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EIF3E_ARATH
ID   EIF3E_ARATH             Reviewed;         441 AA.
AC   Q9C5Z3; Q9M2L8; Q9M4T7;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 151.
DE   RecName: Full=Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004, ECO:0000303|PubMed:11029466};
DE            Short=AtEIF3E-1 {ECO:0000303|PubMed:11029466};
DE            Short=eIF3e {ECO:0000255|HAMAP-Rule:MF_03004, ECO:0000303|PubMed:11029466};
DE            Short=p48 {ECO:0000303|PubMed:11029466};
DE   AltName: Full=Eukaryotic translation initiation factor 3 subunit 6 {ECO:0000255|HAMAP-Rule:MF_03004};
DE            Short=AtINT6 {ECO:0000303|PubMed:11029466};
DE            Short=INT-6 {ECO:0000303|PubMed:11029466};
GN   Name=TIF3E1 {ECO:0000303|PubMed:11029466};
GN   Synonyms=INT6 {ECO:0000303|PubMed:11029466};
GN   OrderedLocusNames=At3g57290 {ECO:0000312|EMBL:AEE79636.1};
GN   ORFNames=F28O9.140 {ECO:0000312|EMBL:CAB68135.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|EMBL:AAG53613.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH CSN7 AND TIF3C1, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=11029466; DOI=10.1074/jbc.m006721200;
RA   Yahalom A., Kim T.-H., Winter E., Karniol B., von Arnim A.G.,
RA   Chamovitz D.A.;
RT   "Arabidopsis eIF3e (INT-6) associates with both eIF3c and the COP9
RT   signalosome subunit CSN7.";
RL   J. Biol. Chem. 276:334-340(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11042177; DOI=10.1074/jbc.m007236200;
RA   Burks E.A., Bezerra P.P., Le H., Gallie D.R., Browning K.S.;
RT   "Plant initiation factor 3 subunit composition resembles mammalian
RT   initiation factor 3 and has a novel subunit.";
RL   J. Biol. Chem. 276:2122-2131(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   INTERACTION WITH TIF3H1.
RX   PubMed=15548739; DOI=10.1105/tpc.104.026880;
RA   Kim T.-H., Kim B.-H., Yahalom A., Chamovitz D.A., von Arnim A.G.;
RT   "Translational regulation via 5' mRNA leader sequences revealed by
RT   mutational analysis of the Arabidopsis translation initiation factor
RT   subunit eIF3h.";
RL   Plant Cell 16:3341-3356(2004).
RN   [7]
RP   PROTEASE-RESISTANCE.
RX   PubMed=16679540; DOI=10.1385/cbb:44:3:522;
RA   Murai M.J., Carneiro F.R.G., Gozzo F.C., Ierardi D.F., Pertinhez T.A.,
RA   Zanchin N.I.T.;
RT   "Identification and characterization of a proteolysis-resistant fragment
RT   containing the PCI domain in the Arabidopsis thaliana INT6/eIF3e
RT   translation factor.";
RL   Cell Biochem. Biophys. 44:522-529(2006).
RN   [8]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=18067529; DOI=10.1111/j.1365-313x.2007.03347.x;
RA   Yahalom A., Kim T.-H., Roy B., Singer R., von Arnim A.G., Chamovitz D.A.;
RT   "Arabidopsis eIF3e is regulated by the COP9 signalosome and has an impact
RT   on development and protein translation.";
RL   Plant J. 53:300-311(2008).
RN   [9]
RP   INTERACTION WITH AT1G27930; AT4G30620; CSN1; CSN4; CSN6A; CSN6B; CSN7;
RP   CSN8; RPN12A; RPS9B AND RPS9C, AND SUBUNIT.
RX   PubMed=19704582; DOI=10.4161/psb.3.6.5434;
RA   Paz-Aviram T., Yahalom A., Chamovitz D.A.;
RT   "Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9
RT   signalosome and proteasome.";
RL   Plant Signal. Behav. 3:409-411(2008).
RN   [10]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH TIF3F1.
