EKI_ARATH
ID EKI_ARATH Reviewed; 374 AA.
AC O81024; Q8LAQ2;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Probable ethanolamine kinase;
DE EC=2.7.1.82;
DE AltName: Full=Protein EMBRYO DEFECTIVE 1187;
GN Name=EMB1187; OrderedLocusNames=At2g26830; ORFNames=F12C20.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in phospholipid biosynthesis. Catalyzes the first
CC step in phosphatidylethanolamine biosynthesis (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + ethanolamine = ADP + H(+) + phosphoethanolamine;
CC Xref=Rhea:RHEA:13069, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57603, ChEBI:CHEBI:58190, ChEBI:CHEBI:456216;
CC EC=2.7.1.82;
CC -!- PATHWAY: Phospholipid metabolism; phosphatidylethanolamine
CC biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
CC -!- SIMILARITY: Belongs to the choline/ethanolamine kinase family.
CC {ECO:0000305}.
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DR EMBL; AC005168; AAC32242.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07895.1; -; Genomic_DNA.
DR EMBL; AK229423; BAF01284.1; -; mRNA.
DR EMBL; AY087681; AAM65218.1; -; mRNA.
DR PIR; T02652; T02652.
DR RefSeq; NP_180251.1; NM_128240.4.
DR AlphaFoldDB; O81024; -.
DR SMR; O81024; -.
DR STRING; 3702.AT2G26830.1; -.
DR PaxDb; O81024; -.
DR PRIDE; O81024; -.
DR ProteomicsDB; 220657; -.
DR DNASU; 817224; -.
DR EnsemblPlants; AT2G26830.1; AT2G26830.1; AT2G26830.
DR GeneID; 817224; -.
DR Gramene; AT2G26830.1; AT2G26830.1; AT2G26830.
DR KEGG; ath:AT2G26830; -.
DR Araport; AT2G26830; -.
DR TAIR; locus:2039518; AT2G26830.
DR eggNOG; KOG4720; Eukaryota.
DR HOGENOM; CLU_012712_0_0_1; -.
DR InParanoid; O81024; -.
DR OMA; FKMKPEA; -.
DR OrthoDB; 349250at2759; -.
DR PhylomeDB; O81024; -.
DR BioCyc; ARA:AT2G26830-MON; -.
DR BRENDA; 2.7.1.82; 399.
DR UniPathway; UPA00558; UER00741.
DR PRO; PR:O81024; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O81024; baseline and differential.
DR Genevisible; O81024; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004305; F:ethanolamine kinase activity; IBA:GO_Central.
DR GO; GO:0006646; P:phosphatidylethanolamine biosynthetic process; IBA:GO_Central.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IMP:TAIR.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR SUPFAM; SSF56112; SSF56112; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Lipid biosynthesis; Lipid metabolism;
KW Nucleotide-binding; Phospholipid biosynthesis; Phospholipid metabolism;
KW Reference proteome; Transferase.
FT CHAIN 1..374
FT /note="Probable ethanolamine kinase"
FT /id="PRO_0000423349"
FT BINDING 93
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 252
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 150
FT /note="P -> Q (in Ref. 4; AAM65218)"
FT /evidence="ECO:0000305"
FT CONFLICT 211
FT /note="K -> T (in Ref. 4; AAM65218)"
FT /evidence="ECO:0000305"
FT CONFLICT 374
FT /note="L -> M (in Ref. 4; AAM65218)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 374 AA; 42860 MW; 7E02759EA384DEDD CRC64;
MGAAKNIWAL ANAEDAANDA EQIPYSSFVV DTSLPLPLMI PRIIELCKDL FKNWGELDDS
LFSVERVSGG ITNLLLKVSV KEDTNKEVSV TVRLYGPNTE YVINREREIL AIKYLSAAGF
GAKLLGGFGN GMVQSFINAR TLEPSDMREP KIAAQIAREL GKFHKVDIPG SKEPQLWVDI
LKFYEKASTL TFEEPDKQKL FETISFEELH KEIIELREFT GLLNAPVVFA HNDLLSGNFM
LNDEEEKLYL IDFEYGSYNY RGFDIGNHFN EYAGYDCDYS LYPSKEEQYH FIKHYLQPDK
PDEVSIAEVE SVFVETDAYK LASHLYWAIW AIIQARMSPI EFEYLGYFFL RYNEYKKQKP
LTFSLVTSHL SASL
