EIN2_ORYSJ
ID EIN2_ORYSJ Reviewed; 1281 AA.
AC Q0D8I9; Q69QL2; Q69QM8; Q6TJY0; Q6Z120;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Protein ETHYLENE-INSENSITIVE 2 {ECO:0000305};
DE Short=OsEIN2 {ECO:0000303|PubMed:15047876};
DE AltName: Full=Protein MAO HUZI 7 {ECO:0000303|PubMed:23718947};
GN Name=EIN2 {ECO:0000303|PubMed:15047876};
GN Synonyms=MHZ7 {ECO:0000303|PubMed:23718947};
GN OrderedLocusNames=Os07g0155600 {ECO:0000312|EMBL:BAF20834.1},
GN LOC_Os07g06130 {ECO:0000305};
GN ORFNames=OSJNBa0024L18.19 {ECO:0000312|EMBL:BAD31350.1},
GN OSJNBa0024L18.30 {ECO:0000312|EMBL:BAD31359.1},
GN OSJNBa0024L18.49 {ECO:0000312|EMBL:BAD31367.1},
GN P0455F03.4 {ECO:0000312|EMBL:BAC84320.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15047876; DOI=10.1093/pcp/pch033;
RA Jun S.H., Han M.J., Lee S., Seo Y.S., Kim W.T., An G.;
RT "OsEIN2 is a positive component in ethylene signaling in rice.";
RL Plant Cell Physiol. 45:281-289(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23718947; DOI=10.1093/mp/sst087;
RA Ma B., He S.J., Duan K.X., Yin C.C., Chen H., Yang C., Xiong Q., Song Q.X.,
RA Lu X., Chen H.W., Zhang W.K., Lu T.G., Chen S.Y., Zhang J.S.;
RT "Identification of rice ethylene-response mutants and characterization of
RT MHZ7/OsEIN2 in distinct ethylene response and yield trait regulation.";
RL Mol. Plant 6:1830-1848(2013).
CC -!- FUNCTION: Central factor in ethylene signaling pathways that control
CC development, senescence and grain size. Acts as positive component of
CC the ethylene-signaling pathway. {ECO:0000269|PubMed:15047876,
CC ECO:0000269|PubMed:23718947}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf sheaths, leaf blades,
CC flowers, developing seeds, germinating seeds and young seedlings
CC (PubMed:15047876). Expressed in adventitious roots, vascular tissues of
CC the seminal roots, lateral roots, the connecting region between
CC vascular tissues and lateral roots, mature leaf, mature stem, tips of
CC adventitious roots derived from the node, shoot apex, young panicle,
CC anthers, pistil, stigma, ovary, seed coat and fruit coat pericarp.
CC {ECO:0000269|PubMed:15047876}.
CC -!- DISRUPTION PHENOTYPE: Mutant etiolated seedlings display insensitivite
CC ethylene-response in roots and coleoptiles. Mature mutant plants have
CC reduced shoot length, increased number of panicles and reduced grain
CC yield. {ECO:0000269|PubMed:23718947}.
CC -!- MISCELLANEOUS: Plants silencing EIN2 display a stunted phenotype with
CC severe inhibition of shoot elongation and produce high levels of
CC ethylene (PubMed:15047876). 'Mao huzi' means cat whiskers in Chinese
CC (PubMed:23718947). {ECO:0000269|PubMed:15047876,
CC ECO:0000303|PubMed:23718947}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC84320.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD31350.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD31359.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD31367.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY396568; AAQ95276.1; -; mRNA.
DR EMBL; AP005409; BAD31350.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005409; BAD31359.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005409; BAD31367.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005454; BAC84320.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008213; BAF20834.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00121.1; -; Genomic_DNA.
DR RefSeq; XP_015646967.1; XM_015791481.1.
DR AlphaFoldDB; Q0D8I9; -.
DR SMR; Q0D8I9; -.
DR STRING; 4530.OS07T0155600-01; -.
DR PaxDb; Q0D8I9; -.
DR PRIDE; Q0D8I9; -.
DR EnsemblPlants; Os07t0155600-01; Os07t0155600-01; Os07g0155600.
DR GeneID; 4342431; -.