RX   PubMed=20444226; DOI=10.1111/j.1365-313x.2010.04237.x;
RA   Xia C., Wang Y.-J., Li W.-Q., Chen Y.-R., Deng Y., Zhang X.-Q., Chen L.-Q.,
RA   Ye D.;
RT   "The Arabidopsis eukaryotic translation initiation factor 3, subunit F
RT   (AteIF3f), is required for pollen germination and embryogenesis.";
RL   Plant J. 63:189-202(2010).
CC   -!- FUNCTION: Component of the eukaryotic translation initiation factor 3
CC       (eIF-3) complex, which is involved in protein synthesis of a
CC       specialized repertoire of mRNAs and, together with other initiation
CC       factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S
CC       ribosome. The eIF-3 complex specifically targets and initiates
CC       translation of a subset of mRNAs involved in cell proliferation
CC       (Potential). Regulates negatively translation during flower development
CC       (PubMed:18067529, PubMed:20444226). {ECO:0000255|HAMAP-Rule:MF_03004,
CC       ECO:0000269|PubMed:18067529, ECO:0000269|PubMed:20444226}.
CC   -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC       (eIF-3) complex (Potential). Binds to the translation initiation
CC       factors TIF3F1 and TIF3H1 (PubMed:20444226, PubMed:15548739).
CC       Associates with the CSN (COP9 signalosome) complex. Interacts directly
CC       with CSN1, CSN4, CSN6A, CSN6B, CSN7, CSN8 and TIF3C1 (PubMed:11029466,
CC       PubMed:19704582). Binds to 40S small ribosomal subunit S9 (RPS9B and
CC       RPS9C) via its N-terminal part. Interacts with the 26S proteasome
CC       subunit RPN12a via its C-terminal part. Binds also with At1g27930 and
CC       At4g30620 (PubMed:19704582). {ECO:0000255|HAMAP-Rule:MF_03004,
CC       ECO:0000269|PubMed:11029466, ECO:0000269|PubMed:15548739,
CC       ECO:0000269|PubMed:19704582, ECO:0000269|PubMed:20444226}.
CC   -!- INTERACTION:
CC       Q9C5Z3; Q9LEZ3: BIM1; NbExp=3; IntAct=EBI-1635572, EBI-617095;
CC       Q9C5Z3; Q94JU3: CSN7; NbExp=6; IntAct=EBI-1635572, EBI-531152;
CC       Q9C5Z3; O49160: TIF3C1; NbExp=3; IntAct=EBI-1635572, EBI-1635551;
CC       Q9C5Z3; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-1635572, EBI-4426557;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03004,
CC       ECO:0000269|PubMed:11029466}. Nucleus {ECO:0000269|PubMed:11029466}.
CC   -!- INDUCTION: Targeted by the COP9 signalosome to proteasome-dependent
CC       degradation. {ECO:0000269|PubMed:18067529}.
CC   -!- DISRUPTION PHENOTYPE: Several floral development defects, including
CC       delayed flowering, reduced sepaloid petals and short stamens. Male
CC       gametophytic lethal. {ECO:0000269|PubMed:18067529,
CC       ECO:0000269|PubMed:20444226}.
CC   -!- MISCELLANEOUS: The PCI domain is protease-resistant.
CC       {ECO:0000269|PubMed:16679540}.
CC   -!- SIMILARITY: Belongs to the eIF-3 subunit E family. {ECO:0000255|HAMAP-
CC       Rule:MF_03004}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB68135.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF255679; AAF67757.1; -; mRNA.
DR   EMBL; AF285832; AAG53613.1; -; mRNA.
DR   EMBL; AL137080; CAB68135.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE79636.1; -; Genomic_DNA.
DR   EMBL; AY039913; AAK64017.1; -; mRNA.
DR   EMBL; AY079357; AAL85088.1; -; mRNA.
DR   PIR; T45807; T45807.
DR   RefSeq; NP_567047.1; NM_115589.5.
DR   AlphaFoldDB; Q9C5Z3; -.
DR   SMR; Q9C5Z3; -.
DR   IntAct; Q9C5Z3; 4.
DR   STRING; 3702.AT3G57290.1; -.
DR   PaxDb; Q9C5Z3; -.
DR   PRIDE; Q9C5Z3; -.
DR   ProteomicsDB; 222285; -.
