ELF3_ARATH
ID ELF3_ARATH Reviewed; 695 AA.
AC O82804; O04419; Q8L7A2;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Protein EARLY FLOWERING 3;
DE AltName: Full=Nematode-responsive protein;
GN Name=ELF3; Synonyms=PYK20; OrderedLocusNames=At2g25930;
GN ORFNames=F17H15.25, T19L18.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. C24; TISSUE=Shoot;
RX PubMed=10571046; DOI=10.1016/s0378-1119(99)00353-4;
RA Puzio P.S., Lausen J., Almeida-Engler J., Cai D., Gheysen G.,
RA Grundler F.M.W.;
RT "Isolation of a gene from Arabidopsis thaliana related to nematode feeding
RT structures.";
RL Gene 239:163-172(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1), AND MUTANT ELF3-7.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=11402160; DOI=10.2307/3871295;
RA Hicks K.A., Albertson T.M., Wagner D.R.;
RT "EARLY FLOWERING3 encodes a novel protein that regulates circadian clock
RT function and flowering in Arabidopsis.";
RL Plant Cell 13:1281-1292(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION.
RX PubMed=11402161; DOI=10.2307/3871296;
RA Liu X.L., Covington M.F., Fankhauser C., Chory J., Wagner D.R.;
RT "ELF3 encodes a circadian clock-regulated nuclear protein that functions in
RT an Arabidopsis PHYB signal transduction pathway.";
RL Plant Cell 13:1293-1304(2001).
RN [7]
RP FUNCTION, INTERACTION WITH ELF4, AND SUBCELLULAR LOCATION.
RX PubMed=22327739; DOI=10.1105/tpc.111.093807;
RA Herrero E., Kolmos E., Bujdoso N., Yuan Y., Wang M., Berns M.C.,
RA Uhlworm H., Coupland G., Saini R., Jaskolski M., Webb A., Goncalves J.,
RA Davis S.J.;
RT "EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains
RT the Arabidopsis circadian clock.";
RL Plant Cell 24:428-443(2012).
RN [8]
RP FUNCTION.
RX PubMed=22307044; DOI=10.4161/psb.18766;
RA Chow B.Y., Helfer A., Nusinow D.A., Kay S.A.;
RT "ELF3 recruitment to the PRR9 promoter requires other Evening Complex
RT members in the Arabidopsis circadian clock.";
RL Plant Signal. Behav. 7:170-173(2012).
CC -!- FUNCTION: May be a transcription factor part of a circadian clock input
CC pathway. Acts within a 'zeitnehmer' feedback loop and is involved in
CC its own circadian regulation. Has no role in regulating circadian clock
CC function in the dark. Part of a corepressor complex consisting of ELF4,
CC ELF3, and LUX involved in the transcriptional regulation of APRR9. The
CC activity of the protein may be decreased in long day conditions due to
CC its interaction with phytochrome B (phyB). Can regulate the initiation
CC of flowering independently of phyB. Also involved in responses to
CC nematode parasitism, like the formation of the nematode feeding
CC structure. {ECO:0000269|PubMed:11402161, ECO:0000269|PubMed:22307044,
CC ECO:0000269|PubMed:22327739}.
CC -!- SUBUNIT: Interacts specifically with both Pr and Pfr forms of
CC phytochrome B. Interacts with ELF4. May form a homodimer.
CC {ECO:0000269|PubMed:22327739}.
CC -!- INTERACTION:
CC O82804; O04211: ELF4; NbExp=4; IntAct=EBI-16415030, EBI-16415004;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22327739}. Note=Forms
CC nuclear bodies. {ECO:0000269|PubMed:22327739}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O82804-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O82804-2; Sequence=VSP_004042, VSP_004043;
CC -!- INDUCTION: Expressed with a circadian rhythm showing a peak 14 to 16
CC hours after sunrise regardless of day length. Induced in roots after
CC infection by nematodes. Up-regulated by auxin and cytokinin and down-
CC regulated by abscisic acid and temperature stress.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA72719.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Y11994; CAA72719.1; ALT_FRAME; mRNA.
DR EMBL; AC004747; AAC31242.1; -; Genomic_DNA.
DR EMBL; AC005395; AAM15042.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07774.1; -; Genomic_DNA.
DR EMBL; AY136385; AAM97051.1; -; mRNA.
DR EMBL; BT000185; AAN15504.1; -; mRNA.
DR PIR; T02630; T02630.
DR PIR; T52634; T52634.
DR RefSeq; NP_180164.1; NM_128153.3. [O82804-1]
DR AlphaFoldDB; O82804; -.
DR BioGRID; 2486; 41.
