ELAV3_HUMAN
ID ELAV3_HUMAN Reviewed; 367 AA.
AC Q14576; Q16135; Q96CL8; Q96QS9;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 3.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=ELAV-like protein 3;
DE AltName: Full=Hu-antigen C;
DE Short=HuC;
DE AltName: Full=Paraneoplastic cerebellar degeneration-associated antigen;
DE AltName: Full=Paraneoplastic limbic encephalitis antigen 21;
GN Name=ELAVL3; Synonyms=HUC, PLE21;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RA Manley T., Furneaux H.M.;
RL Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Zhang B., Yuan J.G., Qiang B.Q.;
RT "Isolation of HuC cDNA.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Brain;
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-367 (ISOFORM 2).
RC TISSUE=Hippocampus;
RX PubMed=7511893; DOI=10.1006/bbrc.1994.1358;
RA Sakai K., Gofuku M., Kitagawa Y., Ogasawara T., Hirose G., Yamazaki M.,
RA Koh C.S., Yanagisawa N., Steinman L.;
RT "A hippocampal protein associated with paraneoplastic neurologic syndrome
RT and small cell lung carcinoma.";
RL Biochem. Biophys. Res. Commun. 199:1200-1208(1994).
RN [6]
RP FUNCTION.
RX PubMed=10710437; DOI=10.1093/nar/28.7.e20;
RA King P.H.;
RT "RNA-binding analyses of HuC and HuD with the VEGF and c-myc 3'-
RT untranslated regions using a novel ELISA-based assay.";
RL Nucleic Acids Res. 28:E20-E20(2000).
CC -!- FUNCTION: RNA-binding protein that binds to AU-rich element (ARE)
CC sequences of target mRNAs, including VEGF mRNA (PubMed:10710437). May
CC also bind poly-A tracts via RRM 3 (By similarity). May be involved in
CC neuronal differentiation and maintenance (By similarity). Plays a role
CC in the stabilization of GAP43 mRNA and in spatial learning (By
CC similarity). {ECO:0000250|UniProtKB:Q60900,
CC ECO:0000269|PubMed:10710437}.
CC -!- SUBUNIT: Interacts with MAP1B light chain LC1.
CC {ECO:0000250|UniProtKB:Q60900}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q14576-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q14576-2; Sequence=VSP_005789;
CC -!- TISSUE SPECIFICITY: Brain specific.
CC -!- DOMAIN: RRM 1 and RRM 2 bind cooperatively to AU-rich sequences in
CC target mRNAs. RRM 3 binds to poly-A mRNA sequences (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRM elav family. {ECO:0000305}.
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DR EMBL; L26405; AAA58677.1; -; mRNA.
DR EMBL; AY036909; AAK67714.1; -; mRNA.
DR EMBL; AC008481; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC014144; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; D26158; BAA21838.1; -; mRNA.
DR CCDS; CCDS32912.1; -. [Q14576-1]
DR CCDS; CCDS45978.1; -. [Q14576-2]
DR RefSeq; NP_001411.2; NM_001420.3. [Q14576-1]
DR RefSeq; NP_115657.2; NM_032281.2. [Q14576-2]
DR AlphaFoldDB; Q14576; -.
DR SMR; Q14576; -.
DR BioGRID; 108310; 16.
DR IntAct; Q14576; 8.
DR STRING; 9606.ENSP00000352162; -.
DR BindingDB; Q14576; -.
DR ChEMBL; CHEMBL4105924; -.
DR iPTMnet; Q14576; -.
DR PhosphoSitePlus; Q14576; -.
DR BioMuta; ELAVL3; -.
DR DMDM; 21264436; -.
DR EPD; Q14576; -.
DR jPOST; Q14576; -.
DR MassIVE; Q14576; -.
DR MaxQB; Q14576; -.
DR PaxDb; Q14576; -.
DR PeptideAtlas; Q14576; -.
DR PRIDE; Q14576; -.
DR ProteomicsDB; 60054; -. [Q14576-1]
DR ProteomicsDB; 60055; -. [Q14576-2]
DR Antibodypedia; 25887; 177 antibodies from 27 providers.
DR DNASU; 1995; -.
DR Ensembl; ENST00000359227.8; ENSP00000352162.1; ENSG00000196361.10. [Q14576-1]
DR Ensembl; ENST00000438662.6; ENSP00000390878.1; ENSG00000196361.10. [Q14576-2]
DR GeneID; 1995; -.
