ELBA3_DROME
ID ELBA3_DROME Reviewed; 379 AA.
AC Q9VR19;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Early boundary activity protein 3;
GN Name=Elba3; ORFNames=CG15634;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=23240086; DOI=10.7554/elife.00171;
RA Aoki T., Sarkeshik A., Yates J., Schedl P.;
RT "Elba, a novel developmentally regulated chromatin boundary factor is a
RT hetero-tripartite DNA binding complex.";
RL Elife 1:E00171-E00171(2012).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=25561495; DOI=10.1101/gad.252122.114;
RA Dai Q., Ren A., Westholm J.O., Duan H., Patel D.J., Lai E.C.;
RT "Common and distinct DNA-binding and regulatory activities of the BEN-solo
RT transcription factor family.";
RL Genes Dev. 29:48-62(2015).
CC -!- FUNCTION: The heterotrimeric Elba complex is required for chromatin
CC domain boundary function during early embryogenesis. It binds to a 8-bp
CC sequence 5'-CCAATAAG-3' in the Fab-7 insulator or boundary element in
CC the bithorax complex and contributes to its insulator or boundary
CC activity. Elba3 lacks DNA-binding activity and plays the role of an
CC adapter protein, bringing Elba1 and 2 together, thereby establishing a
CC complex that recognizes the asymmetric sequence motif through the BEN
CC domains of Elba1 and 2 (PubMed:23240086).
CC {ECO:0000269|PubMed:23240086}.
CC -!- SUBUNIT: The heterotrimeric Elba complex consists of Bsg25A/Elba1,
CC Elba2 and Elba3. {ECO:0000269|PubMed:23240086}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23240086}.
CC -!- DEVELOPMENTAL STAGE: Expression is developmentally restricted, peaks at
CC the blastoderm stage (2-4 hours) and then disappears (at protein
CC level). {ECO:0000269|PubMed:23240086, ECO:0000269|PubMed:25561495}.
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DR EMBL; AE014134; AAF50989.1; -; Genomic_DNA.
DR EMBL; AY051954; AAK93378.1; -; mRNA.
DR RefSeq; NP_608850.1; NM_135006.3.
DR AlphaFoldDB; Q9VR19; -.
DR IntAct; Q9VR19; 8.
DR STRING; 7227.FBpp0077070; -.
DR PaxDb; Q9VR19; -.
DR PRIDE; Q9VR19; -.
DR DNASU; 33670; -.
DR EnsemblMetazoa; FBtr0077378; FBpp0077070; FBgn0031621.
DR GeneID; 33670; -.
DR KEGG; dme:Dmel_CG15634; -.
DR UCSC; CG15634-RA; d. melanogaster.
DR CTD; 33670; -.
DR FlyBase; FBgn0031621; Elba3.
DR VEuPathDB; VectorBase:FBgn0031621; -.
DR eggNOG; ENOG502T8EF; Eukaryota.
DR HOGENOM; CLU_730109_0_0_1; -.
DR InParanoid; Q9VR19; -.
DR OMA; RCFDHAF; -.
DR OrthoDB; 896102at2759; -.
DR PhylomeDB; Q9VR19; -.
DR BioGRID-ORCS; 33670; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33670; -.
DR PRO; PR:Q9VR19; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031621; Expressed in cleaving embryo and 4 other tissues.
DR Genevisible; Q9VR19; DM.
DR GO; GO:0005677; C:chromatin silencing complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IDA:FlyBase.
DR GO; GO:0033696; P:heterochromatin boundary formation; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..379
FT /note="Early boundary activity protein 3"
FT /id="PRO_0000434592"
SQ SEQUENCE 379 AA; 43008 MW; 9132C62BB2B7EDB1 CRC64;
MSNISNDSGL DDSANSGAVV SANGPLAATR LSWFLAEVDD GLMKDGKPNG RRRLCVLHSM
ELLESDVSDK YMTRFVEFRV NGMVLEAKLI LAADERRLVD AALLSMSKEE REDAGGQQLL
VQYTENEKAG ERLIQKLVSP NNVMLLSTKP ELKGNPLVRL APGCIAHILY AYESRDYMEK
ILLHLKARSF DLAFDDNLEA EPEAMNDDTW MLVQYSPEPE MVVYQVVQYR QTVWRKENLF
KDVIAYMQLP GSDIVLQAVV ISYGQDKEVQ DAKYEELQRF SFDIDFPLPE ELEKHPDHMT
STALFSRTSL YQKAEQRDTT GEHRELRKSL EQMSEKAQGE AQMIIDAFDM VDNINKNLQS
RLSGEVRSVV EVTSGDELH
