ID   ELAC_TRIVU              Reviewed;         102 AA.
AC   Q29143;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Early lactation protein;
DE   Flags: Precursor;
GN   Name=ELP;
OS   Trichosurus vulpecula (Brush-tailed possum).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Diprotodontia; Phalangeridae; Trichosurus.
OX   NCBI_TaxID=9337;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 21-30.
RC   TISSUE=Mammary gland;
RX   PubMed=8651704; DOI=10.1006/abbi.1996.0225;
RA   Piotte C.P., Grigor M.R.;
RT   "A novel marsupial protein expressed by the mammary gland only during the
RT   early lactation and related to the Kunitz proteinase inhibitors.";
RL   Arch. Biochem. Biophys. 330:59-64(1996).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the mammary gland.
CC   -!- DEVELOPMENTAL STAGE: Expressed only during early lactation phase.
CC   -!- PTM: N-glycosylated.
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DR   EMBL; U34208; AAB08977.1; -; mRNA.
DR   PIR; S69288; S69288.
DR   AlphaFoldDB; Q29143; -.
DR   SMR; Q29143; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007595; P:lactation; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Lactation;
KW   Milk protein; Protease inhibitor; Secreted; Serine protease inhibitor;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:8651704"
FT   CHAIN           21..102
FT                   /note="Early lactation protein"
FT                   /id="PRO_0000016878"
FT   DOMAIN          43..93
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            53..54
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        34
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        62
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        43..93
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        52..76
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        68..89
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   102 AA;  11407 MW;  D769CA327D2E1BD3 CRC64;
     MKFTIIALCL ALSLVGMTSS EKLLDRIRAN SLENLSRLVP SLCLLPSGRG NCDSQILRYF
     YNATSHTCEV FLYSGCNGNG NNFDSLECCL KTCRLNKYRN NN