DR KEGG; hsa:1995; -.
DR MANE-Select; ENST00000359227.8; ENSP00000352162.1; NM_001420.4; NP_001411.2.
DR UCSC; uc002mrx.2; human. [Q14576-1]
DR CTD; 1995; -.
DR DisGeNET; 1995; -.
DR GeneCards; ELAVL3; -.
DR HGNC; HGNC:3314; ELAVL3.
DR HPA; ENSG00000196361; Tissue enriched (brain).
DR MIM; 603458; gene.
DR neXtProt; NX_Q14576; -.
DR OpenTargets; ENSG00000196361; -.
DR PharmGKB; PA27742; -.
DR VEuPathDB; HostDB:ENSG00000196361; -.
DR eggNOG; KOG0145; Eukaryota.
DR GeneTree; ENSGT00940000160389; -.
DR HOGENOM; CLU_026186_2_2_1; -.
DR InParanoid; Q14576; -.
DR OMA; AGCTEPI; -.
DR OrthoDB; 614259at2759; -.
DR PhylomeDB; Q14576; -.
DR TreeFam; TF313377; -.
DR PathwayCommons; Q14576; -.
DR SignaLink; Q14576; -.
DR SIGNOR; Q14576; -.
DR BioGRID-ORCS; 1995; 10 hits in 1063 CRISPR screens.
DR ChiTaRS; ELAVL3; human.
DR GeneWiki; ELAVL3; -.
DR GenomeRNAi; 1995; -.
DR Pharos; Q14576; Tchem.
DR PRO; PR:Q14576; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q14576; protein.
DR Bgee; ENSG00000196361; Expressed in inferior vagus X ganglion and 187 other tissues.
DR ExpressionAtlas; Q14576; baseline and differential.
DR Genevisible; Q14576; HS.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:InterPro.
DR GO; GO:0035925; F:mRNA 3'-UTR AU-rich region binding; IDA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR CDD; cd12650; RRM1_Hu; 1.
DR CDD; cd12655; RRM3_HuC; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR006548; ELAD_HU_SF.
DR InterPro; IPR034775; ELAV/Hu_RRM1.
DR InterPro; IPR034915; HuC_RRM3.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR Pfam; PF00076; RRM_1; 3.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 3.
DR SMART; SM00361; RRM_1; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01661; ELAV_HUD_SF; 1.
DR PROSITE; PS50102; RRM; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation; Neurogenesis;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..367
FT /note="ELAV-like protein 3"
FT /id="PRO_0000081581"
FT DOMAIN 39..117
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 125..205
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 284..362
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT VAR_SEQ 251..257
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:7511893, ECO:0000303|Ref.1,
FT ECO:0000303|Ref.2"
FT /id="VSP_005789"
FT CONFLICT 20..25
FT /note="PALPNG -> RPAQR (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 78..79
FT /note="QS -> RD (in Ref. 1; AAA58677 and 2; AAK67714)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="S -> P (in Ref. 1; AAA58677 and 2; AAK67714)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="A -> R (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="G -> A (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 282
FT /note="Missing (in Ref. 1; AAA58677 and 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 295..297
FT /note="ADE -> PDQ (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 330
FT /note="V -> M (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
FT CONFLICT 351
FT /note="G -> A (in Ref. 1; AAA58677)"
FT /evidence="ECO:0000305"
FT CONFLICT 352
FT /note="E -> Q (in Ref. 4; BAA21838)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 39547 MW; 03FB3BD4D112B326 CRC64;
MVTQILGAME SQVGGGPAGP ALPNGPLLGT NGATDDSKTN LIVNYLPQNM TQDEFKSLFG
SIGDIESCKL VRDKITGQSL GYGFVNYSDP NDADKAINTL NGLKLQTKTI KVSYARPSSA
SIRDANLYVS GLPKTMSQKE MEQLFSQYGR IITSRILVDQ VTGVSRGVGF IRFDKRIEAE
EAIKGLNGQK PLGAAEPITV KFANNPSQKT GQALLTHLYQ SSARRYAGPL HHQTQRFRLD
NLLNMAYGVK SPLSLIARFS PIAIDGMSGL AGVGLSGGAA GAGWCIFVYN LSPEADESVL
WQLFGPFGAV TNVKVIRDFT TNKCKGFGFV TMTNYDEAAM AIASLNGYRL GERVLQVSFK
TSKQHKA
