AFTA_MYCS2
ID AFTA_MYCS2 Reviewed; 624 AA.
AC A0R611; I7GGD8;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Galactan 5-O-arabinofuranosyltransferase {ECO:0000250|UniProtKB:P9WN03};
DE EC=2.4.2.46 {ECO:0000250|UniProtKB:P9WN03};
DE AltName: Full=Arabinofuranosyltransferase AftA {ECO:0000250|UniProtKB:P9WN03};
GN Name=aftA {ECO:0000250|UniProtKB:P9WN03};
GN OrderedLocusNames=MSMEG_6386, MSMEI_6218;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP DISRUPTION PHENOTYPE, AND PATHWAY.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18556798; DOI=10.1128/jb.00028-08;
RA Shi L., Zhou R., Liu Z., Lowary T.L., Seeberger P.H., Stocker B.L.,
RA Crick D.C., Khoo K.H., Chatterjee D.;
RT "Transfer of the first arabinofuranose residue to galactan is essential for
RT Mycobacterium smegmatis viability.";
RL J. Bacteriol. 190:5248-5255(2008).
CC -!- FUNCTION: Involved in the biosynthesis of the arabinogalactan (AG)
CC region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an
CC essential component of the mycobacterial cell wall. Catalyzes the
CC addition of the first key arabinofuranosyl (Araf) residue from the
CC sugar donor decaprenyl-phospho-arabinose (DPA) on the C-5 of a 6-linked
CC galactofuranosyl (Galf) of the galactan domain, thus 'priming' the
CC galactan for further elaboration by other arabinofuranosyltransferases.
CC It is not able to add an Araf residue to a terminal Galf.
CC {ECO:0000250|UniProtKB:P9WN03}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Adds an alpha-D-arabinofuranosyl group from trans,octacis-
CC decaprenylphospho-beta-D-arabinofuranose at the 5-O-position of the
CC eighth, tenth and twelfth galactofuranose unit of the galactofuranan
CC chain of [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-
CC (1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-
CC (1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-
CC diphospho-trans,octacis-decaprenol.; EC=2.4.2.46;
CC Evidence={ECO:0000250|UniProtKB:P9WN03};
CC -!- PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis.
CC {ECO:0000305|PubMed:18556798}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P9WN03};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:P9WN03}.
CC -!- DISRUPTION PHENOTYPE: Allelic exchange at the chromosomal MSMEG_6386
CC locus of M.smegmatis can only be achieved in the presence of a rescue
CC plasmid carrying a functional copy of MSMEG_6386 or Rv3792, strongly
CC suggesting that this gene is essential. {ECO:0000269|PubMed:18556798}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 85 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFP42644.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK70967.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP42644.1; ALT_INIT; Genomic_DNA.
DR RefSeq; YP_890599.1; NC_008596.1.
DR AlphaFoldDB; A0R611; -.
DR STRING; 246196.MSMEI_6218; -.
DR CAZy; GT85; Glycosyltransferase Family 85.
DR PRIDE; A0R611; -.
DR EnsemblBacteria; ABK70967; ABK70967; MSMEG_6386.
DR EnsemblBacteria; AFP42644; AFP42644; MSMEI_6218.
DR KEGG; msg:MSMEI_6218; -.
DR KEGG; msm:MSMEG_6386; -.
DR PATRIC; fig|246196.19.peg.6213; -.
DR eggNOG; ENOG502ZB59; Bacteria.
DR OMA; YPAGWFW; -.
DR UniPathway; UPA00963; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016740; F:transferase activity; IDA:UniProtKB.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0044038; P:cell wall macromolecule biosynthetic process; IDA:UniProtKB.
DR GO; GO:0071555; P:cell wall organization; IDA:UniProtKB.
DR InterPro; IPR020959; ArabinofuranosylTrfase_AftA_C.
DR InterPro; IPR020963; ArabinofuranosylTrfase_AftA_N.
DR Pfam; PF12249; AftA_C; 1.
DR Pfam; PF12250; AftA_N; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..624
FT /note="Galactan 5-O-arabinofuranosyltransferase"
FT /id="PRO_0000395359"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 624 AA; 67201 MW; 8D5324A7195CB358 CRC64;
MAARVLGQMV LAVLMASAVA GVAIAAIARV EWPAYNTSNQ LHALTTVGQF GCLAGLFAAG
LLWRRGRRTL ARLGALAFIS AFSVVTLAMP LGATKLYLFG VSVDQQFRTE YLTRLTDTAG
LHDMTYIGLP PFYPAGWFWL GGRIAAATGT PAWEMFKPWS IVSITIAVAL ALVLWAAMIR
FEYALVATAA STAAMLAYAS TEPYAAIITV LMPPVFVLAW AGLRARTRGG GWAAIVGVGI
FLGVAALFYT LLLVYAAFTL TIMALCVAVA RRHIDPLLRL AVIAVISGAI ALLTWAPYLL
AAMRGEPADS GTAQHYLPDA GAELHFPMFS LTLHGALCML GTVWLVVRAR TSTRAGALAV
AVVAVYAWSL LSMLTTLAGT TLLSFRLQPT LTVLLTTAGA FGFIEATLAI AHRYRPETSR
RVVAAATAVG AIGAVTFSQD IPDVLRPDIN VAYTDTDGTG QRADRRPPGA ERYYREIDAK
ILEVTGVPRN QTVVLTADYS FLSFYPYYGF QGLTSHYANP LAQFDKRAAA IEGWATLGSA
DDFVAALDEL PWEPPTVFLM RHGANDTYTL RLASDVYPNQ PNVRRYHVEL DSAVFDDPRF
EVSDHGPFVL AIRKPGGKPE TDGH