DR ComplexPortal; CPX-1291; Evening Complex.
DR IntAct; O82804; 16.
DR STRING; 3702.AT2G25930.1; -.
DR PaxDb; O82804; -.
DR PRIDE; O82804; -.
DR ProteomicsDB; 221941; -. [O82804-1]
DR EnsemblPlants; AT2G25930.1; AT2G25930.1; AT2G25930. [O82804-1]
DR GeneID; 817134; -.
DR Gramene; AT2G25930.1; AT2G25930.1; AT2G25930. [O82804-1]
DR KEGG; ath:AT2G25930; -.
DR Araport; AT2G25930; -.
DR TAIR; locus:2043520; AT2G25930.
DR eggNOG; ENOG502QSB6; Eukaryota.
DR HOGENOM; CLU_027835_1_0_1; -.
DR InParanoid; O82804; -.
DR OMA; MECNAEN; -.
DR OrthoDB; 245514at2759; -.
DR PhylomeDB; O82804; -.
DR PRO; PR:O82804; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82804; baseline and differential.
DR Genevisible; O82804; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005667; C:transcription regulator complex; IDA:ComplexPortal.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:TAIR.
DR GO; GO:0007623; P:circadian rhythm; IMP:TAIR.
DR GO; GO:0010031; P:circumnutation; IMP:TAIR.
DR GO; GO:0009649; P:entrainment of circadian clock; IMP:TAIR.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:TAIR.
DR GO; GO:0009585; P:red, far-red light phototransduction; IMP:TAIR.
DR GO; GO:0042752; P:regulation of circadian rhythm; IDA:ComplexPortal.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IEA:InterPro.
DR GO; GO:0010119; P:regulation of stomatal movement; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:TAIR.
DR InterPro; IPR039319; ELF3-like.
DR PANTHER; PTHR34281; PTHR34281; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Biological rhythms; Nucleus;
KW Phytochrome signaling pathway; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..695
FT /note="Protein EARLY FLOWERING 3"
FT /id="PRO_0000086953"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..484
FT /note="Interaction with ELF3"
FT REGION 541..653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..156
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..258
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..283
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..613
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 628..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 339
FT /note="N -> K (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_004042"
FT VAR_SEQ 340..695
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_004043"
FT VARIANT 550
FT /note="Q -> QQQQQQQQQQ (in strain: cv. Wassilewskija)"
FT MUTAGEN 66..73
FT /note="Missing: In elf3-7; causes early flowering and long
FT hypocotyl phenotypes."
FT CONFLICT 55
FT /note="M -> R (in Ref. 1; CAA72719)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="A -> R (in Ref. 1; CAA72719)"
FT /evidence="ECO:0000305"
FT CONFLICT 618
FT /note="R -> G (in Ref. 1; CAA72719)"
FT /evidence="ECO:0000305"
FT CONFLICT 670
FT /note="K -> KVVPHNAK (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 695 AA; 77206 MW; 607A0720ED381C08 CRC64;
MKRGKDEEKI LEPMFPRLHV NDADKGGPRA PPRNKMALYE QLSIPSQRFG DHGTMNSRSN
NTSTLVHPGP SSQPCGVERN LSVQHLDSSA ANQATEKFVS QMSFMENVRS SAQHDQRKMV
REEEDFAVPV YINSRRSQSH GRTKSGIEKE KHTPMVAPSS HHSIRFQEVN QTGSKQNVCL
ATCSKPEVRD QVKANARSGG FVISLDVSVT EEIDLEKSAS SHDRVNDYNA SLRQESRNRL
YRDGGKTRLK DTDNGAESHL ATENHSQEGH GSPEDIDNDR EYSKSRACAS LQQINEEASD
DVSDDSMVDS ISSIDVSPDD VVGILGQKRF WRARKAIANQ QRVFAVQLFE LHRLIKVQKL
IAASPDLLLD EISFLGKVSA KSYPVKKLLP SEFLVKPPLP HVVVKQRGDS EKTDQHKMES
SAENVVGRLS NQGHHQQSNY MPFANNPPAS PAPNGYCFPP QPPPSGNHQQ WLIPVMSPSE
GLIYKPHPGM AHTGHYGGYY GHYMPTPMVM PQYHPGMGFP PPGNGYFPPY GMMPTIMNPY
CSSQQQQQQQ PNEQMNQFGH PGNLQNTQQQ QQRSDNEPAP QQQQQPTKSY PRARKSRQGS
TGSSPSGPQG ISGSKSFRPF AAVDEDSNIN NAPEQTMTTT TTTTRTTVTQ TTRDGGGVTR
VIKVVPHNAK LASENAARIF QSIQEERKRY DSSKP
