ID   ELAC_NOTEU              Reviewed;          83 AA.
AC   O62845;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Early lactation protein;
GN   Name=ELP;
OS   Notamacropus eugenii (Tammar wallaby) (Macropus eugenii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Diprotodontia; Macropodidae; Notamacropus.
OX   NCBI_TaxID=9315;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 1-30 AND 47-71.
RC   TISSUE=Lactating mammary gland;
RX   PubMed=9787813; DOI=10.1016/s0305-0491(98)10040-8;
RA   Simpson K.J., Shaw D., Nicholas K.R.;
RT   "Developmentally-regulated expression of a putative protease inhibitor gene
RT   in the lactating mammary gland of the tammar wallaby, Macropus eugenii.
RT   eugenii.";
RL   Comp. Biochem. Physiol. 120B:535-541(1998).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Found in the whey fraction of milk.
CC   -!- DEVELOPMENTAL STAGE: Expressed only in the early stage of lactation.
CC       Decrease in expression correlates with a change in the sucking pattern
CC       of the young.
CC   -!- PTM: N-glycosylated. {ECO:0000305}.
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DR   EMBL; AJ000490; CAA04128.1; -; mRNA.
DR   AlphaFoldDB; O62845; -.
DR   SMR; O62845; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007595; P:lactation; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Lactation;
KW   Milk protein; Protease inhibitor; Secreted; Serine protease inhibitor.
FT   CHAIN           1..83
FT                   /note="Early lactation protein"
FT                   /id="PRO_0000155448"
FT   DOMAIN          23..73
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            33..34
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        14
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        42
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        23..73
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        32..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        48..69
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   83 AA;  9583 MW;  CD94CD35EF3175E1 CRC64;
     EKCLDQIQVN SLENLSLLVP SLCLLPPVRG NCSSQILHYF YNTTSRTCET FIYSGCNGNR
     NNFNSEEYCL KTCRRNKNRN NNN