ID   ELAD_ECO57              Reviewed;         407 AA.
AC   Q8XCY9; Q7AC13;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Protease ElaD;
DE            EC=3.4.22.-;
DE   AltName: Full=Deubiquitinase;
DE   AltName: Full=Deubiquitinating enzyme;
DE            Short=DUB;
DE   AltName: Full=Deubiquitinating protease;
GN   Name=elaD; OrderedLocusNames=Z3529, ECs3157;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Protease that can act as an efficient and specific
CC       deubiquitinating enzyme in vitro. Does not possess desumoylating and
CC       deneddylating activities. The physiological substrate is unknown (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C79 family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG57402.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36580.1; -; Genomic_DNA.
DR   PIR; E91023; E91023.
DR   PIR; F85867; F85867.
DR   RefSeq; NP_311184.1; NC_002695.1.
DR   AlphaFoldDB; Q8XCY9; -.
DR   SMR; Q8XCY9; -.
DR   STRING; 155864.EDL933_3436; -.
DR   MEROPS; C79.001; -.
DR   EnsemblBacteria; AAG57402; AAG57402; Z3529.
DR   EnsemblBacteria; BAB36580; BAB36580; ECs_3157.
DR   GeneID; 916865; -.
DR   KEGG; ece:Z3529; -.
DR   KEGG; ecs:ECs_3157; -.
DR   PATRIC; fig|386585.9.peg.3295; -.
DR   eggNOG; COG5160; Bacteria.
DR   HOGENOM; CLU_069513_0_0_6; -.
DR   OMA; YRLSTPQ; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR003653; Peptidase_C48_C.
DR   Pfam; PF02902; Peptidase_C48; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease.
FT   CHAIN           1..407
FT                   /note="Protease ElaD"
FT                   /id="PRO_0000323569"
FT   ACT_SITE        231
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        317
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   407 AA;  46648 MW;  B8BCD1F6328A654C CRC64;
     MMVTVVSNYC QLSQTQLSQT FAEKFTVTEE LLQSLKKTAL SGDEESIELL HNIALGYDEF
     GKKAEDILYH IVRNPTNDTL SIIKLIKNAC LKLYNLAHTA TKHPLKSHDS DNLLFKKLFS
     PSKLMAIIGE DIPLISEKQS LSKVLLNDKN NELSDGTNFW DKNRQLTTDE IACYLKKIAA
     NAKNTQVNYP TDFYLPNSNS TYLEVALNDN IKSDPSWPKE VQLFPINTGG HWILVSLQKI
     VNEKNNTQQI KCIIFNSLRA LGHEKENSLK RIINSFNSFN CDPTRETPNN KNITDHLTEP
     EIIFLHADLQ QYLSQSCGAF VCMAAQEVIE QMESNSDSAP YTLLKNYADR FKKYSAEEQY
     EIDFQHRLEN RNCYLDKYGD ANINHYYRNL EIKNSHPKNR ASSKRVS