EIF3B_KLULA
ID EIF3B_KLULA Reviewed; 732 AA.
AC Q6CY34;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001};
DE Short=eIF3b {ECO:0000255|HAMAP-Rule:MF_03001};
DE AltName: Full=Eukaryotic translation initiation factor 3 90 kDa subunit homolog {ECO:0000255|HAMAP-Rule:MF_03001};
DE Short=eIF3 p90 {ECO:0000255|HAMAP-Rule:MF_03001};
DE AltName: Full=Translation initiation factor eIF3 p90 subunit homolog;
GN Name=PRT1 {ECO:0000255|HAMAP-Rule:MF_03001};
GN OrderedLocusNames=KLLA0A03531g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: RNA-binding component of the eukaryotic translation
CC initiation factor 3 (eIF-3) complex, which is involved in protein
CC synthesis of a specialized repertoire of mRNAs and, together with other
CC initiation factors, stimulates binding of mRNA and methionyl-tRNAi to
CC the 40S ribosome. The eIF-3 complex specifically targets and initiates
CC translation of a subset of mRNAs involved in cell proliferation.
CC {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex. {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03001}.
CC -!- SIMILARITY: Belongs to the eIF-3 subunit B family. {ECO:0000255|HAMAP-
CC Rule:MF_03001}.
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DR EMBL; CR382121; CAH02743.1; -; Genomic_DNA.
DR RefSeq; XP_451155.1; XM_451155.1.
DR AlphaFoldDB; Q6CY34; -.
DR SMR; Q6CY34; -.
DR STRING; 28985.XP_451155.1; -.
DR EnsemblFungi; CAH02743; CAH02743; KLLA0_A03531g.
DR GeneID; 2896595; -.
DR KEGG; kla:KLLA0_A03531g; -.
DR eggNOG; KOG2314; Eukaryota.
DR HOGENOM; CLU_011152_4_0_1; -.
DR InParanoid; Q6CY34; -.
DR OMA; NVADCKI; -.
DR Proteomes; UP000000598; Chromosome A.
DR GO; GO:0010494; C:cytoplasmic stress granule; IEA:EnsemblFungi.
DR GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0071540; C:eukaryotic translation initiation factor 3 complex, eIF3e; IEA:EnsemblFungi.
DR GO; GO:0071541; C:eukaryotic translation initiation factor 3 complex, eIF3m; IEA:EnsemblFungi.
DR GO; GO:0043614; C:multi-eIF complex; IEA:EnsemblFungi.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0031369; F:translation initiation factor binding; IEA:InterPro.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR CDD; cd12278; RRM_eIF3B; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR HAMAP; MF_03001; eIF3b; 1.
DR InterPro; IPR011400; EIF3B.
DR InterPro; IPR034363; eIF3B_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR013979; TIF_beta_prop-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR14068; PTHR14068; 1.
DR Pfam; PF08662; eIF2A; 1.
DR Pfam; PF00076; RRM_1; 1.
DR PIRSF; PIRSF036424; eIF3b; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Initiation factor; Protein biosynthesis; Reference proteome;
KW Repeat; RNA-binding; WD repeat.
FT CHAIN 1..732
FT /note="Eukaryotic translation initiation factor 3 subunit
FT B"
FT /id="PRO_0000363820"
FT DOMAIN 37..120
FT /note="RRM"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
FT REPEAT 185..224
FT /note="WD 1"
FT REPEAT 237..280
FT /note="WD 2"
FT REPEAT 439..481
FT /note="WD 3"
FT REPEAT 507..554
FT /note="WD 4"
FT REGION 1..219
FT /note="Sufficient for interaction with PIC8"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
FT REGION 1..94
FT /note="Sufficient for interaction with HCR1 and TIF32"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
SQ SEQUENCE 732 AA; 84187 MW; D7BC192F71D0EBC1 CRC64;
MTTLESLKIE DIPVDDIDFS DLEREFQIQD GGVNFDNFLV VDGAPVAPEA KVPVLEKVLT
KLFSQAGKVL DMQIPVEDGK TKGHLFIEME SVSAAKEAIQ LFNGKKLDAK HRLLVNSLND
MEKYGSDDFE SQSHEPVVPD FAPTDFLRSW LQNQDGRDQF VLQKGDMTRV FWNRLAHQPD
GVGEARKNWS NDVVKFSPKG TYLLSFHDQG VTSWGGPNFD RLKRFFHPDV SRLDVSPTEK
FLITFSMNPI KPGEDTPFGP ESEGHQICVW DLATGFLMKT FGIPPNAKLQ WPLIRFSYDD
KYCGRLGPNA LAIYDIENNF QLLDGKLHKV EGIQDFSFAP KGVQLTYNRR KSDPTTLLAY
WTPETNNQSC KAFLMTLPNK RIVKTVNLVQ VSNVSIHWHE QADFVCFQVD RHTKSKKTFF
TNLEICKLNE SEIPVEKIEM KDRVLELAWE PKGTRFVTIS KMDNGGEENP MYPKNFVKFF
APEKKDNKDK DLAVLPDQLK WKLVKTVDQQ FANCISWSPA GRFVAVCTIV NGKEIKKASL
DFYDFDFTGE KTLNEVQDVK ASLQAVAHID NQFFTDLEWD SSGRFLTAWS SYSKHKLENG
YTIYNCCGEA VRKEIVDQFR NFVWRPRPES LLSNAEKKKA RKNLKQWSVK FEEQDAMESD
SALRDLILKR RAELSAWVSY REQSKERLES EDSYTIFDNF ESDKADETQY VTVEEVKEEI
LEETQEEVES FE
