EIF3B_ASHGO
ID EIF3B_ASHGO Reviewed; 736 AA.
AC Q758X9;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001};
DE Short=eIF3b {ECO:0000255|HAMAP-Rule:MF_03001};
DE AltName: Full=Eukaryotic translation initiation factor 3 90 kDa subunit homolog {ECO:0000255|HAMAP-Rule:MF_03001};
DE Short=eIF3 p90 {ECO:0000255|HAMAP-Rule:MF_03001};
DE AltName: Full=Translation initiation factor eIF3 p90 subunit homolog;
GN Name=PRT1 {ECO:0000255|HAMAP-Rule:MF_03001}; OrderedLocusNames=ADR399C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: RNA-binding component of the eukaryotic translation
CC initiation factor 3 (eIF-3) complex, which is involved in protein
CC synthesis of a specialized repertoire of mRNAs and, together with other
CC initiation factors, stimulates binding of mRNA and methionyl-tRNAi to
CC the 40S ribosome. The eIF-3 complex specifically targets and initiates
CC translation of a subset of mRNAs involved in cell proliferation.
CC {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex. {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SIMILARITY: Belongs to the eIF-3 subunit B family. {ECO:0000255|HAMAP-
CC Rule:MF_03001}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE016817; AAS52318.1; -; Genomic_DNA.
DR RefSeq; NP_984494.1; NM_209847.1.
DR AlphaFoldDB; Q758X9; -.
DR SMR; Q758X9; -.
DR STRING; 33169.AAS52318; -.
DR EnsemblFungi; AAS52318; AAS52318; AGOS_ADR399C.
DR GeneID; 4620659; -.
DR KEGG; ago:AGOS_ADR399C; -.
DR eggNOG; KOG2314; Eukaryota.
DR HOGENOM; CLU_011152_4_0_1; -.
DR InParanoid; Q758X9; -.
DR OMA; NVADCKI; -.
DR Proteomes; UP000000591; Chromosome IV.
DR GO; GO:0010494; C:cytoplasmic stress granule; IEA:EnsemblFungi.
DR GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005852; C:eukaryotic translation initiation factor 3 complex; IBA:GO_Central.
DR GO; GO:0071540; C:eukaryotic translation initiation factor 3 complex, eIF3e; IEA:EnsemblFungi.
DR GO; GO:0071541; C:eukaryotic translation initiation factor 3 complex, eIF3m; IEA:EnsemblFungi.
DR GO; GO:0043614; C:multi-eIF complex; IEA:EnsemblFungi.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0031369; F:translation initiation factor binding; IEA:InterPro.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR CDD; cd12278; RRM_eIF3B; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR HAMAP; MF_03001; eIF3b; 1.
DR InterPro; IPR011400; EIF3B.
DR InterPro; IPR034363; eIF3B_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR013979; TIF_beta_prop-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR14068; PTHR14068; 1.
DR Pfam; PF08662; eIF2A; 1.
DR Pfam; PF00076; RRM_1; 1.
DR PIRSF; PIRSF036424; eIF3b; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Initiation factor; Protein biosynthesis; Reference proteome;
KW Repeat; RNA-binding; WD repeat.
FT CHAIN 1..736
FT /note="Eukaryotic translation initiation factor 3 subunit
FT B"
FT /id="PRO_0000363806"
FT DOMAIN 37..120
FT /note="RRM"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
FT REPEAT 140..182
FT /note="WD 1"
FT REPEAT 186..224
FT /note="WD 2"
FT REPEAT 226..244
FT /note="WD 3"
FT REPEAT 245..284
FT /note="WD 4"
FT REPEAT 288..328
FT /note="WD 5"
FT REPEAT 332..375
FT /note="WD 6"
FT REPEAT 443..483
FT /note="WD 7"
FT REPEAT 511..557
FT /note="WD 8"
FT REPEAT 566..604
FT /note="WD 9"
FT REPEAT 616..662
FT /note="WD 10"
FT REGION 1..219
FT /note="Sufficient for interaction with PIC8"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
FT REGION 1..94
FT /note="Sufficient for interaction with HCR1 and TIF32"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
SQ SEQUENCE 736 AA; 84631 MW; DA3B4FB13A16D9C2 CRC64;
MAAVFDDIRL EDIPVDDVDM QDLEETYAVE RSIEFDRYVV VDGAPVAPEA KVGALQKVLT
KLFSQAGSVV DMDVPVEEGR TKGHLFIEFE DAGAARRAIK MFNGKKLDVK HRLWVNGLDD
MERYGRPDFS TEYREPVVPE FEATEYPRSW LQDETGRDQF VLQKGEMTAV FWNRNNLQPE
NVVEPRRNWS NSILNFSPHG TYLFSFHDQG IASWGGPQFK RLRRFAHPDV KAISMSSTEK
YLVTFSSEPL EVSDEPNEAC PFGPESRGHQ LCIWDVATGV CVKTFALPPQ QQLQWPMVKW
SFDDKFCARL GPGAIAVYET EKNFQLLGGK VMKIEDVQDF SFAPKGIKLA SNRPNDPPST
VMVYWTPESN NQSCKAVLIE LPNRRVLRTI NLVQVTDVSF HWQNQAEFLC VQVDRHTKSR
KTIFTNMEIC SLTAREFPFE KVEIKDRCMR FAWEPNSDRF VIISRSENVN DNPAIAKNVV
SFYAPEKKVD KKGVIIDKEL SIFKKWKLVR AIDGKFSNEI TWSPAGRFVC VAAIGKIGSR
NENIDFYDMD YPNTEKIINT ATDVNATLRD VAHINYASAT DYEWDPSGRY LAFWSSAWKH
KAENGYKVFN LAGAIVREEL ITDFNNFFWR PRPDSLLSNS EKKKVRKNLK EWSAHFEEQD
AMEADSATRE LILKRRNWLD EWSKYREACK QTLSESGLSI CDCVELSTKD EDCELVEEIR
ETVVEESTEE VPFFEE
