ID   ELFA_ECO57              Reviewed;         182 AA.
AC   Q8X582; Q7AG44;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Laminin-binding fimbrial subunit ElfA;
DE   AltName: Full=ELF;
DE   Flags: Precursor;
GN   Name=elfA; Synonyms=ycbQ; OrderedLocusNames=ECs1021, Z1286;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   FUNCTION, LAMININ-BINDING, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION
RP   PHENOTYPE, AND GENE NAME.
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=19508558; DOI=10.1111/j.1462-2920.2009.01906.x;
RA   Samadder P., Xicohtencatl-Cortes J., Saldana Z., Jordan D., Tarr P.I.,
RA   Kaper J.B., Giron J.A.;
RT   "The Escherichia coli ycbQRST operon encodes fimbriae with laminin-binding
RT   and epithelial cell adherence properties in Shiga-toxigenic E.coli
RT   O157:H7.";
RL   Environ. Microbiol. 11:1815-1826(2009).
CC   -!- FUNCTION: Part of the elfADCG fimbrial operon, which could be required
CC       for adherence to host epithelial cells. ElfA is an accessory
CC       colonization factor that contributes to adherence of bacteria to human
CC       intestinal epithelial cells and to animal intestinal tissue in vitro.
CC       Binds specifically to laminin, but not to fibronectin or collagen type
CC       IV. {ECO:0000269|PubMed:19508558}.
CC   -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000269|PubMed:19508558}.
CC   -!- INDUCTION: Induced in the presence of epithelial cells.
CC       {ECO:0000269|PubMed:19508558}.
CC   -!- DISRUPTION PHENOTYPE: Mutants show significant reduction in adherence
CC       to several cultured epithelial cells (HEp-2, HT-29 and MDBK).
CC       {ECO:0000269|PubMed:19508558}.
CC   -!- SIMILARITY: Belongs to the fimbrial protein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005174; AAG55423.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34444.1; -; Genomic_DNA.
DR   PIR; C85620; C85620.
DR   PIR; E90756; E90756.
DR   RefSeq; NP_309048.2; NC_002695.1.
DR   AlphaFoldDB; Q8X582; -.
DR   SMR; Q8X582; -.
DR   STRING; 155864.EDL933_1202; -.
DR   EnsemblBacteria; AAG55423; AAG55423; Z1286.
DR   EnsemblBacteria; BAB34444; BAB34444; ECs_1021.
DR   GeneID; 917768; -.
DR   KEGG; ece:Z1286; -.
DR   KEGG; ecs:ECs_1021; -.
DR   PATRIC; fig|386585.9.peg.1143; -.
DR   eggNOG; COG3539; Bacteria.
DR   HOGENOM; CLU_088965_0_0_6; -.
DR   OMA; APACTIA; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   Gene3D; 2.60.40.1090; -; 1.
DR   InterPro; IPR000259; Adhesion_dom_fimbrial.
DR   InterPro; IPR036937; Adhesion_dom_fimbrial_sf.
DR   InterPro; IPR008966; Adhesion_dom_sf.
DR   Pfam; PF00419; Fimbrial; 1.
DR   SUPFAM; SSF49401; SSF49401; 1.
PE   1: Evidence at protein level;
KW   Fimbrium; Reference proteome; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..182
FT                   /note="Laminin-binding fimbrial subunit ElfA"
FT                   /id="PRO_0000413217"
SQ   SEQUENCE   182 AA;  19040 MW;  73C5574103D53F97 CRC64;
     MITMKKSVLT AFITVVCATS SVMAADDNAI TDGSVTFNGK VIAPACTLVA ATKDSVVTLP
     DVSATKLQTN GQVSGVQTDV PIELKDCDTT VTKNATFTFN GTADTTQITA FANQASSDAA
     TNVALQMYMN DGTTAIKPDT ETGNILLQDG DQTLTFKVDY IATGKATSGN VNAVTNFHIN
     YY