RNA-binding_domain_S1_IF1.
DR InterPro; IPR001253; TIF_eIF-1A.
DR PANTHER; PTHR21641; PTHR21641; 1.
DR Pfam; PF01176; eIF-1a; 1.
DR SMART; SM00652; eIF1a; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50832; S1_IF1_TYPE; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..170
FT /note="Probable RNA-binding protein EIF1AD"
FT /id="PRO_0000314153"
FT DOMAIN 5..89
FT /note="S1-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00181"
FT REGION 112..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 6..12
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 56..65
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 112..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 33
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8N9N8"
FT MOD_RES 131
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 135
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5RKI6"
FT MOD_RES 136
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 137
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5RKI6"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N9N8"
FT MOD_RES 159
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 74
FT /note="I -> V (in Ref. 1; BAB25440)"
FT /evidence="ECO:0000305"
FT CONFLICT 103
FT /note="P -> R (in Ref. 1; BAE40073/BAE40203)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 170 AA; 19520 MW; DD70717832E439B5 CRC64;
MSQASKRKHV VQEVLGEHMV PSDHQQIVKV LRTPGNNLHE VETAQGQRFL VSMPSKYRKN
IWIKRGDFLI VDPIEEGEKV KAEISFVLCK NHVRSLQKEG HWPEAFSEVA EKQNNMNRES
QPELPAEPQL SGEGSSSEDD SDLFVNTNHR QYHESEEESE EDEEEEEEAA
