ID   AFVD_ASPFN              Reviewed;         259 AA.
AC   B8N8R2;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Methyltransferase afvD {ECO:0000303|PubMed:26209694};
DE            EC=2.1.1.- {ECO:0000305|PubMed:26209694};
DE   AltName: Full=Aflavarin synthesis protein D {ECO:0000303|PubMed:26209694};
GN   Name=afvD {ECO:0000303|PubMed:26209694}; ORFNames=AFLA_108570;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   FUNCTION.
RX   DOI=10.1021/np50086a008;
RA   TePaske M.R., Gloer J.B., Wicklow D.T., Dowd P.F.;
RT   "Aflavarin and beta-aflatrem: new anti-insectan metabolites from the
RT   sclerotia of Aspergillus flavus.";
RL   J. Nat. Prod. 55:1080-1086(1992).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=26209694; DOI=10.1128/ec.00092-15;
RA   Cary J.W., Han Z., Yin Y., Lohmar J.M., Shantappa S., Harris-Coward P.Y.,
RA   Mack B., Ehrlich K.C., Wei Q., Arroyo-Manzanares N., Uka V., Vanhaecke L.,
RA   Bhatnagar D., Yu J., Nierman W.C., Johns M.A., Sorensen D., Shen H.,
RA   De Saeger S., Diana Di Mavungu J., Calvo A.M.;
RT   "Transcriptome analysis of Aspergillus flavus reveals veA-dependent
RT   regulation of secondary metabolite gene clusters, including the novel
RT   aflavarin cluster.";
RL   Eukaryot. Cell 14:983-997(2015).
CC   -!- FUNCTION: Methyltransferase; part of the gene cluster that mediates the
CC       biosynthesis of aflavarin, a bicoumarin that exhibits anti-insectan
CC       activity against the fungivorous beetle C.hemipterus (Ref.2,
CC       PubMed:26209694). {ECO:0000269|PubMed:26209694, ECO:0000269|Ref.2}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:26209694}.
CC   -!- INDUCTION: Expression is induced by the developmental and secondary
CC       metabolism regulator veA (PubMed:26209694).
CC       {ECO:0000269|PubMed:26209694}.
CC   -!- DISRUPTION PHENOTYPE: Fails to produce aflavarin (PubMed:26209694).
CC       {ECO:0000269|PubMed:26209694}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. {ECO:0000305}.
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DR   EMBL; EQ963475; EED53481.1; -; Genomic_DNA.
DR   RefSeq; XP_002376727.1; XM_002376686.1.
DR   AlphaFoldDB; B8N8R2; -.
DR   SMR; B8N8R2; -.
DR   EnsemblFungi; EED53481; EED53481; AFLA_108570.
DR   VEuPathDB; FungiDB:AFLA_108570; -.
DR   eggNOG; ENOG502S4V1; Eukaryota.
DR   HOGENOM; CLU_065416_0_0_1; -.
DR   OMA; HEMLPDW; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR041698; Methyltransf_25.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF13649; Methyltransf_25; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..259
FT                   /note="Methyltransferase afvD"
FT                   /id="PRO_0000436132"
SQ   SEQUENCE   259 AA;  28931 MW;  7D70A9C95A9CB2E2 CRC64;
     MQESLTSGSS ASLNIHDKRF AALYELGGKI TELFAKELIS QSGLPWSSQE PLVVLDNACG
     TGAVSSVLHH TIGNDKKANW HLTCGDKSED MLHYTRQKML QEEWHNAEVK IVNAQDTRLP
     SAHFTHIFTA FAIWVCTLSA AITNLSGDLP APSEKEIHGV YNVGWDEEAS VRAKFEQAGF
     NDIKVRKVIK EYLVPVNQFV ESCTILIPTI VNIFWTQDQR DQYESELPMA VHRYVEGKYG
     RDGMASMEAE AIIATGHKH