DR   EnsemblPlants; AT3G57290.1; AT3G57290.1; AT3G57290.
DR   GeneID; 824896; -.
DR   Gramene; AT3G57290.1; AT3G57290.1; AT3G57290.
DR   KEGG; ath:AT3G57290; -.
DR   Araport; AT3G57290; -.
DR   TAIR; locus:2082593; AT3G57290.
DR   eggNOG; KOG2758; Eukaryota.
DR   HOGENOM; CLU_031132_0_0_1; -.
DR   InParanoid; Q9C5Z3; -.
DR   OMA; TWGKLAC; -.
DR   OrthoDB; 1151808at2759; -.
DR   PhylomeDB; Q9C5Z3; -.
DR   PRO; PR:Q9C5Z3; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9C5Z3; baseline and differential.
DR   Genevisible; Q9C5Z3; AT.
DR   GO; GO:0008180; C:COP9 signalosome; IPI:TAIR.
DR   GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0031597; C:cytosolic proteasome complex; IDA:UniProtKB.
DR   GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005852; C:eukaryotic translation initiation factor 3 complex; IPI:TAIR.
DR   GO; GO:0071540; C:eukaryotic translation initiation factor 3 complex, eIF3e; IEA:UniProtKB-UniRule.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0003729; F:mRNA binding; HDA:TAIR.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030371; F:translation repressor activity; IDA:UniProtKB.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; TAS:TAIR.
DR   GO; GO:0009908; P:flower development; IMP:TAIR.
DR   GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0009640; P:photomorphogenesis; IMP:TAIR.
DR   GO; GO:0006412; P:translation; IMP:TAIR.
DR   GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR   HAMAP; MF_03004; eIF3e; 1.
DR   InterPro; IPR016650; eIF3e.
DR   InterPro; IPR019010; eIF3e_N.
DR   InterPro; IPR000717; PCI_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10317; PTHR10317; 1.
DR   Pfam; PF09440; eIF3_N; 1.
DR   Pfam; PF01399; PCI; 1.
DR   PIRSF; PIRSF016255; eIF3e_su6; 1.
DR   SMART; SM01186; eIF3_N; 1.
DR   SMART; SM00088; PINT; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50250; PCI; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Developmental protein; Initiation factor; Nucleus;
KW   Protein biosynthesis; Reference proteome; Repressor;
KW   Translation regulation.
FT   CHAIN           1..441
FT                   /note="Eukaryotic translation initiation factor 3 subunit
FT                   E"
FT                   /id="PRO_0000434569"
FT   DOMAIN          223..407
FT                   /note="PCI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT   CONFLICT        274
FT                   /note="E -> D (in Ref. 1; AAF67757)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        342
FT                   /note="L -> F (in Ref. 1; AAF67757)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        353
FT                   /note="Y -> F (in Ref. 1; AAF67757)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        366
FT                   /note="A -> R (in Ref. 1; AAF67757)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        403
FT                   /note="E -> V (in Ref. 1; AAF67757)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   441 AA;  51750 MW;  A9E6656E51AA1483 CRC64;
     MEESKQNYDL TPLIAPNLDR HLVFPIFEFL QERQLYPDEQ ILKSKIQLLN QTNMVDYAMD
     IHKSLYHTED APQEMVERRT EVVARLKSLE EAAAPLVSFL LNPNAVQELR ADKQYNLQML
     KERYQIGPDQ IEALYQYAKF QFECGNYSGA ADYLYQYRTL CSNLERSLSA LWGKLASEIL
     MQNWDIALEE LNRLKEIIDS KSFSSPLNQV QNRIWLMHWG LYIFFNHDNG RTQIIDLFNQ
     DKYLNAIQTS APHLLRYLAT AFIVNKRRRP QLKEFIKVIQ QEHYSYKDPI IEFLACVFVN
     YDFDGAQKKM KECEEVIVND PFLGKRVEDG NFSTVPLRDE FLENARLFVF ETYCKIHQRI
     DMGVLAEKLN LNYEEAERWI VNLIRTSKLD AKIDSESGTV IMEPTQPNVH EQLINHTKGL
     SGRTYKLVNQ LLEHTQAQAT R
 
 
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