EIF2A_SCHPO
ID EIF2A_SCHPO Reviewed; 576 AA.
AC O74965;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Eukaryotic translation initiation factor 2A;
DE Short=eIF-2A;
GN ORFNames=SPBC4B4.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Functions in the early steps of protein synthesis of a small
CC number of specific mRNAs. Acts by directing the binding of methionyl-
CC tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it
CC binds methionyl-tRNAi to 40S subunits in a codon-dependent manner,
CC whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a
CC GTP-dependent manner. {ECO:0000250|UniProtKB:Q9BY44}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat EIF2A family. {ECO:0000305}.
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DR EMBL; CU329671; CAA19284.1; -; Genomic_DNA.
DR PIR; T40476; T40476.
DR RefSeq; NP_596421.1; NM_001022340.2.
DR PDB; 3WJ9; X-ray; 2.51 A; A/B=2-412.
DR PDBsum; 3WJ9; -.
DR AlphaFoldDB; O74965; -.
DR SMR; O74965; -.
DR BioGRID; 277515; 175.
DR STRING; 4896.SPBC4B4.04.1; -.
DR MaxQB; O74965; -.
DR PaxDb; O74965; -.
DR PRIDE; O74965; -.
DR EnsemblFungi; SPBC4B4.04.1; SPBC4B4.04.1:pep; SPBC4B4.04.
DR GeneID; 2540999; -.
DR KEGG; spo:SPBC4B4.04; -.
DR PomBase; SPBC4B4.04; -.
DR VEuPathDB; FungiDB:SPBC4B4.04; -.
DR eggNOG; KOG2315; Eukaryota.
DR HOGENOM; CLU_013809_0_1_1; -.
DR InParanoid; O74965; -.
DR OMA; LITWQRP; -.
DR PhylomeDB; O74965; -.
DR PRO; PR:O74965; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; ISS:PomBase.
DR GO; GO:0003743; F:translation initiation factor activity; ISO:PomBase.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; ISO:PomBase.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR011387; TIF2A.
DR InterPro; IPR013979; TIF_beta_prop-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR13227; PTHR13227; 1.
DR Pfam; PF08662; eIF2A; 1.
DR PIRSF; PIRSF017222; eIF2A; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Initiation factor; Protein biosynthesis;
KW Reference proteome; Repeat; Translation regulation; WD repeat.
FT CHAIN 1..576
FT /note="Eukaryotic translation initiation factor 2A"
FT /id="PRO_0000286083"
FT REPEAT 71..119
FT /note="WD 1"
FT REPEAT 266..307
FT /note="WD 2"
FT REPEAT 308..349
FT /note="WD 3"
FT REPEAT 351..396
FT /note="WD 4"
FT REGION 422..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 475..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 475..489
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 6..13
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 15..19
FT /evidence="ECO:0007829|PDB:3WJ9"
FT HELIX 20..22
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 46..55
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:3WJ9"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 67..71
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 75..81
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 85..92
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 106..113
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 116..120
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 138..143
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 146..152
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 160..162
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 170..173
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 176..178
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 180..185
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 193..201
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 203..214
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 228..233
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 242..247
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 267..269
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 271..276
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 283..297
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 299..301
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 303..311
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 314..317
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 321..327
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 330..333
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 335..340
FT /evidence="ECO:0007829|PDB:3WJ9"
FT TURN 341..344
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 345..352
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 358..361
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 365..372
FT /evidence="ECO:0007829|PDB:3WJ9"
FT TURN 374..376
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 382..390
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 392..397
FT /evidence="ECO:0007829|PDB:3WJ9"
FT STRAND 399..406
FT /evidence="ECO:0007829|PDB:3WJ9"
SQ SEQUENCE 576 AA; 63497 MW; 6702ADCCAF5A2A62 CRC64;
MSQKSQFAYR SSKSIGLVNA SENYASPPKF EAISEPARNA CYSPNGKLFA YATATQVVIN
DTESGAKLTQ LPAANTYELG FSPLGKYLST WERPGKEADG TPKQNMKVWN TETGQLVFSF
VQRNQTGWNL QYTCDESLAA RLVTNEVHFY ETGNMSKGPI AKLRVEGISD FALSPGQNHA
VAVFIPEKKG APASVRTYSI PNFNSPLSQK TFFKADKVQF KWNALGTSLL VLTQTEVDKS
NKNYYGETNL YLLGITGQFD CRVDLDREGP IHDVCWNADS KEFGIVYGYM PAKTAIFDNR
ANVVSIIPPA PRNTLIFSPN SRYILLAGFG NLQGSIDIFD AANNMKKITT VEAANCTYCE
FSPDSQFLLT AVTSPRLRVD NSIKIWHITG APMFYEEFNE LYQAFWRPRP LNPTLLQNAL
TSASLPSPPT PHASASKLAA KPSVKPAGAY RPPGARGQNS TFSYRREEID VMSSGSANKH
VNSSRQRVVP GATPVIDGNK KNNKKTKPAV KQVAQPTAEV SDEKKIRSLC KKLRAIDDLK
SRLNNNEKLE ATQVKKIESE GKVLAELKAL GWTPDA
