EIL1_ARATH
ID EIL1_ARATH Reviewed; 584 AA.
AC Q9SLH0; O23114;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=ETHYLENE INSENSITIVE 3-like 1 protein;
GN Name=EIL1; OrderedLocusNames=At2g27050; ORFNames=T20P8.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=9215635; DOI=10.1016/s0092-8674(00)80300-1;
RA Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R.;
RT "Activation of the ethylene gas response pathway in Arabidopsis by the
RT nuclear protein ETHYLENE-INSENSITIVE3 and related proteins.";
RL Cell 89:1133-1144(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP CHARACTERIZATION, AND FUNCTION.
RX PubMed=9851977; DOI=10.1101/gad.12.23.3703;
RA Solano R., Stepanova A.N., Chao Q., Ecker J.R.;
RT "Nuclear events in ethylene signaling: a transcriptional cascade mediated
RT by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.";
RL Genes Dev. 12:3703-3714(1998).
RN [6]
RP CHARACTERIZATION, AND MUTANTS EIL1-1 AND EIL1-2.
RX PubMed=12606727; DOI=10.1073/pnas.0438070100;
RA Alonso J.M., Stepanova A.N., Solano R., Wisman E., Ferrari S.,
RA Ausubel F.M., Ecker J.R.;
RT "Five components of the ethylene-response pathway identified in a screen
RT for weak ethylene-insensitive mutants in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2992-2997(2003).
CC -!- FUNCTION: Probable transcription factor acting as a positive regulator
CC in the ethylene response pathway. Could bind the primary ethylene
CC response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter.
CC {ECO:0000269|PubMed:9215635, ECO:0000269|PubMed:9851977}.
CC -!- SUBUNIT: Acts as homodimer to bind the primary ethylene response
CC element. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- MISCELLANEOUS: Loss-of-function mutations (EIL1-1 and EIL1-2) in the
CC gene show a weak ethylene-insensitive phenotype.
CC -!- SIMILARITY: Belongs to the EIN3 family. {ECO:0000305}.
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DR EMBL; AF004213; AAC49746.1; -; mRNA.
DR EMBL; AC005623; AAC77863.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07930.1; -; Genomic_DNA.
DR EMBL; AY065191; AAL38367.1; -; mRNA.
DR EMBL; BT003344; AAO29962.1; -; mRNA.
DR PIR; B84668; B84668.
DR RefSeq; NP_180273.1; NM_128263.5.
DR AlphaFoldDB; Q9SLH0; -.
DR SMR; Q9SLH0; -.
DR BioGRID; 2599; 14.
DR DIP; DIP-34866N; -.
DR IntAct; Q9SLH0; 21.
DR STRING; 3702.AT2G27050.1; -.
DR PaxDb; Q9SLH0; -.
DR PRIDE; Q9SLH0; -.
DR ProteomicsDB; 224495; -.
DR EnsemblPlants; AT2G27050.1; AT2G27050.1; AT2G27050.
DR GeneID; 817247; -.
DR Gramene; AT2G27050.1; AT2G27050.1; AT2G27050.
DR KEGG; ath:AT2G27050; -.
DR Araport; AT2G27050; -.
DR TAIR; locus:2059247; AT2G27050.
DR eggNOG; ENOG502QQSG; Eukaryota.
DR HOGENOM; CLU_027306_0_0_1; -.
DR InParanoid; Q9SLH0; -.
DR OMA; MDFFGAF; -.
DR OrthoDB; 266873at2759; -.
DR PhylomeDB; Q9SLH0; -.
DR PRO; PR:Q9SLH0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SLH0; baseline and differential.
DR Genevisible; Q9SLH0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009970; P:cellular response to sulfate starvation; IMP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IGI:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0009723; P:response to ethylene; IMP:TAIR.
DR Gene3D; 1.10.3180.10; -; 2.
DR InterPro; IPR006957; EIN3.
DR InterPro; IPR023278; Ethylene_insens-like_DNA-bd.
DR PANTHER; PTHR33305; PTHR33305; 2.
DR Pfam; PF04873; EIN3; 1.
DR SUPFAM; SSF116768; SSF116768; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; DNA-binding; Ethylene signaling pathway; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..584
FT /note="ETHYLENE INSENSITIVE 3-like 1 protein"
FT /id="PRO_0000113499"
FT REGION 67..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 41..74
FT /evidence="ECO:0000255"
FT CONFLICT 74
FT /note="E -> G (in Ref. 1; AAC49746)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 584 AA; 66495 MW; 471E1715D1483B11 CRC64;
MMMFNEMGMY GNMDFFSSST SLDVCPLPQA EQEPVVEDVD YTDDEMDVDE LEKRMWRDKM
RLKRLKEQQS KCKEGVDGSK QRQSQEQARR KKMSRAQDGI LKYMLKMMEV CKAQGFVYGI
IPEKGKPVTG ASDNLREWWK DKVRFDRNGP AAIAKYQSEN NISGGSNDCN SLVGPTPHTL
QELQDTTLGS LLSALMQHCD PPQRRFPLEK GVSPPWWPNG NEEWWPQLGL PNEQGPPPYK
KPHDLKKAWK VGVLTAVIKH MSPDIAKIRK LVRQSKCLQD KMTAKESATW LAIINQEEVV
ARELYPESCP PLSSSSSLGS GSLLINDCSE YDVEGFEKEQ HGFDVEERKP EIVMMHPLAS
FGVAKMQHFP IKEEVATTVN LEFTRKRKQN NDMNVMVMDR SAGYTCENGQ CPHSKMNLGF
QDRSSRDNHQ MVCPYRDNRL AYGASKFHMG GMKLVVPQQP VQPIDLSGVG VPENGQKMIT
ELMAMYDRNV QSNQTPPTLM ENQSMVIDAK AAQNQQLNFN SGNQMFMQQG TNNGVNNRFQ
MVFDSTPFDM AAFDYRDDWQ TGAMEGMGKQ QQQQQQQQDV SIWF