EIPR1_MOUSE
ID EIPR1_MOUSE Reviewed; 386 AA.
AC Q8K0G5; E9QN80;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=EARP and GARP complex-interacting protein 1 {ECO:0000250|UniProtKB:Q53HC9};
DE AltName: Full=Endosome-associated recycling protein-interacting protein {ECO:0000250|UniProtKB:Q53HC9};
DE AltName: Full=Golgi-associated retrograde protein-interacting protein {ECO:0000250|UniProtKB:Q53HC9};
DE AltName: Full=Tumor-suppressing STF cDNA 1 protein;
DE AltName: Full=Tumor-suppressing subchromosomal transferable fragment candidate gene 1 protein {ECO:0000250|UniProtKB:Q53HC9};
GN Name=Eipr1 {ECO:0000312|MGI:MGI:1289332};
GN Synonyms=Tssc1 {ECO:0000312|MGI:MGI:1289332};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as a component of endosomal retrieval machinery that is
CC involved in protein transport from early endosomes to either recycling
CC endosomes or the trans-Golgi network (By similarity). Mediates the
CC recruitment of Golgi-associated retrograde protein (GARP) complex to
CC the trans-Golgi network and controls early endosome-to-Golgi transport
CC of internalized protein (By similarity). Promotes the recycling of
CC internalized transferrin receptor (TFRC) to the plasma membrane through
CC interaction with endosome-associated recycling protein (EARP) complex
CC (By similarity). Controls proper insulin distribution and secretion,
CC and retention of cargo in mature dense core vesicles (By similarity).
CC Required for the stability of the endosome-associated retrograde
CC protein (EARP) complex subunits and for proper localization and
CC association of EARP with membranes (By similarity).
CC {ECO:0000250|UniProtKB:Q53HC9, ECO:0000250|UniProtKB:Q5PPK9}.
CC -!- SUBUNIT: Interacts with two multisubunit tethering complexes: EARP
CC composed of VPS50, VPS51, VPS52 and VPS53 subunits and GARP complex
CC composed of VPS51, VPS52, VPS53 and VPS54 subunits. Interacts with
CC SNAP29. {ECO:0000250|UniProtKB:Q53HC9}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250|UniProtKB:Q53HC9}.
CC -!- SIMILARITY: Belongs to the WD repeat EIPR1 family. {ECO:0000305}.
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DR EMBL; AC136986; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT010449; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC031458; AAH31458.1; -; mRNA.
DR CCDS; CCDS25855.1; -.
DR RefSeq; NP_958745.2; NM_201357.2.
DR AlphaFoldDB; Q8K0G5; -.
DR SMR; Q8K0G5; -.
DR BioGRID; 237632; 14.
DR STRING; 10090.ENSMUSP00000038845; -.
DR iPTMnet; Q8K0G5; -.
DR PhosphoSitePlus; Q8K0G5; -.
DR SwissPalm; Q8K0G5; -.
DR EPD; Q8K0G5; -.
DR MaxQB; Q8K0G5; -.
DR PaxDb; Q8K0G5; -.
DR PeptideAtlas; Q8K0G5; -.
DR PRIDE; Q8K0G5; -.
DR ProteomicsDB; 277849; -.
DR Antibodypedia; 26290; 194 antibodies from 26 providers.
DR DNASU; 380752; -.
DR Ensembl; ENSMUST00000221877; ENSMUSP00000152220; ENSMUSG00000036613.
DR GeneID; 380752; -.
DR KEGG; mmu:380752; -.
DR UCSC; uc007ngb.2; mouse.
DR CTD; 7260; -.
DR MGI; MGI:1289332; Eipr1.
DR VEuPathDB; HostDB:ENSMUSG00000036613; -.
DR eggNOG; KOG1007; Eukaryota.
DR GeneTree; ENSGT00730000111137; -.
DR HOGENOM; CLU_050772_0_0_1; -.
DR InParanoid; Q8K0G5; -.
DR OMA; HQFLALH; -.
DR OrthoDB; 814890at2759; -.
DR PhylomeDB; Q8K0G5; -.
DR TreeFam; TF105847; -.
DR BioGRID-ORCS; 380752; 15 hits in 73 CRISPR screens.
DR ChiTaRS; Tssc1; mouse.
DR PRO; PR:Q8K0G5; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8K0G5; protein.
DR Bgee; ENSMUSG00000036613; Expressed in embryonic brain and 240 other tissues.
DR ExpressionAtlas; Q8K0G5; baseline and differential.
DR Genevisible; Q8K0G5; MM.
DR GO; GO:1990745; C:EARP complex; ISO:MGI.
DR GO; GO:0000938; C:GARP complex; ISO:MGI.
DR GO; GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
DR GO; GO:0032456; P:endocytic recycling; ISS:UniProtKB.
DR GO; GO:1904811; P:positive regulation of dense core granule transport; ISS:UniProtKB.
DR GO; GO:2001137; P:positive regulation of endocytic recycling; ISS:UniProtKB.
DR GO; GO:1905281; P:positive regulation of retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR040323; EIPR1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR PANTHER; PTHR14205; PTHR14205; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 5.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; Golgi apparatus; Phosphoprotein; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..386
FT /note="EARP and GARP complex-interacting protein 1"
FT /id="PRO_0000051300"
FT REPEAT 132..172
FT /note="WD 1"
FT REPEAT 182..222
FT /note="WD 2"
FT REPEAT 226..266
FT /note="WD 3"
FT REPEAT 270..310
FT /note="WD 4"
FT REPEAT 344..384
FT /note="WD 5"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q53HC9"
FT MOD_RES 320
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q53HC9"
FT CONFLICT 317..319
FT /note="GDI -> DDV (in Ref. 2; AAH31458)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 386 AA; 43127 MW; D555D19FB7D700F4 CRC64;
MEDDAPVIYG LEFQARALTP QTAETDAIRF LVGTQSLKYD NQIHIIDFDD ENNIINKNVL
LHQAGEIWHI SASPADKGVL ATCYNKTTDS RVQACAAVWR MPKELESGSH ESPDDPASTA
QTLELLCHLD NSAQGNVACV VWEPMGDGKK VISLADSHIL LWDLQPSSSQ AVLASSATLE
GRGQLKFTAG RWSPHHNCTQ VATASDTTLR GWDTRSMSQI YCIENAHGQL VRDLDFNPNK
QYYLASCGDD CKVKFWDTRN VTEPVKTLEE HSHWVWSVRY NHSHDQLVLT GSSDSRVILS
NMVSISSEPF GHLVDDGDIS DPEEHHAEKS KEPLQDNVIA TYEEHEDSVY AVDWASADPW
LFASLSYDGR LVINRVPRAL KYHILL
