EIL1A_ORYSJ
ID EIL1A_ORYSJ Reviewed; 641 AA.
AC Q10M41; Q0DS96; Q3Y6P4;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Protein ETHYLENE-INSENSITIVE 3-like 1a {ECO:0000303|PubMed:16786297};
DE Short=EIN3-like protein 1a {ECO:0000303|PubMed:16786297};
DE Short=OsEIL1a {ECO:0000303|PubMed:16786297};
DE AltName: Full=OsEIL1 {ECO:0000303|PubMed:16786297};
DE AltName: Full=Protein MAO HUZI 6 {ECO:0000303|PubMed:25995326};
GN Name=EIL1A {ECO:0000303|PubMed:16786297};
GN Synonyms=EIL1 {ECO:0000303|PubMed:16786297},
GN MHZ6 {ECO:0000303|PubMed:25995326};
GN OrderedLocusNames=Os03g0324300 {ECO:0000312|EMBL:BAS83971.1},
GN LOC_Os03g20790 {ECO:0000312|EMBL:ABF95697.1};
GN ORFNames=OsJ_10672 {ECO:0000312|EMBL:EAZ26760.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2-641, AND FUNCTION.
RX PubMed=16786297; DOI=10.1007/s11103-005-6184-1;
RA Mao C., Wang S., Jia Q., Wu P.;
RT "OsEIL1, a rice homolog of the Arabidopsis EIN3 regulates the ethylene
RT response as a positive component.";
RL Plant Mol. Biol. 61:141-152(2006).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=25995326; DOI=10.1104/pp.15.00353;
RA Yang C., Ma B., He S.J., Xiong Q., Duan K.X., Yin C.C., Chen H., Lu X.,
RA Chen S.Y., Zhang J.S.;
RT "MAOHUZI6/ETHYLENE INSENSITIVE3-LIKE1 and ETHYLENE INSENSITIVE3-LIKE2
RT regulate ethylene response of roots and coleoptiles and negatively affect
RT salt tolerance in rice.";
RL Plant Physiol. 169:148-165(2015).
RN [9]
RP FUNCTION, AND INDUCTION BY RICE BLAST FUNGUS.
RX PubMed=27701783; DOI=10.1111/tpj.13388;
RA Yang C., Li W., Cao J., Meng F., Yu Y., Huang J., Jiang L., Liu M.,
RA Zhang Z., Chen X., Miyamoto K., Yamane H., Zhang J., Chen S., Liu J.;
RT "Activation of ethylene signaling pathways enhances disease resistance by
RT regulating ROS and phytoalexin production in rice.";
RL Plant J. 89:338-353(2017).
RN [10]
RP FUNCTION.
RC STRAIN=cv. Hwayoung;
RX PubMed=28829777; DOI=10.1371/journal.pgen.1006955;
RA Qin H., Zhang Z., Wang J., Chen X., Wei P., Huang R.;
RT "The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is
RT required for ethylene-inhibited root elongation in rice early seedling
RT development.";
RL PLoS Genet. 13:E1006955-E1006955(2017).
RN [11]
RP FUNCTION, AND MUTAGENESIS OF LYS-282.
RX PubMed=30002253; DOI=10.1126/science.aat1577;
RA Kuroha T., Nagai K., Gamuyao R., Wang D.R., Furuta T., Nakamori M.,
RA Kitaoka T., Adachi K., Minami A., Mori Y., Mashiguchi K., Seto Y.,
RA Yamaguchi S., Kojima M., Sakakibara H., Wu J., Ebana K., Mitsuda N.,
RA Ohme-Takagi M., Yanagisawa S., Yamasaki M., Yokoyama R., Nishitani K.,
RA Mochizuki T., Tamiya G., McCouch S.R., Ashikari M.;
RT "Ethylene-gibberellin signaling underlies adaptation of rice to periodic
RT flooding.";
RL Science 361:181-186(2018).
CC -!- FUNCTION: Transcription factor acting as a positive regulator in the
CC ethylene response pathway (PubMed:16786297, PubMed:28829777,
CC PubMed:27701783, PubMed:25995326). Required for the inhibition of root
CC growth by ethylene in etiolated seedlings (PubMed:25995326,
CC PubMed:28829777). Functions upstream of the auxin biosynthetic gene
CC YUCCA8 and directly activates its expression (PubMed:28829777).
CC Functions downstream of the ethylene signaling factor EIN2 in disease
CC resistance against the rice blast fungus (Magnaporthe oryzae)
CC (PubMed:27701783). Binds directly to the promoters of the NADPH
CC oxidases RBOHA and RBOHB, and the jasmonate biosynthetic gene OPR4 to
CC activate their expression during fungal infection (PubMed:27701783).
CC May enhance disease resistance by facilitating reactive oxygen species
CC (ROS) generation and jasmonate biosynthesis with subsequent phytoalexin
CC accumulation during Magnaporthe oryzae infection (PubMed:27701783).
CC Acts as negative regulator of salt tolerance (PubMed:25995326). During
CC salt stress, activates the cation transporter HKT1, which mediates
CC increased sodium uptake in roots, and contributes to sodium
CC accumulation and salt toxicity (PubMed:25995326). Binds directly to the
CC DNA sequence 5'-TGTTACAAATACC-3' in the promoter of the GA20OX2 gene to
CC activate its expression at the transcriptional level during ethylene
CC signaling (PubMed:30002253). Possesses transactivation activity in
CC protoplasts (PubMed:25995326). {ECO:0000269|PubMed:16786297,
CC ECO:0000269|PubMed:25995326, ECO:0000269|PubMed:27701783,
CC ECO:0000269|PubMed:28829777, ECO:0000269|PubMed:30002253}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25995326}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf blades, leaf sheaths,
CC stems, internodes, anthers of young flowers, and developing grains.
