ID   AG2_XENLA               Reviewed;         185 AA.
AC   P55869; O42251;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 3.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Anterior gradient protein 2;
DE            Short=XAG-2;
DE   AltName: Full=Secreted protein np77;
DE   Flags: Precursor;
GN   Name=ag2; Synonyms=np77;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Embryonic head;
RX   PubMed=2752418; DOI=10.1016/0092-8674(89)90413-3;
RA   Sive H.L., Hattori K., Weintraub H.;
RT   "Progressive determination during formation of the anteroposterior axis in
RT   Xenopus laevis.";
RL   Cell 58:171-180(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Neurula;
RX   PubMed=9533957; DOI=10.1016/s0925-4773(98)00021-5;
RA   Aberger F., Weidinger G., Grunz H., Richter K.;
RT   "Anterior specification of embryonic ectoderm: the role of the Xenopus
RT   cement gland-specific gene XAG-2.";
RL   Mech. Dev. 72:115-130(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tail bud;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in cement gland formation; probably specifies dorsal
CC       ectoderm to acquire an anterior fate such as cement gland and
CC       forebrain. Signals via the FGF pathway. {ECO:0000269|PubMed:9533957}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9533957}.
CC   -!- TISSUE SPECIFICITY: Expressed in the anterior of the dorsal ectoderm
CC       from late gastrula stages onwards. Becomes restricted to the cement
CC       gland anlage at the onset of neurulation (stages 13 to 14) and
CC       expressed exclusively in the cement gland from stage 18 onwards, with
CC       transient expression in the hatching gland during tailbud stages.
CC       {ECO:0000269|PubMed:2752418, ECO:0000269|PubMed:9533957}.
CC   -!- INDUCTION: In response to organizer-secreted signals and neural
CC       inducers.
CC   -!- SIMILARITY: Belongs to the AGR family. {ECO:0000305}.
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DR   EMBL; AF025474; AAB81968.1; -; mRNA.
DR   EMBL; U82110; AAB49974.1; -; mRNA.
DR   EMBL; BC078083; AAH78083.1; -; mRNA.
DR   RefSeq; NP_001081669.1; NM_001088200.1.
DR   AlphaFoldDB; P55869; -.
DR   SMR; P55869; -.
DR   DNASU; 397989; -.
DR   GeneID; 397989; -.
DR   KEGG; xla:397989; -.
DR   CTD; 397989; -.
DR   Xenbase; XB-GENE-6252140; ag1.S.
DR   OMA; EDIEWAQ; -.
DR   OrthoDB; 1382017at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10S.
DR   Bgee; 397989; Expressed in neurula embryo and 9 other tissues.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0071570; P:cement gland development; IMP:UniProtKB.
DR   GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IMP:UniProtKB.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   SUPFAM; SSF52833; SSF52833; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..185
FT                   /note="Anterior gradient protein 2"
FT                   /id="PRO_0000001043"
FT   REGION          25..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        30
FT                   /note="G -> A (in Ref. 1; AAB49974)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        137..138
FT                   /note="RV -> KG (in Ref. 1; AAB49974)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   185 AA;  20499 MW;  D6CDE02DEC3B857B CRC64;
     MQTGLSLACL VLLCSVLGEA ALRKPKRQAG ATDTNGAAKS EPAPVKTKGL KTLDRGWGED
     IEWAQTYEEG LAKARENNKP LMVIHHLEDC PYSIALKKAF VADKMAQKLA QEDFIMLNLV
     HPVADENQSP DGHYVPRVIF IDPSLTVRSD LKGRYGNKLY AYDADDIPEL ITNMKKAKSF
     LKTEL