EIF3I_ARATH
ID EIF3I_ARATH Reviewed; 328 AA.
AC Q38884; O82342; Q94K09; Q9C5Z0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2002, sequence version 2.
DT 25-MAY-2022, entry version 150.
DE RecName: Full=Eukaryotic translation initiation factor 3 subunit I {ECO:0000255|HAMAP-Rule:MF_03008};
DE Short=eIF3i {ECO:0000255|HAMAP-Rule:MF_03008};
DE AltName: Full=Eukaryotic translation initiation factor 3 subunit 2 {ECO:0000255|HAMAP-Rule:MF_03008};
DE AltName: Full=TGF-beta receptor-interacting protein 1;
DE Short=TRIP-1;
DE AltName: Full=eIF-3-beta {ECO:0000255|HAMAP-Rule:MF_03008};
DE AltName: Full=eIF3 p36 {ECO:0000255|HAMAP-Rule:MF_03008};
GN Name=TIF3I1; OrderedLocusNames=At2g46280; ORFNames=T3F17.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7566156; DOI=10.1038/377548a0;
RA Chen R.H., Miettinen P.J., Maruka E.M., Choy L., Derynck R.;
RT "A WD-domain protein that is associated with and phosphorylated by the type
RT II TGF-beta receptor.";
RL Nature 377:548-552(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11042177; DOI=10.1074/jbc.m007236200;
RA Burks E.A., Bezerra P.P., Le H., Gallie D.R., Browning K.S.;
RT "Plant initiation factor 3 subunit composition resembles mammalian
RT initiation factor 3 and has a novel subunit.";
RL J. Biol. Chem. 276:2122-2131(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex, which is involved in protein synthesis of a
CC specialized repertoire of mRNAs and, together with other initiation
CC factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S
CC ribosome. The eIF-3 complex specifically targets and initiates
CC translation of a subset of mRNAs involved in cell proliferation.
CC {ECO:0000255|HAMAP-Rule:MF_03008}.
CC -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex. {ECO:0000255|HAMAP-Rule:MF_03008}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03008}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q38884-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the eIF-3 subunit I family. {ECO:0000255|HAMAP-
CC Rule:MF_03008}.
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DR EMBL; U36765; AAC49079.1; -; mRNA.
DR EMBL; AF285835; AAG53616.1; -; mRNA.
DR EMBL; AC005397; AAC62878.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10670.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10671.1; -; Genomic_DNA.
DR EMBL; AF370485; AAK43862.1; -; mRNA.
DR EMBL; AY064633; AAL47346.1; -; mRNA.
DR PIR; H84900; H84900.
DR PIR; S60256; S60256.
DR RefSeq; NP_182151.1; NM_130191.6. [Q38884-1]
DR RefSeq; NP_850450.1; NM_180119.3. [Q38884-1]
DR AlphaFoldDB; Q38884; -.
DR SMR; Q38884; -.
DR BioGRID; 4571; 27.
DR IntAct; Q38884; 2.
DR STRING; 3702.AT2G46280.1; -.
DR PaxDb; Q38884; -.
DR PRIDE; Q38884; -.
DR ProteomicsDB; 221938; -. [Q38884-1]
DR EnsemblPlants; AT2G46280.1; AT2G46280.1; AT2G46280. [Q38884-1]
DR EnsemblPlants; AT2G46280.2; AT2G46280.2; AT2G46280. [Q38884-1]
DR GeneID; 819236; -.
DR Gramene; AT2G46280.1; AT2G46280.1; AT2G46280. [Q38884-1]
DR Gramene; AT2G46280.2; AT2G46280.2; AT2G46280. [Q38884-1]
DR KEGG; ath:AT2G46280; -.
DR Araport; AT2G46280; -.
DR TAIR; locus:2063009; AT2G46280.
DR eggNOG; KOG0643; Eukaryota.
DR HOGENOM; CLU_043845_0_1_1; -.
DR InParanoid; Q38884; -.
DR OMA; VWFSHNG; -.
DR PhylomeDB; Q38884; -.
DR PRO; PR:Q38884; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q38884; baseline and differential.
DR Genevisible; Q38884; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IPI:TAIR.
DR GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0071541; C:eukaryotic translation initiation factor 3 complex, eIF3m; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03008; eIF3i; 1.
DR InterPro; IPR027525; eIF3i.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Initiation factor; Protein biosynthesis;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..328
FT /note="Eukaryotic translation initiation factor 3 subunit
FT I"
FT /id="PRO_0000051039"
FT REPEAT 8..49
FT /note="WD 1"
FT REPEAT 50..89
FT /note="WD 2"
FT REPEAT 146..185
FT /note="WD 3"
FT REPEAT 191..230
FT /note="WD 4"
FT REPEAT 288..327
FT /note="WD 5"
FT CONFLICT 21..23
FT /note="EGD -> QPH (in Ref. 2; AAG53616)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="L -> F (in Ref. 2; AAG53616)"
FT /evidence="ECO:0000305"
FT CONFLICT 126..128
FT /note="RIA -> AYC (in Ref. 1; AAC49079)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="G -> C (in Ref. 1; AAC49079)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="Q -> E (in Ref. 1; AAC49079)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="Y -> C (in Ref. 1; AAC49079)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="S -> G (in Ref. 5; AAK43862/AAL47346)"
FT /evidence="ECO:0000305"
FT CONFLICT 311
FT /note="D -> G (in Ref. 1; AAC49079)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 36388 MW; 4ABFE9866A09CFAF CRC64;
MRPILMKGHE RPLTFLRYNR EGDLLFSCAK DHTPTLWFAD NGERLGTYRG HNGAVWCCDV
SRDSSRLITG SADQTAKLWD VKSGKELFTF KFNAPTRSVD FAVGDRLAVI TTDHFVDRTA
AIHVKRIAED PEEQDAESVL VLHCPDGKKR INRAVWGPLN QTIVSGGEDK VIRIWDAETG
KLLKQSDEEV GHKKDITSLC KAADDSHFLT GSLDKTAKLW DMRTLTLLKT YTTVVPVNAV
SLSPLLNHVV LGGGQDASAV TTTDHRAGKF EAKFYDKILQ EEIGGVKGHF GPINALAFNP
DGKSFSSGGE DGYVRLHHFD SDYFNIKI
