ID   EIF3B_TOBAC             Reviewed;         719 AA.
AC   P56821;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001};
DE            Short=eIF3b {ECO:0000255|HAMAP-Rule:MF_03001};
DE   AltName: Full=eIF-3-eta {ECO:0000255|HAMAP-Rule:MF_03001};
DE   AltName: Full=eIF3 p110 {ECO:0000255|HAMAP-Rule:MF_03001};
GN   Name=TIF3B1; Synonyms=PRT1;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bright Yellow 2;
RA   Shen W.H., Gigot C.;
RT   "Characterization of Prt1, a gene encoding for one of the subunits of the
RT   translation initiation factor 3 (eIF3), from Nicotiana tabacum.";
RL   Plant Sci. 143:45-54(1999).
CC   -!- FUNCTION: RNA-binding component of the eukaryotic translation
CC       initiation factor 3 (eIF-3) complex, which is involved in protein
CC       synthesis of a specialized repertoire of mRNAs and, together with other
CC       initiation factors, stimulates binding of mRNA and methionyl-tRNAi to
CC       the 40S ribosome. The eIF-3 complex specifically targets and initiates
CC       translation of a subset of mRNAs involved in cell proliferation.
CC       {ECO:0000255|HAMAP-Rule:MF_03001}.
CC   -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC       (eIF-3) complex. {ECO:0000255|HAMAP-Rule:MF_03001}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03001}.
CC   -!- SIMILARITY: Belongs to the eIF-3 subunit B family. {ECO:0000255|HAMAP-
CC       Rule:MF_03001}.
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DR   EMBL; Y11996; CAA72721.1; -; mRNA.
DR   AlphaFoldDB; P56821; -.
DR   SMR; P56821; -.
DR   STRING; 4097.P56821; -.
DR   iPTMnet; P56821; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005852; C:eukaryotic translation initiation factor 3 complex; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0031369; F:translation initiation factor binding; IEA:InterPro.
DR   GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR   CDD; cd12278; RRM_eIF3B; 1.
DR   Gene3D; 2.130.10.10; -; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   HAMAP; MF_03001; eIF3b; 1.
DR   InterPro; IPR011400; EIF3B.
DR   InterPro; IPR034363; eIF3B_RRM.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR013979; TIF_beta_prop-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR14068; PTHR14068; 1.
DR   Pfam; PF08662; eIF2A; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   PIRSF; PIRSF036424; eIF3b; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Initiation factor; Protein biosynthesis; Reference proteome;
KW   Repeat; RNA-binding; WD repeat.
FT   CHAIN           1..719
FT                   /note="Eukaryotic translation initiation factor 3 subunit
FT                   B"
FT                   /id="PRO_0000123533"
FT   DOMAIN          60..147
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03001"
FT   REPEAT          167..207
FT                   /note="WD 1"
FT   REPEAT          511..553
FT                   /note="WD 2"
FT   REPEAT          555..598
FT                   /note="WD 3"
FT   REGION          675..698
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   719 AA;  82524 MW;  BBFA223B7DA52BB2 CRC64;
     MADVTSMGEI TAIAARIGVD LSQIDLDSIH LPPGEDCGIP SDDDDLMEED PLEFDAGFGN
     ILVVDNLPVV PKEKFEKLEG VVRKIYSQLG VIKEDGLWMP VDPETHKTLG YCFIEYNTPQ
     EAELSKEKTH GYKLDRSHIF AVNMFDDIER FLKVPDEWAP PEIKPYVPGE NLQQWLTDEK
     ARDQFVIRAG NDTEVLWNDA RQLKPELVYK RSFWTESFVQ WSPLGTYLAT VHRQGGAIWG
     GATTSNRLMR YAHPQVKLID FSLAERYLVT YSSHEPSNPR DSHAVVLNIS DVRTGKVMRD
     FQGSADEFAV GGTGGVTGVS WPVFRWSGGK QDKYFARIGK NVISVYETET FSLIDKKSIK
     VENVMDFSWS PADPILALFV PECGNQPARV SLVQIPSKEE LRQKNLFSVS DCKMYWQSNG
     DYLAVKVDRY TKTKKSTYTG FELFRIKERD IPIEVLELDN TNDKIPAFGW GPEGSPICCY
     PSVTTPRPDI RFYSVPVWAL TPGLVSKLTT LKGKQANALY WSPGGRFLIL TGLKGFNGQL
     EFFDVDELET MASAEHFMAT DVEWDPTGRY VATSVTSVHE MENGFNIWSF NGKLLYRILK
     DHFFQYLWRP RPPSFLSKEK EEEIAKNLKR YSKKYEAEDQ DVSLQLSEQD REKRKKLKEE
     WEAWINEWKR LHEEEKMERQ KLRDGEASDE EEEYEAKEVE VEEIINVTEE IIPFEESQQ