ID   ELDP1_PINMA             Reviewed;         348 AA.
AC   P86953;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   10-FEB-2021, entry version 19.
DE   RecName: Full=EGF-like domain-containing protein 1;
DE   Flags: Precursor; Fragment;
OS   Pinctada maxima (Silver-lipped pearl oyster) (White-lipped pearl oyster).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=104660;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|PubMed:19915030};
RX   PubMed=19915030; DOI=10.1093/molbev/msp278;
RA   Jackson D.J., McDougall C., Woodcroft B., Moase P., Rose R.A., Kube M.,
RA   Reinhardt R., Rokhsar D.S., Montagnani C., Joubert C., Piquemal D.,
RA   Degnan B.M.;
RT   "Parallel evolution of nacre building gene sets in molluscs.";
RL   Mol. Biol. Evol. 27:591-608(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 32-55; 146-157 AND 187-215, SUBCELLULAR LOCATION, AND
RP   TISSUE SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23213212; DOI=10.1073/pnas.1210552109;
RA   Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C.,
RA   Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.;
RT   "Different secretory repertoires control the biomineralization processes of
RT   prism and nacre deposition of the pearl oyster shell.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23213212}.
CC   -!- TISSUE SPECIFICITY: Prismatic layer of shell (at protein level).
CC       Expressed primarily in the mantle with highest level in the mantle edge
CC       and lower level in the mantle pallium. {ECO:0000269|PubMed:23213212}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=GT282449; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; GT278041; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT282449; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT284230; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; EZ420398; -; NOT_ANNOTATED_CDS; mRNA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR001507; ZP_dom.
DR   PROSITE; PS00022; EGF_1; 2.
DR   PROSITE; PS50026; EGF_3; 1.
DR   PROSITE; PS51034; ZP_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; EGF-like domain; Secreted;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..348
FT                   /note="EGF-like domain-containing protein 1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000413070"
FT   DOMAIN          60..92
FT                   /note="EGF-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DOMAIN          99..342
FT                   /note="ZP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00375"
FT   DISULFID        64..74
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        68..80
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        82..91
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   CONFLICT        159
FT                   /note="H -> R (in Ref. 1; GT278041)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        162
FT                   /note="I -> M (in Ref. 1; EZ420398/GT278041)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         348
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   348 AA;  38362 MW;  78A2C7C8035401AE CRC64;
     MFYLSTFMTI VISLSLVSCS YDCNNPGYSC KGTCHYYGPC ICNEKLMGYD CSVLKSRMST
     GSNCTVTCQN NGKCYDGSKC LCSSDYTGDL CEKQTTGARC TLDAVVFEAY RPIGFVGETY
     LSQSRSCKLL ETTSDVPGMI KFERKIFHGD TSMCGLKKHM DIPSAGDVTY EADIYSTFQY
     NSWGTRDFMD NVKCQYKPTR VGLSMDAPDS LFPIKMSARD GASSNVQATT QSAPISLLFS
     PQNIPDVKGA MVDYLEVYSI NSTSKEYKSV VAVKNGCAQK NEYNVAFSNL DELDPATSKW
     IGLVKMQAFI IFENEPILFN YRLRFCPDRC TTPTCAAPXV GQATSAAV