ID   AGAB_VIBS7              Reviewed;         955 AA.
AC   P48840;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Beta-agarase B;
DE            EC=3.2.1.81;
GN   Name=agaB;
OS   Vibrio sp. (strain JT0107).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=47913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8193156; DOI=10.1016/0167-4781(94)90109-0;
RA   Sugano Y., Matsumoto T., Noma M.;
RT   "Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio
RT   sp. strain JT0107.";
RL   Biochim. Biophys. Acta 1218:105-108(1994).
CC   -!- FUNCTION: Hydrolyzes agarose to yield predominantly neoagarotetraose
CC       and neoagarohexaose.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose,
CC         giving the tetramer as the predominant product.; EC=3.2.1.81;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 50 family. {ECO:0000305}.
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DR   EMBL; D21202; BAA04744.1; -; Genomic_DNA.
DR   PIR; S46651; S46651.
DR   AlphaFoldDB; P48840; -.
DR   SMR; P48840; -.
DR   CAZy; GH50; Glycoside Hydrolase Family 50.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0033916; F:beta-agarase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR040669; Agarase_CBM.
DR   InterPro; IPR013529; Glyco_hydro_42_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF17992; Agarase_CBM; 2.
DR   Pfam; PF02449; Glyco_hydro_42; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..955
FT                   /note="Beta-agarase B"
FT                   /id="PRO_0000057698"
SQ   SEQUENCE   955 AA;  105977 MW;  6E54555DDA8BD215 CRC64;
     MVEVMKFTKN KIAALLSLTL LGVYGCGSTP SSSDAEGAVE DVGGTIPDFE SAAFFKKVKK
     DHRKAEVVSD QGVTSGSSAL KVNFDSVSEA NKFKYWPNVK VHPDSGFWNW NAKGSLSLDI
     TNPTDSPANI ILKLADNVGV MGSGDNQLNY AVNVPAGETV PVEMLFNGTK RKLDGYWGGE
     KINLRNIVEF QIFVQGPMDA QTVIIDNFNL VDATGDFIEA SGQEVKVSGP IPTVASITSF
     DEGQPTFVAF DRSAAATVTE LKTDMGGLLA VKLAATNAYP NITFKAPQPW DWSEYGDFSL
     AFDLESKADE PLQLFVRVDD AENENWGGTA NGVVDSMSSY VTLAPGDDGT FYLPLGQTGS
     QIVSGMRAEP PKKSYNAQAI SYGWGEKSLD TSNIVSFQLY LQNPTKDAEF NIKSVRLIPN
     IDADATRYEG LIDQYGQFTG SEWPKKITED EELETMGKLA KMSLKSTSQM PGRSIYGGWA
     DGPKLKGTGF FRTEKVDGKW SLVDPQGNLF FATGVDNIRM DDTVTITGHD FADKDKRSGK
     EVASEVRRSM FTWLPEDDDV LAENYDYANW VHSGALKKGE VFSFYGANLQ RKYGGTFSEA
     EKVWKDITID RMVDWGFTTL GNWADPMFYD NKKVAYVANG WIFGDHARIS TGNDYWGPIH
     DPFDPEFVNS VKAMTKKLMT EVDKNDPWMM GVFVDNEISW GNTKNDANHY GLVVNALSYD
     MKKSPAKAAF TEHLKEKYWA IEDLNTSWGV KVASWAEFEK SFDHRSRLSK NMKKDYAEML
     EMLSAKYFST VRAELKKVLP NHLYLGAPFA DWGVTPEIAK GAAPYVDVMS YNLYAEDLNS
     KGDWSKLAEL DKPSIIGEFH FGSTDSGLFH GGIVSAASQQ DRAKKYTNYM NSIADNPYFV
     GAHWFQYIDS PTTGRAWDGE NYNVGFVSIT DTPYVPLVEA AKKFNQDVYM LRYKK