ID   AGAL2_PEDPE             Reviewed;         719 AA.
AC   P43469;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Alpha-galactosidase 2;
DE            EC=3.2.1.22;
DE   AltName: Full=Melibiase;
GN   Name=agaS;
OS   Pediococcus pentosaceus.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Pediococcus.
OX   NCBI_TaxID=1255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PPE1.0;
RA   Leenhouts K.J., Bolhuis A.A., Kok J.J., Venema G.G.;
RT   "The sucrose and raffinose operons of Pediococcus pentosaceus PPE1.0.";
RL   Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Alpha-galactosidase associated with the sucrase operon.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family. {ECO:0000305}.
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DR   EMBL; L32093; AAA25566.1; -; Genomic_DNA.
DR   AlphaFoldDB; P43469; -.
DR   SMR; P43469; -.
DR   CAZy; GH36; Glycoside Hydrolase Family 36.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR   CDD; cd14791; GH36; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   Gene3D; 2.70.98.60; -; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR038417; Alpga-gal_N_sf.
DR   InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR   InterPro; IPR002252; Glyco_hydro_36.
DR   InterPro; IPR031705; Glyco_hydro_36_C.
DR   InterPro; IPR031704; Glyco_hydro_36_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF16874; Glyco_hydro_36C; 1.
DR   Pfam; PF16875; Glyco_hydro_36N; 1.
DR   PIRSF; PIRSF005536; Agal; 1.
DR   PRINTS; PR00743; GLHYDRLASE36.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..719
FT                   /note="Alpha-galactosidase 2"
FT                   /id="PRO_0000134875"
FT   ACT_SITE        472
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9ALJ4"
FT   ACT_SITE        542
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9ALJ4"
SQ   SEQUENCE   719 AA;  81368 MW;  CB03B93A5EC8A403 CRC64;
     MPVEYDPKTG LINLHNDQIS YVIQILAHRY PVHRYFGRYF SKQPYFEPMP SGSHAFANDP
     TERFPYSVTS LPLEYSTIGS GDYRQPAYVI KDANNQLLPI LEYTGFSVND QPINSRQLPP
     TVSKHTPVTT LVIHLTDAVT KLQMDLNYTI FENQPLILRS TTLRHHGTTN LQVTALSSAQ
     LDLPTDQYTA LTLSGTHAHE ANPSFNRLHP GLQTVRSLRG TSGPQHQPFM ALAEPNTTEL
     AGTVIGCALA WSGNFDSTVE VDQYQHSRLT IGLEPDTFEW QLKPNSSFQT PEAVLTWTNT
     GFNGMSQVFH DFSYQLMPSQ TNIPSVLNTW ETLTFAVSES KVQHLIEHAH QLGLQMLVLD
     DGWFVNRNGE NGQLGDWFVD PIKFPNGLNP LAQQAHHHRM KFGLWVEPEM ITTNSQLYQQ
     HPDWVLQYVD RTPITARHQL VLDLSQAAVR DHLITTLTNL VQNNQLDYLK WDMNRHLTQV
     GSTHLPAAQQ GELYHRYVCG LYDILTRLKR ACPKLIIENC SAGGGRFDFG MLPYTNQTWI
     SDLTDPVDRA TIENGFSYLF PPRIFSNHIT ASPNAQNGRI TPFETRLQLA CIGQLGLELN
     PKQLAPSEQQ LLRGALIKYQ QLKSTFIKAH FYRLPTTRHV VAWLIVTADK KQAICCYLNG
     LNSRVKTQHP LPLHYLDAEL AYSDSSGNRY TGHQLNTMGI PLKPTNADFT SQLIYLCQN