EIF2A_DROME
ID EIF2A_DROME Reviewed; 638 AA.
AC Q9VNX8;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Eukaryotic translation initiation factor 2A;
DE Short=eIF-2A;
GN ORFNames=CG7414;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-463, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Functions in the early steps of protein synthesis of a small
CC number of specific mRNAs. Acts by directing the binding of methionyl-
CC tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it
CC binds methionyl-tRNAi to 40S subunits in a codon-dependent manner,
CC whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a
CC GTP-dependent manner. {ECO:0000250|UniProtKB:Q9BY44}.
CC -!- SIMILARITY: Belongs to the WD repeat EIF2A family. {ECO:0000305}.
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DR EMBL; AE014296; AAF51786.1; -; Genomic_DNA.
DR EMBL; AY051717; AAK93141.1; -; mRNA.
DR RefSeq; NP_001137996.2; NM_001144524.3.
DR RefSeq; NP_649365.1; NM_141108.2.
DR AlphaFoldDB; Q9VNX8; -.
DR SMR; Q9VNX8; -.
DR BioGRID; 65671; 4.
DR IntAct; Q9VNX8; 1.
DR STRING; 7227.FBpp0078095; -.
DR iPTMnet; Q9VNX8; -.
DR PaxDb; Q9VNX8; -.
DR PRIDE; Q9VNX8; -.
DR DNASU; 40431; -.
DR EnsemblMetazoa; FBtr0078441; FBpp0078095; FBgn0037135.
DR EnsemblMetazoa; FBtr0303246; FBpp0292338; FBgn0037135.
DR GeneID; 40431; -.
DR KEGG; dme:Dmel_CG7414; -.
DR UCSC; CG7414-RA; d. melanogaster.
DR FlyBase; FBgn0037135; CG7414.
DR VEuPathDB; VectorBase:FBgn0037135; -.
DR eggNOG; KOG2315; Eukaryota.
DR GeneTree; ENSGT00730000111053; -.
DR HOGENOM; CLU_013809_1_0_1; -.
DR InParanoid; Q9VNX8; -.
DR OMA; LITWQRP; -.
DR PhylomeDB; Q9VNX8; -.
DR BioGRID-ORCS; 40431; 0 hits in 1 CRISPR screen.
DR ChiTaRS; CG7414; fly.
DR GenomeRNAi; 40431; -.
DR PRO; PR:Q9VNX8; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0037135; Expressed in capitellum (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q9VNX8; baseline and differential.
DR Genevisible; Q9VNX8; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:FlyBase.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; ISS:FlyBase.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; ISS:FlyBase.
DR GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR GO; GO:0006413; P:translational initiation; TAS:FlyBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR011387; TIF2A.
DR InterPro; IPR013979; TIF_beta_prop-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR13227; PTHR13227; 1.
DR Pfam; PF08662; eIF2A; 1.
DR PIRSF; PIRSF017222; eIF2A; 1.
PE 1: Evidence at protein level;
KW Initiation factor; Phosphoprotein; Protein biosynthesis;
KW Reference proteome; Repeat; Translation regulation; WD repeat.
FT CHAIN 1..638
FT /note="Eukaryotic translation initiation factor 2A"
FT /id="PRO_0000286082"
FT REPEAT 287..329
FT /note="WD 1"
FT REPEAT 331..370
FT /note="WD 2"
FT REGION 441..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 463
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 638 AA; 70071 MW; 5F68FACE250456DD CRC64;
MAAAADLVVP TLAIRSSVAV ELWSSAGSKQ PFEHKPHLPR EETKSSRSIC FSSNGRYFAY
SNGQEVKVLQ ASRDSAASWP VICVLPRPKA FYLQFSPRGS YLCTWEHYAI TKDRPEGSPN
LLVYEVATGV EVFAIVQKNQ ADWQPSWSAD ESIFALVVGG EALFYDLGEG AEKGFATTSR
KIGGSRGGML SLGPGNCPPF LAFYTPGAKG APSMCKLYKY PALGQNQTVA CKSFFQADRV
EMLWNKRGSG LLLLTSTEVD KSGASYYGNQ AVHFMATKGD TCSVPLSKEG PVHCVKWSPK
ATEFVVVYGF MPSKAALYNL KCDVVFDFGE GPRNCAYFNP FGNLIALAGF GNLPGAVEVW
DVSKREKLAN LKCADTTVFE WHPNGEWFVT ATTAPRLRIS NGFKVYHYSG ALLHETMWPQ
GQELLGIEWQ QFADKTFSEP KITKAKHEGI KSSQPEASKK AYTPPHLRLL KEGKNPEKYL
PQPSIPGLAP TAAAGGVNGN KRNNKNKQRS ARKDVNVVNG GDADSAPTEV VDQAAPVARQ
SPVFVAAEER KPQHTPRQKY QPDNAADQPP NSYGQGHPNG QHISDKERKI RSVAKKLSDI
KKLKVRRSQG ENLELNQLNK IKMEAQYLDE LKALKQSA
