ID   AFTR2_ALTAL             Reviewed;         444 AA.
AC   Q50LF9;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Transcription activator AFTR-2 {ECO:0000303|Ref.1};
DE   AltName: Full=ACT-toxin biosynthesis regulator 2 {ECO:0000303|Ref.1};
GN   Name=AFTR-2 {ECO:0000303|Ref.1};
OS   Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Alternaria; Alternaria alternata complex.
OX   NCBI_TaxID=5599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=NAF8;
RX   DOI=10.1007/s10327-004-0170-3;
RA   Ruswandi S., Kitani K., Akimitsu K., Tsuge T., Shiraishi T., Yamamoto M.;
RT   "Structural analysis of cosmid clone pcAFT-2 carrying AFT10-1 encoding an
RT   acyl-CoA dehydrogenase involved in AF-toxin production in the strawberry
RT   pathotype of Alternaria alternata.";
RL   J. Gen. Plant Pathol. 71:107-116(2005).
RN   [2]
RP   REVIEW ON HOST-SELECTIVE TOXINS.
RX   PubMed=22846083; DOI=10.1111/j.1574-6976.2012.00350.x;
RA   Tsuge T., Harimoto Y., Akimitsu K., Ohtani K., Kodama M., Akagi Y.,
RA   Egusa M., Yamamoto M., Otani H.;
RT   "Host-selective toxins produced by the plant pathogenic fungus Alternaria
RT   alternata.";
RL   FEMS Microbiol. Rev. 37:44-66(2013).
CC   -!- FUNCTION: Transcription factor that regulates the expression of the
CC       gene clusters that mediate the biosynthesis of the host-selective
CC       toxins (HSTs) AF-toxins responsible for Alternaria black spot of
CC       strawberry disease by the strawberry pathotype (Probable). On cellular
CC       level, AF-toxins affect plasma membrane of susceptible cells and cause
CC       a sudden increase in loss of K(+) after a few minutes of toxin
CC       treatment (PubMed:22846083). {ECO:0000303|PubMed:22846083,
CC       ECO:0000305|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- MISCELLANEOUS: Gene clusters encoding host-selective toxins (HSTs) are
CC       localized on conditionally dispensable chromosomes (CDCs), also called
CC       supernumerary chromosomes, where they are present in multiple copies
CC       (Probable). The CDCs are not essential for saprophytic growth but
CC       controls host-selective pathogenicity (Probable). {ECO:0000305|Ref.1}.
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DR   EMBL; AB179766; BAD97698.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50LF9; -.
DR   SMR; Q50LF9; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..444
FT                   /note="Transcription activator AFTR-2"
FT                   /id="PRO_0000444839"
FT   DNA_BIND        16..43
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          49..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        67..88
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   444 AA;  48701 MW;  68931409A4D98239 CRC64;
     MLQCAPKKNE RLRGSCDFCT QSKLRCNKNK PSCRRCTLQQ QPCVYSVARR TGRPPKHPRK
     ANDCQEANGQ HGDQDPVTST PGGSYQQQSN HLLDVEGDGA NFTLADASTT AQGRETAASP
     ALDNALLVGE TFGFSSLLDD PLIQSDDFFS FSLCMPPGEK EGHMASPRTL NGSTGPCSPT
     VLSSIDVPHL PARFGFLESS VESGLHGRTR PHLVEQPDKT VPSSFSEIEK IYDEGLTFSG
     LDSAINAVTN NGKGEPNISG TMAAHPHSKR QCFCSTSMSK LQMLVLHPTL CQKNSRARFD
     MALFLEEVVF SIYRDVLQCL VCQSKSLHSL ASLCICTDWV IEALRDVAQD LSSGQDNLGG
     FRAGLYPPKD KFSICVGRFV LDDQLRESCT RSLVRYRLRK LIPIMDTMMK LNHRGAGGAL
     SQAIRTMVED VHHKIESALG MMEL