ELBA2_DROME
ID ELBA2_DROME Reviewed; 381 AA.
AC Q9VQD6;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Early boundary activity protein 2;
GN Name=Elba2; ORFNames=CG9883;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, DOMAIN BEN, AND DNA-BINDING.
RX PubMed=23240086; DOI=10.7554/elife.00171;
RA Aoki T., Sarkeshik A., Yates J., Schedl P.;
RT "Elba, a novel developmentally regulated chromatin boundary factor is a
RT hetero-tripartite DNA binding complex.";
RL Elife 1:E00171-E00171(2012).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DNA-BINDING, AND
RP DOMAIN BEN.
RX PubMed=25561495; DOI=10.1101/gad.252122.114;
RA Dai Q., Ren A., Westholm J.O., Duan H., Patel D.J., Lai E.C.;
RT "Common and distinct DNA-binding and regulatory activities of the BEN-solo
RT transcription factor family.";
RL Genes Dev. 29:48-62(2015).
CC -!- FUNCTION: The heterotrimeric Elba complex is required for chromatin
CC domain boundary function during early embryogenesis. It binds to a 8-bp
CC sequence 5'-CCAATAAG-3' in the Fab-7 insulator or boundary element in
CC the bithorax complex and contributes to its insulator or boundary
CC activity (PubMed:23240086). Elba2 can act as a transcriptional
CC repressor and binds the palindromic sequence 5'-CCAATTGG-3' to mediate
CC transcriptional repression (PubMed:25561495).
CC {ECO:0000269|PubMed:23240086, ECO:0000269|PubMed:25561495}.
CC -!- SUBUNIT: The heterotrimeric Elba complex consists of Bsg25A/Elba1,
CC Elba2 and Elba3. {ECO:0000269|PubMed:23240086}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23240086,
CC ECO:0000269|PubMed:25561495}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout much embryogenesis.
CC Expression peaks at the blastoderm stage (2-4 hours) and at later
CC embryonic stages expression corresponds mostly to the procephalic
CC ectoderm primordium. {ECO:0000269|PubMed:23240086,
CC ECO:0000269|PubMed:25561495}.
CC -!- DOMAIN: The BEN domain mediates DNA-binding.
CC {ECO:0000269|PubMed:23240086, ECO:0000269|PubMed:25561495}.
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DR EMBL; AE003582; AAF51239.1; -; Genomic_DNA.
DR EMBL; AY069412; AAL39557.1; -; mRNA.
DR RefSeq; NP_608691.1; NM_134847.3.
DR AlphaFoldDB; Q9VQD6; -.
DR SMR; Q9VQD6; -.
DR IntAct; Q9VQD6; 23.
DR STRING; 7227.FBpp0077377; -.
DR PaxDb; Q9VQD6; -.
DR DNASU; 33442; -.
DR EnsemblMetazoa; FBtr0077693; FBpp0077377; FBgn0031435.
DR GeneID; 33442; -.
DR KEGG; dme:Dmel_CG9883; -.
DR UCSC; CG9883-RA; d. melanogaster.
DR CTD; 33442; -.
DR FlyBase; FBgn0031435; Elba2.
DR VEuPathDB; VectorBase:FBgn0031435; -.
DR eggNOG; ENOG502T9C9; Eukaryota.
DR HOGENOM; CLU_061882_0_0_1; -.
DR InParanoid; Q9VQD6; -.
DR OMA; QYGEVFW; -.
DR OrthoDB; 983061at2759; -.
DR PhylomeDB; Q9VQD6; -.
DR SignaLink; Q9VQD6; -.
DR BioGRID-ORCS; 33442; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33442; -.
DR PRO; PR:Q9VQD6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031435; Expressed in adult abdomen and 35 other tissues.
DR Genevisible; Q9VQD6; DM.
DR GO; GO:0005677; C:chromatin silencing complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005704; C:polytene chromosome band; IDA:FlyBase.
DR GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; IMP:FlyBase.
DR GO; GO:0033696; P:heterochromatin boundary formation; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR InterPro; IPR018379; BEN_domain.
DR Pfam; PF10523; BEN; 1.
DR SMART; SM01025; BEN; 1.
DR PROSITE; PS51457; BEN; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..381
FT /note="Early boundary activity protein 2"
FT /id="PRO_0000434593"
FT DOMAIN 268..365
FT /note="BEN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
FT REGION 210..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 381 AA; 43310 MW; 3CB0225274A0CE62 CRC64;
MAGGFRPYDQ DSCPNLTNSE APEFSMSVVC KTEPHYFHPQ TAILPSVQYS HPYHHYLSQF
APNFVPYYYR LLSRPIMKQE EMDIENYINY EVAAQQTMMR QRTLKPLGQL QIQMPPPIIV
NQPVKPVPVK AVPVRRSSPP KRRVINAQLV AVATASGGIK NIEPRVEPLP RLEESFKQQV
AKIQKSQHHY EQLFGRLTSM LKTLNQRYDN DAEDVPAPPS KRPRHMSTSS SESHIPDTAS
EKDEKDTLVQ YPHRVQKEDG SAVYVLGPNG TQITAHQYGE VFWTNAPVAT RCLLCVVFSS
DELATHTLTG KPSPAFYGRE RPPKLQLDQR KVDDIVVCVR NRTGGKERVI RATITTKCAD
TAKKYKRRAK KAQKVAIKEE Y
