EIL3_ARATH
ID EIL3_ARATH Reviewed; 567 AA.
AC O23116;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=ETHYLENE INSENSITIVE 3-like 3 protein;
GN Name=EIL3; OrderedLocusNames=At1g73730; ORFNames=F25P22.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=9215635; DOI=10.1016/s0092-8674(00)80300-1;
RA Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R.;
RT "Activation of the ethylene gas response pathway in Arabidopsis by the
RT nuclear protein ETHYLENE-INSENSITIVE3 and related proteins.";
RL Cell 89:1133-1144(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP CHARACTERIZATION, AND FUNCTION.
RX PubMed=9851977; DOI=10.1101/gad.12.23.3703;
RA Solano R., Stepanova A.N., Chao Q., Ecker J.R.;
RT "Nuclear events in ethylene signaling: a transcriptional cascade mediated
RT by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.";
RL Genes Dev. 12:3703-3714(1998).
RN [6]
RP STRUCTURE BY NMR OF 162-288.
RX PubMed=15811366; DOI=10.1016/j.jmb.2005.02.065;
RA Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki T., Aoki M., Seki E.,
RA Matsuda T., Tomo Y., Terada T., Shirouzu M., Tanaka A., Seki M.,
RA Shinozaki K., Yokoyama S.;
RT "Solution structure of the major DNA-binding domain of Arabidopsis thaliana
RT ethylene-insensitive3-like3.";
RL J. Mol. Biol. 348:253-264(2005).
RN [7]
RP INTERACTION WITH MYB72.
RC STRAIN=cv. Columbia;
RX PubMed=18218967; DOI=10.1104/pp.107.113829;
RA Van der Ent S., Verhagen B.W.M., Van Doorn R., Bakker D., Verlaan M.G.,
RA Pel M.J.C., Joosten R.G., Proveniers M.C.G., Van Loon L.C., Ton J.,
RA Pieterse C.M.J.;
RT "MYB72 is required in early signaling steps of rhizobacteria-induced
RT systemic resistance in Arabidopsis.";
RL Plant Physiol. 146:1293-1304(2008).
CC -!- FUNCTION: Probable transcription factor that may be involved in the
CC ethylene response pathway. {ECO:0000269|PubMed:9215635,
CC ECO:0000269|PubMed:9851977}.
CC -!- SUBUNIT: Interacts with MYB72. {ECO:0000269|PubMed:18218967}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EIN3 family. {ECO:0000305}.
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DR EMBL; AF004215; AAC49748.1; -; mRNA.
DR EMBL; AC012679; AAG52067.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35502.1; -; Genomic_DNA.
DR EMBL; AY070044; AAL49801.1; -; mRNA.
DR EMBL; AY133839; AAM91773.1; -; mRNA.
DR PIR; E96764; E96764.
DR RefSeq; NP_177514.1; NM_106032.5.
DR PDB; 1WIJ; NMR; -; A=162-288.
DR PDBsum; 1WIJ; -.
DR AlphaFoldDB; O23116; -.
DR SMR; O23116; -.
DR BioGRID; 28927; 4.
DR IntAct; O23116; 3.
DR STRING; 3702.AT1G73730.1; -.
DR iPTMnet; O23116; -.
DR PaxDb; O23116; -.
DR PRIDE; O23116; -.
DR ProteomicsDB; 222674; -.
DR EnsemblPlants; AT1G73730.1; AT1G73730.1; AT1G73730.
DR GeneID; 843708; -.
DR Gramene; AT1G73730.1; AT1G73730.1; AT1G73730.
DR KEGG; ath:AT1G73730; -.
DR Araport; AT1G73730; -.
DR TAIR; locus:2027754; AT1G73730.
DR eggNOG; ENOG502QQCD; Eukaryota.
DR HOGENOM; CLU_027306_1_1_1; -.
DR InParanoid; O23116; -.
DR OrthoDB; 674199at2759; -.
DR PhylomeDB; O23116; -.
DR EvolutionaryTrace; O23116; -.
DR PRO; PR:O23116; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O23116; baseline and differential.
DR Genevisible; O23116; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009970; P:cellular response to sulfate starvation; IMP:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0042762; P:regulation of sulfur metabolic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 1.10.3180.10; -; 2.
DR InterPro; IPR006957; EIN3.
DR InterPro; IPR023278; Ethylene_insens-like_DNA-bd.
DR PANTHER; PTHR33305; PTHR33305; 1.
DR Pfam; PF04873; EIN3; 1.
DR SUPFAM; SSF116768; SSF116768; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; DNA-binding; Ethylene signaling pathway;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..567
FT /note="ETHYLENE INSENSITIVE 3-like 3 protein"
FT /id="PRO_0000113501"
FT DNA_BIND 162..288
FT REGION 55..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 24..44
FT /evidence="ECO:0000255"
FT COMPBIAS 286..302
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..318
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 374..388
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 171..181
FT /evidence="ECO:0007829|PDB:1WIJ"
FT STRAND 182..187
FT /evidence="ECO:0007829|PDB:1WIJ"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 199..202
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 209..214
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 232..245
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 247..249
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 250..255
FT /evidence="ECO:0007829|PDB:1WIJ"
FT TURN 256..259
FT /evidence="ECO:0007829|PDB:1WIJ"
FT STRAND 260..263
FT /evidence="ECO:0007829|PDB:1WIJ"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:1WIJ"
FT HELIX 270..279
FT /evidence="ECO:0007829|PDB:1WIJ"
FT TURN 280..284
FT /evidence="ECO:0007829|PDB:1WIJ"
SQ SEQUENCE 567 AA; 64042 MW; 308AFEE4B3109594 CRC64;
MGDLAMSVAD IRMENEPDDL ASDNVAEIDV SDEEIDADDL ERRMWKDRVR LKRIKERQKA
GSQGAQTKET PKKISDQAQR KKMSRAQDGI LKYMLKLMEV CKVRGFVYGI IPEKGKPVSG
SSDNIRAWWK EKVKFDKNGP AAIAKYEEEC LAFGKSDGNR NSQFVLQDLQ DATLGSLLSS
LMQHCDPPQR KYPLEKGTPP PWWPTGNEEW WVKLGLPKSQ SPPYRKPHDL KKMWKVGVLT
AVINHMLPDI AKIKRHVRQS KCLQDKMTAK ESAIWLAVLN QEESLIQQPS SDNGNSNVTE
THRRGNNADR RKPVVNSDSD YDVDGTEEAS GSVSSKDSRR NQIQKEQPTA ISHSVRDQDK
AEKHRRRKRP RIRSGTVNRQ EEEQPEAQQR NILPDMNHVD APLLEYNING THQEDDVVDP
NIALGPEDNG LELVVPEFNN NYTYLPLVNE QTMMPVDERP MLYGPNPNQE LQFGSGYNFY
NPSAVFVHNQ EDDILHTQIE MNTQAPPHNS GFEEAPGGVL QPLGLLGNED GVTGSELPQY
QSGILSPLTD LDFDYGGFGD DFSWFGA
