AGAL1_PEDPE
ID AGAL1_PEDPE Reviewed; 733 AA.
AC P43467;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Alpha-galactosidase 1;
DE EC=3.2.1.22;
DE AltName: Full=Melibiase;
GN Name=agaR;
OS Pediococcus pentosaceus.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Pediococcus.
OX NCBI_TaxID=1255;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PPE1.0;
RA Leenhouts K.K.J., Bolhuis A.A., Kok J.J., Venema G.G.;
RT "The sucrose and raffinose operons of Pediococcus pentosaceus PPE1.0.";
RL Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Alpha-galactosidase associated with the raffinose operon.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family. {ECO:0000305}.
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DR EMBL; Z32771; CAA83665.1; -; Genomic_DNA.
DR EMBL; L32093; AAA25564.1; -; Genomic_DNA.
DR PIR; S44254; S44254.
DR RefSeq; WP_056979569.1; NZ_WEOZ01000006.1.
DR AlphaFoldDB; P43467; -.
DR SMR; P43467; -.
DR CAZy; GH36; Glycoside Hydrolase Family 36.
DR GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 2.70.98.60; -; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase; Hydrolase.
FT CHAIN 1..733
FT /note="Alpha-galactosidase 1"
FT /id="PRO_0000134874"
FT ACT_SITE 480
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9ALJ4"
FT ACT_SITE 550
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q9ALJ4"
SQ SEQUENCE 733 AA; 83314 MW; 7732ACFDB655EE88 CRC64;
MSLITVDQAN RVFHLHNQTL SYIFAVEQGG TLSHLYFGGH VDHYHGELRY PRVDRGFSGN
LPGSTDRTFS RDTLPKEYST AGEMDYHLPA AIVRHTDGAN ALYLVYQGYR IEAGKPKLSG
LPAAFVEDET EAETLTIVLV DQVSQVEFDL QYTIYRDRPV VTRSVQVCNQ GDHAVNLEKV
ASMQIDFTDR QFETITLPGA HANERHPERG SINYGIQTFG SLRGTSSHQM NPFLALVDHT
TTEFSGDAYG FNLVYSGNHA FELEKDQLDQ LHLMVGINSY NFNWQLKAGA TFQTPEVLMV
YTNKGLNAMS QAYHHLIRER VVRSEFKNQE RPIVVNNWEA TFFDFNEAKL KPIVDEAKQL
GIEMFVLDDG WFGHRDDDNS SLGDWQVDHR KFPQGLNHFV KYVHEQGLKF GIWLEPEMIS
YDSKLYQQHP DYLMQVPGRS PSPSRNQYIL DLGRQAVRNN IFDQLDQLLK SKQIDYIKWD
MNRHLSDIYS VALPPERQGE VYHRYVLGLY ELLERLTTAY PHILFEGCSG GGGRFDAGMA
YYMPQIWASD NTDAVARLTI QYGTSLAYPI SLATAHVSVS PNQQTGRETS MSTRSAVAAS
GVLGYELDLT QLSSADKQIV QKQVVQYKQI RPLIQFGEFY RLKSPITSNQ AAWMFVSPQQ
DEAIVMVFNL TSYAQPSLTK TKLVGLNPKL NYQNIATKAI FGGDELMQLG FYDPVVYQDY
TTKVYHFKAV TEN