AG5L1_CULNU
ID AG5L1_CULNU Reviewed; 219 AA.
AC B9URJ1;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 35.
DE RecName: Full=Antigen 5 like allergen Cul n 1 {ECO:0000303|PubMed:20537727};
DE Short=Ag5 like allergen Cul n 1 {ECO:0000303|PubMed:20537727};
DE AltName: Full=Allergen Culn1 {ECO:0000303|PubMed:23891138};
DE AltName: Full=Antigen 5 family protein {ECO:0000303|PubMed:19523070};
DE AltName: Full=Cul n 12 {ECO:0000305};
DE Flags: Precursor;
OS Culicoides nubeculosus (Biting midge).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Chironomoidea;
OC Ceratopogonidae; Ceratopogoninae; Culicoides; Monoculicoides.
OX NCBI_TaxID=144565 {ECO:0000312|EMBL:ACM40888.1};
RN [1] {ECO:0000312|EMBL:ACM40888.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Salivary gland {ECO:0000303|PubMed:20537727,
RC ECO:0000312|EMBL:ACM40888.1};
RX PubMed=19523070; DOI=10.1111/j.1365-2583.2009.00882.x;
RA Russell C.L., Heesom K.J., Arthur C.J., Helps C.R., Mellor P.S., Day M.J.,
RA Torsteinsdottir S., Bjoernsdottir T.S., Wilson A.D.;
RT "Identification and isolation of cDNA clones encoding the abundant secreted
RT proteins in the saliva proteome of Culicoides nubeculosus.";
RL Insect Mol. Biol. 18:383-393(2009).
RN [2]
RP TISSUE SPECIFICITY, AND ALLERGEN.
RX PubMed=20537727; DOI=10.1016/j.vetimm.2010.04.012;
RA Schaffartzik A., Marti E., Crameri R., Rhyner C.;
RT "Cloning, production and characterization of antigen 5 like proteins from
RT Simulium vittatum and Culicoides nubeculosus, the first cross-reactive
RT allergen associated with equine insect bite hypersensitivity.";
RL Vet. Immunol. Immunopathol. 137:76-83(2010).
RN [3]
RP ALLERGEN.
RX PubMed=23891138; DOI=10.1016/j.tvjl.2013.06.010;
RA Peeters L.M., Janssens S., Goddeeris B.M., De Keyser K., Wilson A.D.,
RA Kaufmann C., Schaffartzik A., Marti E., Buys N.;
RT "Evaluation of an IgE ELISA with Culicoides spp. extracts and recombinant
RT salivary antigens for diagnosis of insect bite hypersensitivity in
RT Warmblood horses.";
RL Vet. J. 198:141-147(2013).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19523070,
CC ECO:0000305|PubMed:20537727}.
CC -!- TISSUE SPECIFICITY: Expressed in salivary glands.
CC {ECO:0000269|PubMed:19523070, ECO:0000269|PubMed:20537727}.
CC -!- ALLERGEN: Causes an allergic reaction in horses (PubMed:20537727,
CC PubMed:23891138). Binds to IgE in 35% of the 48 Icelandic horses living
CC in Switzerland tested affected by insect bite hypersensitivity (IBH), a
CC recurrent seasonal dermatitis of the horse, caused by bites of
CC Culicoides (midge) and sometimes Simulium (black fly) species. Does not
CC bind to IgE of 27 horses out of the 29 horses living in Switzerland (24
CC Icelandic, 2 Swiss Warmbloods and 2 Shetland ponies) tested unaffected
CC by IBH. Clear immediate and late phase hypersensitivity reactions to
CC this protein in IBH-affected horses are evident by intradermal skin
CC test (IDT), whereas control horses unaffected by IBH do not develop
CC relevant immediate hypersensitivity reactions (PubMed:20537727). Binds
CC to IgE of 18% of the 167 horses living in Belgium tested affected by
CC IBH. Binds to IgE of 6% of the 176 horses living in Belgium tested not
CC affected by IBH (PubMed:23891138). {ECO:0000269|PubMed:20537727,
CC ECO:0000269|PubMed:23891138}.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; EU978899; ACM40888.1; -; mRNA.
DR AlphaFoldDB; B9URJ1; -.
DR SMR; B9URJ1; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR034763; P14a_insect.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PIRSF; PIRSF038921; P14a; 1.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..219
FT /note="Antigen 5 like allergen Cul n 1"
FT /evidence="ECO:0000255"
FT /id="PRO_5002891115"
FT DOMAIN 73..211
FT /note="SCP"
FT /evidence="ECO:0000255"
FT DISULFID 23..45
FT /evidence="ECO:0000250|UniProtKB:P35778"
FT DISULFID 28..124
FT /evidence="ECO:0000250|UniProtKB:P35778"
FT DISULFID 55..117
FT /evidence="ECO:0000250|UniProtKB:P35778"
SQ SEQUENCE 219 AA; 25297 MW; A523F0C9E8BA4908 CRC64;
MIKKLSIVIL FSCISFVLST NFCNKELCKR QNGPQSFTYL KHIGCRHTGK NANTCPRDAK
ILPMSTKRKN LILKVHNRLR NKVALGKLPG FPKAARMPIL RWDDELAYLA ELNVKQCKME
HDQCRNTDKF KYAGQNLAYT MGTPQKNAVR IKKLIRAWFK EHENATASFI DKYRDHPQGR
VIGHFTAMIQ DRTDTVGCAI LRHSGNKYFF WPVIMVLQI
