EIF3K_BOVIN
ID EIF3K_BOVIN Reviewed; 218 AA.
AC Q3T0V3;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Eukaryotic translation initiation factor 3 subunit K {ECO:0000255|HAMAP-Rule:MF_03010};
DE Short=eIF3k {ECO:0000255|HAMAP-Rule:MF_03010};
DE AltName: Full=Eukaryotic translation initiation factor 3 subunit 12 {ECO:0000255|HAMAP-Rule:MF_03010};
DE AltName: Full=eIF-3 p25 {ECO:0000255|HAMAP-Rule:MF_03010};
GN Name=EIF3K {ECO:0000255|HAMAP-Rule:MF_03010};
GN Synonyms=EIF3S12 {ECO:0000255|HAMAP-Rule:MF_03010};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex, which is required for several steps in the initiation
CC of protein synthesis. The eIF-3 complex associates with the 40S
CC ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-
CC 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex
CC (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC
CC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also
CC required for disassembly and recycling of post-termination ribosomal
CC complexes and subsequently prevents premature joining of the 40S and
CC 60S ribosomal subunits prior to initiation. The eIF-3 complex
CC specifically targets and initiates translation of a subset of mRNAs
CC involved in cell proliferation, including cell cycling, differentiation
CC and apoptosis, and uses different modes of RNA stem-loop binding to
CC exert either translational activation or repression.
CC {ECO:0000255|HAMAP-Rule:MF_03010}.
CC -!- SUBUNIT: Component of the eukaryotic translation initiation factor 3
CC (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C,
CC EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and
CC EIF3M. The eIF-3 complex appears to include 3 stable modules: module A
CC is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of
CC EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D,
CC EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and
CC EIF3H of module B, thereby linking the three modules. EIF3J is a labile
CC subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex
CC interacts with RPS6KB1 under conditions of nutrient depletion.
CC Mitogenic stimulation leads to binding and activation of a complex
CC composed of MTOR and RPTOR, leading to phosphorylation and release of
CC RPS6KB1 and binding of EIF4B to eIF-3. Interacts with CCND3, but not
CC with CCND1 and CCND2. {ECO:0000255|HAMAP-Rule:MF_03010}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03010}.
CC Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03010}.
CC -!- SIMILARITY: Belongs to the eIF-3 subunit K family. {ECO:0000255|HAMAP-
CC Rule:MF_03010}.
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DR EMBL; BC102250; AAI02251.1; -; mRNA.
DR RefSeq; NP_001029661.1; NM_001034489.2.
DR AlphaFoldDB; Q3T0V3; -.
DR SMR; Q3T0V3; -.
DR STRING; 9913.ENSBTAP00000014888; -.
DR PaxDb; Q3T0V3; -.
DR PRIDE; Q3T0V3; -.
DR Ensembl; ENSBTAT00000014888; ENSBTAP00000014888; ENSBTAG00000011212.
DR GeneID; 515326; -.
DR KEGG; bta:515326; -.
DR CTD; 27335; -.
DR VEuPathDB; HostDB:ENSBTAG00000011212; -.
DR VGNC; VGNC:28401; EIF3K.
DR eggNOG; KOG3252; Eukaryota.
DR GeneTree; ENSGT00390000009409; -.
DR HOGENOM; CLU_076723_1_0_1; -.
DR InParanoid; Q3T0V3; -.
DR OMA; WKHQGQG; -.
DR OrthoDB; 1576799at2759; -.
DR TreeFam; TF314893; -.
DR Reactome; R-BTA-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Proteomes; UP000009136; Chromosome 18.
DR Bgee; ENSBTAG00000011212; Expressed in digestive system secreted substance and 105 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:UniProtKB-UniRule.
DR GO; GO:0006446; P:regulation of translational initiation; IEA:InterPro.
DR GO; GO:0006413; P:translational initiation; ISS:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.25.40.250; -; 1.
DR HAMAP; MF_03010; eIF3k; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033464; CSN8_PSD8_EIF3K.
DR InterPro; IPR009374; eIF3k.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR016020; Transl_init_fac_sub12_N_euk.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13022; PTHR13022; 1.
DR Pfam; PF10075; CSN8_PSD8_EIF3K; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50250; PCI; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Initiation factor; Nucleus; Phosphoprotein;
KW Protein biosynthesis; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03010"
FT CHAIN 2..218
FT /note="Eukaryotic translation initiation factor 3 subunit
FT K"
FT /id="PRO_0000123545"
FT DOMAIN 42..204
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9UBQ5, ECO:0000255|HAMAP-
FT Rule:MF_03010"
FT MOD_RES 28
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9UBQ5"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UBQ5"
SQ SEQUENCE 218 AA; 25074 MW; 1C2CBBE8AC81F28A CRC64;
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF
FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA
LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE MLGDLTDSQL KVWMSKYGWS
ADESGQIFIC SQEESIKPKN IVEKIDFDSV SSIMASSQ