CC {ECO:0000269|PubMed:25995326}.
CC -!- INDUCTION: Induced by infection with the fungal pathogen Magnaporthe
CC oryzae. {ECO:0000269|PubMed:27701783}.
CC -!- DISRUPTION PHENOTYPE: Insensitive to ethylene-induced root growth
CC inhibition. {ECO:0000269|PubMed:25995326}.
CC -!- MISCELLANEOUS: Plants overexpressing EIL1 exhibit short root, coiled
CC primary root, short shoot phenotype, and elevated response to exogenous
CC ethylene (PubMed:16786297, PubMed:25995326). Plants overexpressing EIL1
CC exhibit resistance to the fungal pathogen Magnaporthe oryzae
CC (PubMed:27701783). 'Mao huzi' means cat whiskers in Chinese (Probable).
CC {ECO:0000269|PubMed:16786297, ECO:0000269|PubMed:25995326,
CC ECO:0000269|PubMed:27701783, ECO:0000305|PubMed:25995326}.
CC -!- SIMILARITY: Belongs to the EIN3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF11892.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAS83972.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ26760.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DP000009; ABF95697.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11892.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS83971.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83972.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000140; EAZ26760.1; ALT_INIT; Genomic_DNA.
DR EMBL; AK103227; BAG95963.1; -; mRNA.
DR EMBL; DQ153245; AAZ78349.1; -; mRNA.
DR RefSeq; XP_015629858.1; XM_015774372.1.
DR AlphaFoldDB; Q10M41; -.
DR SMR; Q10M41; -.
DR STRING; 4530.OS03T0324300-02; -.
DR PaxDb; Q10M41; -.
DR EnsemblPlants; Os03t0324300-02; Os03t0324300-02; Os03g0324300.
DR EnsemblPlants; Os03t0324300-04; Os03t0324300-04; Os03g0324300.
DR EnsemblPlants; Os03t0324300-05; Os03t0324300-05; Os03g0324300.
DR GeneID; 107276358; -.
DR Gramene; Os03t0324300-02; Os03t0324300-02; Os03g0324300.
DR Gramene; Os03t0324300-04; Os03t0324300-04; Os03g0324300.
DR Gramene; Os03t0324300-05; Os03t0324300-05; Os03g0324300.
DR KEGG; osa:107276358; -.
DR eggNOG; ENOG502QQSG; Eukaryota.
DR OMA; DNHQFAC; -.
DR OrthoDB; 266873at2759; -.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q10M41; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0009871; P:jasmonic acid and ethylene-dependent systemic resistance, ethylene mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:1901001; P:negative regulation of response to salt stress; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:UniProtKB.
DR Gene3D; 1.10.3180.10; -; 2.
DR InterPro; IPR006957; EIN3.
DR InterPro; IPR023278; Ethylene_insens-like_DNA-bd.
DR PANTHER; PTHR33305; PTHR33305; 1.
DR Pfam; PF04873; EIN3; 1.
DR SUPFAM; SSF116768; SSF116768; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Stress response.
FT CHAIN 1..641
FT /note="Protein ETHYLENE-INSENSITIVE 3-like 1a"
FT /id="PRO_0000445282"
FT REGION 47..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 97..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 282
FT /note="K->N: Abolishes transactivation activity towards
FT GA20OX2."
FT /evidence="ECO:0000269|PubMed:30002253"
SQ SEQUENCE 641 AA; 70945 MW; B63F8C78BBBA4323 CRC64;
MMGGGLVMDQ GMMFPGVHNF VDLLQQNGGD KNLGFGALVP QTSSGEQCVM GEGDLVDPPP
ESFPDAGEDD SDDDVEDIEE LERRMWRDRM KLKRLKELQL SRGKDPAGGV VGDPSKPRQS
QEQARRKKMS RAQDGILKYM LKMMEVCRAQ GFVYGIIPEK GKPVSGASDN LRGWWKEKVR
FDRNGPAAIA KYQADNAVPG FESELASGTG SPHSLQELQD TTLGSLLSAL MQHCDPPQRR
YPLEKGVPPP WWPTGDEEWW PELGIPKDQG PPPYKKPHDL KKAWKVSVLT AVIKHMSPDI
EKIRRLVRQS KCLQDKMTAK EISTWLAVVK QEEELYLKLN PGARPPAPTG GITSAISFNA
SSSEYDVDVV DDCKGDEAGN QKAVVVADPT AFNLGAAMLN DKFLMPASMK EEATDVEFIQ
KRSASGAEPE LMLNNRVYTC HNVQCPHSDY GYGFLDRNAR NSHQYTCKYN DPLQQSTENK
PSPPAIFPAT YNTPNQALNN LDFGLPMDGQ RSITELMNMY DNNFVANKNL SNDNATIMER
PNAVNPRIQI EEGFFGQGSG IGGSNGGVFE DVNGMMQQPQ QTTPAQQQFF IRDDTPFGNQ
MGDINGASEF RFGSGFNMSG AVEYPGAMQG QQKNDGSNWY Y
