ID   AFUB_ECOLI              Reviewed;         120 AA.
AC   P75681; Q9XB40;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2016, sequence version 3.
DT   25-MAY-2022, entry version 141.
DE   RecName: Full=Putative ferric transport system permease-like protein AfuB;
GN   Name=afuB; Synonyms=fbpB; OrderedLocusNames=b0263, JW0255;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: A severely truncated paralog of the AfuB uptake protein,
CC       homologous only to the last 20% of the intact protein in
CC       Actinobacillus. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein. Note=When
CC       overexpressed using vectors that provide a promoter and ribosome
CC       binding site (PubMed:15919996). {ECO:0000269|PubMed:15919996}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. FbpB subfamily. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. The original protein is
CC       truncated by an IS1 element which is inserted right before residue 6.
CC       What was the original N-terminal of this protein is not known. Other
CC       genes of the operon were presumably also deleted by this IS1 event.
CC       {ECO:0000305}.
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAA77931.2; -; Genomic_DNA.
DR   PIR; G64751; G64751.
DR   AlphaFoldDB; P75681; -.
DR   SMR; P75681; -.
DR   BioGRID; 4263057; 4.
DR   STRING; 316407.85674407; -.
DR   PRIDE; P75681; -.
DR   EnsemblBacteria; BAA77931; BAA77931; BAA77931.
DR   KEGG; ecj:JW0255; -.
DR   PATRIC; fig|83333.103.peg.1017; -.
DR   EchoBASE; EB4025; -.
DR   eggNOG; COG1178; Bacteria.
DR   HOGENOM; CLU_2046117_0_0_6; -.
DR   PhylomeDB; P75681; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   5: Uncertain;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..120
FT                   /note="Putative ferric transport system permease-like
FT                   protein AfuB"
FT                   /id="PRO_0000060016"
FT   TOPO_DOM        1..38
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        60..81
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        103..120
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:15919996"
FT   DOMAIN          1..102
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   120 AA;  13093 MW;  DF63F7D8DB7F8655 CRC64;
     MESLPGQIDK SLDEASLSLR AGSLRTITHI LLPLLRPAIL SALIYSFVRA ITTVSAIVFL
     VTPDTRVATA YILNRVEDGE YGVAIAYGSI LIVVMLAIIF IFDWLIGESR TSRSKAKNQA