DR Gramene; Os07t0155600-01; Os07t0155600-01; Os07g0155600.
DR KEGG; osa:4342431; -.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_006509_0_0_1; -.
DR InParanoid; Q0D8I9; -.
DR OMA; QMNANRN; -.
DR OrthoDB; 666470at2759; -.
DR PlantReactome; R-OSA-5225756; Ethylene mediated signaling.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:UniProtKB.
DR InterPro; IPR017187; EIN2.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PIRSF; PIRSF037378; EIN2; 1.
DR PRINTS; PR00447; NATRESASSCMP.
PE 2: Evidence at transcript level;
KW Ethylene signaling pathway; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..1281
FT /note="Protein ETHYLENE-INSENSITIVE 2"
FT /id="PRO_0000435899"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..61
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..137
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..208
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..297
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..362
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..447
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..1281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 540..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 593..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 599..627
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 631..647
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 633
FT /note="P -> S (in Ref. 1; AAQ95276)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1281 AA; 138214 MW; 3D698D72D1B91C19 CRC64;
MDGQQLRSSE SPASGGGGVT GGGAPHLFHA LGPALLISIG YIDLGKWVAA VEAGSRFGLD
LVLLALLFNF MAILCQYLAA CIGTVTGRSL AEICHQEYSR PTCIFLGVQA GLSLLTSELT
MIFGIALGFN LLFEYDDLIT GICFATVVPN LLPYAISHLG KKMVGTLNAC IAGFALLCYV
LGLLVSQPQI PLTTNVIFPK LSGESAYSLM ALLGANVMAH NFYIHSSVVQ GQKRSAFAVG
ALFHDHLFSV LFIFTGIFLV NHVLMNSAAA DSTNTLLLTF QDVVELMNQI FVNPMAPTIF
LVVLLFSSHI ISLTSAIGSQ VISQHLFGIN LPLSGHHLIL KAFAIVPALY CAKVAGAEGI
YQLLIICQII QAMLLPSSVV PLFRVASSRL IMGAHRVSLH LEILTFLAFL LMLFSNIIFM
AEMLFGDSGW LNTLKGNTGS PVVFPSTVLI TVACVSVAFS LYMAVTPLKS GSHEAELQQE
WSVPSQKELL NTTQDREETC AGNVTYEEDQ RSDVVPSPRI QPVDCLKSAL DYIDSSDTAI
ESDHDSQHST AHTSTAPESC HSPSFIPEES KSVVAVDWPE PLEPISNAIV AEESTVESVD
SKSTGERDIE VEPALLMDND KEAPNILESD NKPLGGNNPS CASDDGPPSL TFSRGKGSDA
GNGSGSLSRL SGLGRAARRQ LAAILDEFWG HLFDYHGKLT QEASSKRFDI LLGLDVRTPS
STVRADSQAN EIPKSPMVRD NLQGSAFLGS SRDLMSTKNE MSNLDLTYGL QMGNNIGSSA
WSQGMQLPST QLQSSSNSLL DQGARLNSNF STPSYADNNQ FYQPATIHGY QLASYLKQMN
ANRNPYSSMP LDPQRLPKSS ASAVPTYVDS VMHARNQNLL ASLGATPSQI AATSRIGTMM
AERSYYVPST LDGNENAGSS AYSKKYHSSP DISALIAASR SALLNESKLG GGTIGSQSYL
SRLASERSQY TNSVARPAAP LAFDELSPPK LPGDIFSMQQ SPNPSARSLW AKQPFEQLFG
VSSAELTKSE FNPAGRSGGM TKDDFSYKES EAKLLQSLRF CISKLLKLEG SGWLFKQNGG
SDEDLIDQVA AVEKLLQQGT SDNQLLLGDT QQPPCDKADI QYMRVLPNCG DDCIWRASLV
VSFGVWCIRR VLDLSLVESR PELWGKYTYV LNRLQGILDP AFSKPRSALS ACACLHRDIR
VLNSLRHSSL VATNSIPRQI RGSFTTASVV LEMIKDVETA VSGRKGRSGT AAGDVAFPKG
KENLASVLKR YKRRLSSKGQ Q
