ID   AFTR1_ALTAL             Reviewed;         445 AA.
AC   Q96VB4;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Transcription activator AFTR-1 {ECO:0000303|PubMed:12019223};
DE   AltName: Full=ACT-toxin biosynthesis regulator 1 {ECO:0000303|PubMed:12019223};
GN   Name=AFTR-1 {ECO:0000303|PubMed:12019223};
OS   Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Alternaria; Alternaria alternata complex.
OX   NCBI_TaxID=5599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NAF8;
RX   PubMed=12019223; DOI=10.1093/genetics/161.1.59;
RA   Hatta R., Ito K., Hosaki Y., Tanaka T., Tanaka A., Yamamoto M.,
RA   Akimitsu K., Tsuge T.;
RT   "A conditionally dispensable chromosome controls host-specific
RT   pathogenicity in the fungal plant pathogen Alternaria alternata.";
RL   Genetics 161:59-70(2002).
RN   [2]
RP   REVIEW ON HOST-SELECTIVE TOXINS.
RX   PubMed=22846083; DOI=10.1111/j.1574-6976.2012.00350.x;
RA   Tsuge T., Harimoto Y., Akimitsu K., Ohtani K., Kodama M., Akagi Y.,
RA   Egusa M., Yamamoto M., Otani H.;
RT   "Host-selective toxins produced by the plant pathogenic fungus Alternaria
RT   alternata.";
RL   FEMS Microbiol. Rev. 37:44-66(2013).
CC   -!- FUNCTION: Transcription factor that regulates the expression of the
CC       gene clusters that mediate the biosynthesis of the host-selective
CC       toxins (HSTs) AF-toxins responsible for Alternaria black spot of
CC       strawberry disease by the strawberry pathotype (Probable). On cellular
CC       level, AF-toxins affect plasma membrane of susceptible cells and cause
CC       a sudden increase in loss of K(+) after a few minutes of toxin
CC       treatment (PubMed:22846083). {ECO:0000303|PubMed:22846083,
CC       ECO:0000305|PubMed:12019223}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- MISCELLANEOUS: Gene clusters encoding host-selective toxins (HSTs) are
CC       localized on conditionally dispensable chromosomes (CDCs), also called
CC       supernumerary chromosomes, where they are present in multiple copies
CC       (PubMed:12019223). The CDCs are not essential for saprophytic growth
CC       but controls host-selective pathogenicity (PubMed:12019223).
CC       {ECO:0000269|PubMed:12019223}.
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DR   EMBL; AB070712; BAB69077.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q96VB4; -.
DR   SMR; Q96VB4; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..445
FT                   /note="Transcription activator AFTR-1"
FT                   /id="PRO_0000444838"
FT   DNA_BIND        17..44
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          50..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        68..89
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   445 AA;  48819 MW;  84CC19414E557860 CRC64;
     MMLQCAPKKN ERLRGSCDFC TQSKLRCNKN KPSCRRCTLQ QQPCVYSVAR RTGRPPKHPR
     KANDCQEANG QHGDQDPVTS TPGGSYQQQS NHLLDVEGDG ANFTLADAST TAQGRETAAS
     PALDNALLVG ETFGFSSLLD DPLIQSDDFF SFSLCMPPGE KEGHMASPRT LNGSTGPCSP
     TVLSSIDVPH LPGRFGFLES SVESGLHGRT RPHLVEQPDK TVPSSFSEIE KIYDEGLTFS
     GLDSAINAVT NNGKGEPNIS GTMAAHPHSK RQCFCSTSMS KLQMLVLHPT LCQKNSRARF
     DMALFLEEVV FSIYRDVLQC LVCQSKSLHS LASLCICTDW VIEALRDVAQ DLSSGQDNLG
     GFRAGLYPPK DKFSICVGRF VLDDQLRESC TRSLVRYRLR KLIPIMDTMM KLNHRGAGGA
     LSQAIRTMVE DVHHKIESAL GMMEL