ID   EIL2_ARATH              Reviewed;         518 AA.
AC   O23115;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=ETHYLENE INSENSITIVE 3-like 2 protein;
GN   Name=EIL2; OrderedLocusNames=At5g21120; ORFNames=T10F18.150;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=cv. Columbia;
RX   PubMed=9215635; DOI=10.1016/s0092-8674(00)80300-1;
RA   Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R.;
RT   "Activation of the ethylene gas response pathway in Arabidopsis by the
RT   nuclear protein ETHYLENE-INSENSITIVE3 and related proteins.";
RL   Cell 89:1133-1144(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   CHARACTERIZATION, AND FUNCTION.
RX   PubMed=9851977; DOI=10.1101/gad.12.23.3703;
RA   Solano R., Stepanova A.N., Chao Q., Ecker J.R.;
RT   "Nuclear events in ethylene signaling: a transcriptional cascade mediated
RT   by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.";
RL   Genes Dev. 12:3703-3714(1998).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=12606727; DOI=10.1073/pnas.0438070100;
RA   Alonso J.M., Stepanova A.N., Solano R., Wisman E., Ferrari S.,
RA   Ausubel F.M., Ecker J.R.;
RT   "Five components of the ethylene-response pathway identified in a screen
RT   for weak ethylene-insensitive mutants in Arabidopsis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:2992-2997(2003).
CC   -!- FUNCTION: Probable transcription factor acting as a positive regulator
CC       in the ethylene response pathway. Could bind the primary ethylene
CC       response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter.
CC       {ECO:0000269|PubMed:9215635, ECO:0000269|PubMed:9851977}.
CC   -!- SUBUNIT: Acts as homodimer to bind the primary ethylene response
CC       element. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EIN3 family. {ECO:0000305}.
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DR   EMBL; AF004214; AAC49747.1; -; mRNA.
DR   EMBL; AC140977; AAO73887.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED92936.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM70600.1; -; Genomic_DNA.
DR   RefSeq; NP_001318615.1; NM_001343697.1.
DR   RefSeq; NP_197611.1; NM_122120.2.
DR   AlphaFoldDB; O23115; -.
DR   SMR; O23115; -.
DR   BioGRID; 17512; 31.
DR   IntAct; O23115; 30.
DR   STRING; 3702.AT5G21120.1; -.
DR   PaxDb; O23115; -.
DR   PRIDE; O23115; -.
DR   ProteomicsDB; 220443; -.
DR   EnsemblPlants; AT5G21120.1; AT5G21120.1; AT5G21120.
DR   EnsemblPlants; AT5G21120.3; AT5G21120.3; AT5G21120.
DR   GeneID; 832237; -.
DR   Gramene; AT5G21120.1; AT5G21120.1; AT5G21120.
DR   Gramene; AT5G21120.3; AT5G21120.3; AT5G21120.
DR   KEGG; ath:AT5G21120; -.
DR   Araport; AT5G21120; -.
DR   TAIR; locus:2178988; AT5G21120.
DR   eggNOG; ENOG502QQSG; Eukaryota.
DR   HOGENOM; CLU_027306_2_0_1; -.
DR   InParanoid; O23115; -.
DR   PhylomeDB; O23115; -.
DR   PRO; PR:O23115; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; O23115; baseline and differential.
DR   Genevisible; O23115; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3180.10; -; 1.
DR   InterPro; IPR006957; EIN3.
DR   InterPro; IPR023278; Ethylene_insens-like_DNA-bd.
DR   PANTHER; PTHR33305; PTHR33305; 1.
DR   Pfam; PF04873; EIN3; 1.
DR   SUPFAM; SSF116768; SSF116768; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; DNA-binding; Ethylene signaling pathway; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..518
FT                   /note="ETHYLENE INSENSITIVE 3-like 2 protein"
FT                   /id="PRO_0000113500"
FT   REGION          450..518
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          37..73
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        472..488
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        496..518
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   518 AA;  59186 MW;  173EA49BE9A17689 CRC64;
     MDMYNNNIGM FRSLVCSSAP PFTEGHMCSD SHTALCDDLS SDEEMEIEEL EKKIWRDKQR
     LKRLKEMAKN GLGTRLLLKQ QHDDFPEHSS KRTMYKAQDG ILKYMSKTME RYKAQGFVYG
     IVLENGKTVA GSSDNLREWW KDKVRFDRNG PAAIIKHQRD INLSDGSDSG SEVGDSTAQK
     LLELQDTTLG ALLSALFPHC NPPQRRFPLE KGVTPPWWPT GKEDWWDQLS LPVDFRGVPP
     PYKKPHDLKK LWKIGVLIGV IRHMASDISN IPNLVRRSRS LQEKMTSREG ALWLAALYRE
     KAIVDQIAMS RENNNTSNFL VPATGGDPDV LFPESTDYDV ELIGGTHRTN QQYPEFENNY
     NCVYKRKFEE DFGMPMHPTL LTCENSLCPY SQPHMGFLDR NLRENHQMTC PYKVTSFYQP
     TKPYGMTGLM VPCPDYNGMQ QQVQSFQDQF NHPNDLYRPK APQRGNDDLV EDLNPSPSTL
     NQNLGLVLPT DFNGGEETVG TENNLHNQGQ ELPTSWIQ